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Molecular Biology Reports

, Volume 36, Issue 2, pp 281–287 | Cite as

Cloning and functional identification of two members of the ZIP (Zrt, Irt-like protein) gene family in rice (Oryza sativa L.)

  • Xia Yang
  • Ji Huang
  • Yan Jiang
  • Hong-Sheng ZhangEmail author
Article

Abstract

Two ZIP (Zrt, Irt-like Protein) cDNAs were isolated from rice (Oryza sativa L.) by RT-PCR approach, and named as OsZIP7a and OsZIP8 respectively. The predicted proteins of OsZIP7a and OsZIP8 consist of 384 and 390 amino acid residues respectively, and display high similarity to other plant ZIP proteins. Each protein contains eight transmembrane (TM) domains and a highly conserved ZIP signature motif, with a histidine-rich region in the variable region between TM domains III and IV. By semi-quantitative RT-PCR approach, it was found that the expression of OsZIP7a was significantly induced in rice roots by iron-deficiency, while that of OsZIP8 induced in both rice roots and shoots by zinc-deficiency. When expressed in yeast cells, OsZIP7a and OsZIP8 could complement an iron-uptake-deficient yeast mutant and a zinc-uptake-deficient yeast mutant respectively. It suggested that the OsZIP7a and OsZIP8 might encode an iron and a zinc transporter protein in rice respectively.

Keywords

Rice Iron/zinc Transporter ZIP (Zrt, Irt-like protein) Gene cloning Yeast complementation 

Abbreviations

BPDS

Bathophenanthroline disulphonic acid disodium salt

EDTA

Ethylenediaminetetraacetic acid

Irt

Iron regulated transporter

ORF

Opening reading frame

RT

Reverse transcripts

TM

Transmembrane

Zrt

Zinc regulated transporter

Notes

Acknowledgements

We were indebted to David Eide (Nutritional Science Program, University of Missouri, Columbia, USA) for providing the yeast strains DY1455, DEY1453, ZHY3 and yeast expression vector pFL61. This work was supported by Natural Science Foundation of Jiangsu Province (Grant No.BK2005090) and Jiangsu Provincial Three-Item of Agricultural Engineering (Grant No.SX(2006)123).

References

  1. 1.
    Marschner H (1985) Mineral nutrition of higher plants. Academic Press, San Diego, CAGoogle Scholar
  2. 2.
    Salgueiro MJ, Zubillaga M, Lysionek AE, Caro RA, Weill R, Boccio JR (2002) The role of zinc in the growth and development of children. Nutrition 18:510–519Google Scholar
  3. 3.
    Tapiero H, Gate L, Tew KD (2001) Iron: deficiencies and requirements. Biomed Pharmacother 55:324–332PubMedCrossRefGoogle Scholar
  4. 4.
    Ruel MT, Rivera JA, Santizo MC, Lonnerdal B, Brown KH (1997) Vitamin B-12 deficiency is very prevalent in lactating guatemalan women and their infants at three months postpartum. J Nutr 127:1966–1972PubMedGoogle Scholar
  5. 5.
    Maser P, Thomine S, Schroeder JI, Ward JM, Hirschi K, Sze H, Talke IN, Amtmann A, Maathuis FJM, Sanders D, Harper JF, Tchieu J, Gribskov M, Persans MW, Salt DE, Kim SA, Guerinot ML (2001) Phylogenetic relationships within cation transporter families of Arabidopis. Plant Physiol 126:1646–1667PubMedCrossRefGoogle Scholar
  6. 6.
    Guerinot ML (2000) The ZIP family of metal transporters. Biochim Biophys Acta 1465:190–198PubMedCrossRefGoogle Scholar
  7. 7.
    Grotz N, Fox T, Connolly E, Park W, Guerinot ML, Eide D (1998) Identification of a family of zinc transporter genes from Arabidopsis that respond to zinc deficiency. Proc Natl Acad Sci USA 95:7220–7224PubMedCrossRefGoogle Scholar
  8. 8.
    Vert G, Grotz N, Dedaldechamp F, Gaymard F, Guerinot ML, Briat JF, Curie C (2002) IRT1, an Arabidopsis transporter essential for iron uptake from the soil and for plant growth. Plant Cell 14:1223–1233PubMedCrossRefGoogle Scholar
  9. 9.
    Vert G, Briat JF, Curie C (2001) Arabidopsis IRT2 gene encodes a root-periphery iron transporter. Plant J 26:181–189PubMedCrossRefGoogle Scholar
  10. 10.
    Cohen CK, Fox TC, Garvin DF, Kochian LV (1998) The role of iron-deficiency stress responses in stimulating heavy-metal transport in plants. Plant Physiol 116(3):1063–1072PubMedCrossRefGoogle Scholar
  11. 11.
    Eckhardt U, Mas Marques A, Buckhout TJ (2001) Two iron-regulated cation transporters from tomato complement metal uptake-deficient yeast mutants. Plant Mol Biol 45:437–448PubMedCrossRefGoogle Scholar
  12. 12.
    Moreau S, Thomson RM, Kaiser BN, Trevaskis B, Guerinot ML, Udvardi MK, Puppo A, Day DA (2002) GmZIP1 encodes a symbiosis-specific zinc transporter in soybean. J Biol Chem 277:4738–4746PubMedCrossRefGoogle Scholar
  13. 13.
    Bughio N, Yamaguchi H, Nishizawa NK, Nakanishi H, Mori S (2002) Cloning an iron-regulated metal transporter from rice. J Exp Bot 53:1677–1682PubMedCrossRefGoogle Scholar
  14. 14.
    Ramesh SA, Shin R, Eide DJ, Schachtman P (2003) Differential metal selectivity and gene expression of two zinc transporters from rice. Plant Physiol 133:126–134PubMedCrossRefGoogle Scholar
  15. 15.
    Ishimaru Y, Suzuki M, Kobayashi T, Takahashi M, Nakanishi H, Mori S, Nishizawa NK (2005) OsZIP4, a novel zinc-regulated zinc transporter in rice. J Exp Bot 56:3207–3214PubMedCrossRefGoogle Scholar
  16. 16.
    Ishimaru Y, Suzuki M, Tsukamoto T, Suzuki K, Nakazono M, Kobayashi T, Wada Y, Watanabe S, Matsuhashi S, Takahashi M, Nakanishi H, Mori S, Nishizawa NK (2006) Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+. Plant J 45:335–346PubMedCrossRefGoogle Scholar
  17. 17.
    Burleigh SH, Kristensen BK, Bechmann IE (2003) A plasma membrane zinc transporter from Medicago truncatula is up-regulated in roots by Zn fertilization, yet down-regulated by arbuscular mycorrhizal colonization. Plant Mol Biol 52:1077–1088PubMedCrossRefGoogle Scholar
  18. 18.
    Gaither LA, Eide DJ (2001) Eukaryotic zinc transporters and their regulation. Biometals 14:251–270PubMedCrossRefGoogle Scholar
  19. 19.
    Reece KS, Mcelroy D, Wu R (1990) Genomic nucleotide sequence of four rice (Oryza sativa) actin genes. Plant Mol Biol 14:621–624PubMedCrossRefGoogle Scholar
  20. 20.
    Huang J, Wang JF, Zhang HS (2005) Rice ZFP15 gene encoding for a novel C2H2-type zinc finger protein lacking DLN box, is regulated by spike development but not by abiotic stresses. Mol Biol Rep 32:177–183PubMedCrossRefGoogle Scholar
  21. 21.
    Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882CrossRefGoogle Scholar
  22. 22.
    Nicholas KB, Nicholas HB, Deerfield DW (1997) GeneDoc: analysis and visualization of genetic variation. EMBNEW. News 4:14Google Scholar
  23. 23.
    Krogh A, Larsson B, von Heijne G, Sonnhammer EL (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 305:567–580PubMedCrossRefGoogle Scholar
  24. 24.
    Kumar S, Tamura K, Jacobsen I, Nei M (2000) MEGA2, Molecular evolutionary genetics analysis, Version 2.0. Pennsylvania State University, University Park, PA, and Arizona State Universities, Tempe, AZGoogle Scholar
  25. 25.
    Minet M, Dufour ME, Lacroute F (1992) Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs. Plant J 2:417–422PubMedGoogle Scholar
  26. 26.
    Zhao H, Eide D (1996) The yeast ZRT1 gene encodes the zinc transporter protein of a high-affinity uptake system induced by zinc limitation. Proc Natl Acad Sci USA 93:2454–2458PubMedCrossRefGoogle Scholar
  27. 27.
    Zhao H, Eide D (1996) The ZRT2 gene encodes the low affinity zinc transporter in Saccharomyces cerevisiae. J Biol Chem 271:23203–23210PubMedCrossRefGoogle Scholar
  28. 28.
    Gietz RD, Schiestl RH (1991) Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acids as carrier. Yeast 7:253–263PubMedCrossRefGoogle Scholar
  29. 29.
    Sherman F (1991) Getting started with yeast. Meth Enzymol 194:3–20PubMedCrossRefGoogle Scholar
  30. 30.
    Eide DJ (1998) The molecular biology of metal ion transport in Saccaromyces cerevisiae. Ann Rev Nutr 18:441–469CrossRefGoogle Scholar
  31. 31.
    van der Zaal BJ, Neuteboom LW, Pinas JE, Chardonnens AN, Schat H, Verkleij JA, Hooykaas PJ (1999) Overexpression of a novel Arabidopsis gene related to putative zinc-transporter genes from animals can lead to enhanced zinc resistance and accumulation. Plant Physiol 119:1047–1055PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Xia Yang
    • 1
  • Ji Huang
    • 1
  • Yan Jiang
    • 1
  • Hong-Sheng Zhang
    • 1
    Email author
  1. 1.State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingChina

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