Overproduction of mouse estrogen receptor alpha-ligand binding domain decreases bacterial growth
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Escherichia coli (E. coli) is the most widely used prokaryotic host system for the synthesis of recombinant proteins. The overproduction of recombinant proteins is sometimes lethal to the host cells. In the present study, we expressed the ligand binding domain (LBD) of mouse estrogen receptor alpha (mouse ERα) using an expression vector (pIVEX) in E. coli BL21(DE3) and examined the effect of production of this protein on bacterial growth. The expressed protein was immunologically detected as a 30 kD histidine-tagged protein in the soluble part of the bacterial lysate. The overproduction of mouse ERα-LBD, as reflected by total protein content and expression pattern, resulted in the decrease of bacterial growth.
KeywordsEstrogen receptor alpha Ligand binding domain Protein Overproduction Bacterial growth Host cell
The authors would like to thank Dr Vincent Giguere for providing the plasmid pCMX-mouse ERα cDNA and Dr. W. Nickle for kind gift of expression vector pIVEX. Swati Ghosh is a recipient of Senior Research Fellowship from the University Grants Commission, India. This work was supported by grants from the Department of Biotechnology (BT/PR3593/Med/14/468/2003), Government of India to M.K.T.
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