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Molecular Biology Reports

, Volume 35, Issue 1, pp 51–57 | Cite as

Probing RNA–antibiotic interactions: a FTIR study

  • J. Prathiba
  • R. Malathi
Original Paper

Abstract

The crystal structure determination of antibiotic binding sites on the 30S ribosomal subunit and the increasing demand for developing RNA-based drugs has prompted us to study the direct binding of spectinomycin, vancomycin and bleomycin with yeast total RNA using Fourier transform infrared (FTIR) spectroscopy. We report that the OH of spectinomycin and the peptide group of vancomycin can bind to the bases of RNA, which might depend on Mg2+ concentration. Bleomycin on the other hand does not show such a drastic effect on yeast total RNA. This study might help in developing innovative strategies utilizing RNA molecules to perform a variety of essential biological functions.

Keywords

FTIR Spectinomycin Vancomycin Bleomycin RNA–antibiotic interaction 

Notes

Acknowledgement

We thank Prof. R. Raghunathan, Head, Department of Organic Chemistry for his help in the FTIR studies.

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  1. 1.Department of Genetics, Dr ALM Post Graduate Institute of Basic Medical SciencesUniversity of Madras, TaramaniChennaiIndia

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