Molecular Biology Reports

, Volume 32, Issue 4, pp 247–255 | Cite as

Identification of a New Transcript Specifically Expressed in Mouse Spermatocytes: mmrp2

  • Shanye Gu
  • Jiarui Hu
  • Ping Song
  • Wuming Gong
  • Ming Guo
Article

Abstract

In an effort to examine the molecular basis of gametogenesis, we screened Riken cDNA datebase and the clone 4930481F22 that is expressed preponderantly in mouse testis was identified. In the course of the research, a new isoform of 4930481F22 clone was found, isolated from mouse testis and sequenced. It only lacks the 7th exon of 4930481F22 transcript. The new isoform only has 837 bp and encodes a putative 28.4 kDa protein. We investigated the expression pattern at the mRNA level by RT-PCR and in situ hybridization in testis. The new isoform was only expressed in the gonad, where it began to be detected at day 8 after birth. In situ hybridization proved that the new isoform mostly expressed in spermatocytes. The structure of the predicted protein and the expression pattern of the mRNA suggest that the new isoform could have an important role in meiosis. We temporarily named it mmrp 2 (Mouse Meiosis Related Protein 2).

Keywords

gonad-specific in situ hybridization RT-PCR sequence analysis spermatocyte 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Pellegrini, M, Grimaldi, P, Rossi, P, Geremia, R, Dolci, S 2003J. Cell Sci.11633633372PubMedGoogle Scholar
  2. 2.
    Chen, L, Sato, M, Inoko, H, Kimura, M 1997Biochem. Biophys. Res. Commun.240261268CrossRefPubMedGoogle Scholar
  3. 3.
    Clancy, MJ 1998Curr. Biol.8R461R463CrossRefPubMedGoogle Scholar
  4. 4.
    Dix, DJ, Allen, JW, Collins, BW, Poorman-Allen, P, Mori, C, Blizard, DR, Brown, PR, Goulding, EH, Strong, BD, Eddy, EM 1997Development12445954603PubMedGoogle Scholar
  5. 5.
    Sage, J, Martin, L, Meuwissen, R, Heyting, C, Cuzin, F, Rassoulzadegan, M 1999Development802939Google Scholar
  6. 6.
    Steiner, R, Ever, L, Don, J 1999Dev. Biol.216635645CrossRefPubMedGoogle Scholar
  7. 7.
    Rossi, P, Dolci, S, Sette, C, Capolunghi, F, Pellegrini, M, Loiarro, M, Di Agostino, S, Paronetto, MP, Grimaldi, P, Merico, D, Martegani, E, Geremia, R 2004Gene Exp. Patterns4267281Google Scholar
  8. 8.
    Frohman, MA, Dush, MK, Martin, GR 1988Proc. Natl. Acad. Sci.8589989002PubMedGoogle Scholar
  9. 9.
    Dijkman, HBPM, Mentzel, S., Jong, AS, Assmann, KJM 1995Biochemica Features22327No.Google Scholar
  10. 10.
    Guo, J, Zhu, P, Wu, C, Yu, L, Zhao, S, Gu, X 2003Cytogenet. Genome Res.1035862PubMedGoogle Scholar
  11. 11.
    Wolf, E, Kim, PS, Berger, B 1997Protein Sci.611791189PubMedGoogle Scholar
  12. 12.
    Mason, JM, Arndt, KM 2004Chembiochemistry5170176Google Scholar
  13. 13.
    Burkhard, P, Stetefeld, J, Strelkov, SV 2001Trends Cell Biol.118288CrossRefPubMedGoogle Scholar
  14. 14.
    Harvey SH, Krien MJ & O’Connell MJ (2002) Genome Biol. 3(2): reviews 3003.1–3003.5Google Scholar
  15. 15.
    Cobbe, N, Heck, MM 2000J. Struct. Biol.129123143CrossRefPubMedGoogle Scholar
  16. 16.
    Taylor, EM, Moghraby, JS, Lees, JH, Smit, B, Moens, PB, Lehmann, AR 2001Mol. Biol. Cell1215831594PubMedGoogle Scholar
  17. 17.
    Losada, A, Hirano, T 2001Bioessays23924935CrossRefPubMedGoogle Scholar
  18. 18.
    Zeng, L, Chen, Y, Lee, WH 1999Mol. Cell. Biol.1954175428Google Scholar
  19. 19.
    Revenkova, E, Eijpe, M, Heyting, C, Gross, B, Jessberger, R 2001Mol. Cell. Biol.2169846998CrossRefPubMedGoogle Scholar
  20. 20.
    Evans, GJ, Morgan, A 2003Biochem. Soc. Trans.31824827PubMedGoogle Scholar
  21. 21.
    Nebel, BR, Amarose, AP, Hacket, EM 1961Science134832833PubMedGoogle Scholar
  22. 22.
    Bellve, AR, Cavicchia, JC, Millette, CF, O’Brien, DA, Bhatnagar, YM, Dym, M 1977J. Cell Biol.746885CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Shanye Gu
    • 1
  • Jiarui Hu
    • 2
  • Ping Song
    • 1
  • Wuming Gong
    • 1
  • Ming Guo
    • 2
  1. 1.Laboratory of Molecular Genetic and Developmental Biology, College of Life ScienceWuhan UniversityWuhanChina
  2. 2.Department of Gynecology and Obstetrics, Zhongnan HospitalWuhan UniversityWuhanChina

Personalised recommendations