Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Genetic analysis and gene detection of fructan content using DArT molecular markers in spring bread wheat (Triticum aestivum L.) grain

  • 45 Accesses

Abstract

Fructans (fructo-oligosaccharides) is one of the most important carbohydrates in wheat vegetative tissues and plays a vital role in wheat grain development. One hundred and forty 'Avocet'× 'Chilero' wheat (Triticum aestivum L.) recombinant inbred lines (RILs) were phenotyped for grain fructans at Luoyang and Langzhou field sites in the 2015-2016 and 2016-2017 growing seasons and 2090 Diversity Arrays Technology (DArT) molecular marker data were used to determine genomic regions controlling fructan content. Eleven significant quantitative trait loci (QTL) for wheat fructan content were identified on chromosomes 1B, 2A, 2B, 2D, 3D, 4B, 6B, 6D, 7A, and 7B using the inclusive composite interval mapping (ICIM) method, explaining 4.08% to 27.40% of the phenotypic variance, with seven exceeding 10%. The single environment analysis identified QF.haust-6D.1 and QF.haust-7B in Luoyang, QF.haust-1B, QF.haust-2D, QF.haust-3D, and QF.haust-7B in Lanzhou, and QF.haust-1B, QF.haust-7A, and QF.haust-7B in average phenotypic performance. The multi-environment analysis identified QF.haust-2B and QF.haust-7B, which explained 21.01% and 17.39% of the phenotypic variance, respectively. The positive allele of QF.haust-2B and QF.haust-7B were all originated from Chilero. QF.haust-2B was flanked by the 3957031 and SNP1203021 markers; candidate genes for QF.haust-2B were identified by anchoring marker sequences in the IGWSC wheat genome sequence, involving a variety of biological processes, such as the regulation of ribosomes metabolism in wheat. We also constructed the physical map for wheat grain fructan QF.haust-2B and QF.haust-7B, QF.haust-7B with a physical length of 11.47 Mb between marker SNP3026392 and 3954877 and 103 genes within the segment, in which ten were related to glucose metabolism. This study provides a theoretical basis and technical support for wheat grain fructan content-related genes and molecular marker-assisted breeding.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

References

  1. Asíns MJ (2002) Present and future of quantitative trait locus analysis in plant breeding. Plant Breed 121(4):281–291

  2. Basnet BR, Singh RP, Ibrahim AMH, Herrera-Foessel SA, Huerta-Espino J, Lan C, Rudd JC (2014) Characterization of Yr54 and other genes associated with adult plant resistance to yellow rust and leaf rust in common wheat Quaiu 3. Mol Breed 33(2):385–399. https://doi.org/10.1007/s11032-013-9957-2

  3. Dong Y, Liu JD, Zhang Y, Geng HW, Rasheed A, Xiao YG, Cao SH, Fu LP, Yan J, Wen WE, Zhang Y, Jing RL, Xia XC, He ZH (2016a) Genome-wide association of stem water soluble carbohydrates in bread wheat. PLoS One 11(11):e0164293. https://doi.org/10.1371/journal.pone.0164293

  4. Dong Y, Zhang Y, Xiao YG, Yan J, Liu JD, Wen WE, Zhang Y, Jing RL, Xia XC, He ZH (2016b) Cloning of TaSST genes associated with water soluble carbohydrate content in bread wheat stems and development of a functional marker. Theor Appl Genet 129(5):1061–1070. https://doi.org/10.1007/s00122-016-2683-5

  5. Goggin DE, Setter TL (2004) Fructosyltransferase activity and fructan accumulation during development in wheat exposed to terminal drought. Funct Plant Biol 31:11–21. https://doi.org/10.1071/FP03123

  6. Guo RP, Xin ZY, Wang ZQ, Guo XY, Zhang LT, Wang JZ, Lin TB (2015) Effect of drought stress on non-structure carbohydrate metabolism of wheat and its relationship with drought resistance. Acta Agriculturae Boreali-Sinica 30(2):202–211. https://doi.org/10.7668/hbnxb.2015.02.035

  7. He XL, Wang JW, Li WX, Chen ZZ, Wu J, Zhao JX, Su JN, Wang ZH, Chen XH (2016) An intronless sucrose:fructan-6-fructosyltransferase (6-SFT) gene from Dasypyrum villosum enhances abiotic tolerance in tobacco. Biol Plant 61(2):235–245. https://doi.org/10.1007/s10535-016-0696-1

  8. Hisano H, Kanazawa A, Kawakami A, Yoshida M, Shimamoto Y, Yamada T (2004) Transgenic perennial ryegrass plants expressing wheat fructosyltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing. Plant Sci 167(4):861–868. https://doi.org/10.1016/j.plantsci.2004.05.037

  9. Huynh B-L, Palmer L, Mather DE, Wallwork H, Graham RD, Welch RM, Stangoulis JCR (2008) Genotypic variation in wheat grain fructan content revealed by a simplified HPLC method. J Cereal Sci 48(2):369–378. https://doi.org/10.1016/j.jcs.2007.10.004

  10. Kawakami A, Yoshida M (2002) Molecular characterization of sucrose:sucrose 1-fructosyltransferase and sucrose:fructan 6-fructosyltransferase associated with fructan accumulation in winter wheat during cold hardening. Biosci Biotechnol Biochem 66(11):2297–2305. https://doi.org/10.1271/bbb.66.2297

  11. Kerepesi I, Bányai-Stefanovits É, Galiba G (2002) Fructans in wheat under stress conditions. Acta Biologica Szegediensis 46(3–4):101–102,2002

  12. Khoshro HH, Taleei A, Bihamta MR, Shahbazi M, Abbasi A, Ramezanpour SS (2014) Expression analysis of the genes involved in accumulation and remobilization of assimilates in wheat stem under terminal drought stress. Plant Growth Regul 74(2):165–176. https://doi.org/10.1007/s10725-014-9908-x

  13. Kosambi DD (1943) The estimation of map distances from recombinationvalues. Ann Hum Genet 12(1):172–175

  14. Li SS (2015) Inclusive composite interval mapping of quantitative trait loci by environment interactions. M.S. Candidate, Chinese Academy of Agricultural Sciences

  15. Li H, Hearne S, Bänziger M, Li Z, Wang J (2010a) Statistical properties of QTL linkage mapping in biparental genetic populations. Heredity 105(3):257–267

  16. Li HH, Zhang LY, Wang JK (2010b) Analysis and answers to frequently asked questions in quantitative trait locus mapping. Acta Agron Sin 36(6):918–931. https://doi.org/10.3724/sp.J.1006.2010.00918

  17. Liu YQ, Huang XS (2005) Determination of fructan in garlic. Food Ferment Ind (08):84–86. https://doi.org/10.13995/j.cnki.11-1802/ts.2005.08.022

  18. Mu HR, Jiang D, Cai J, Dai TB, Cao WX (2012) Effects of shading on metabolism of fructan in winter wheat stem. Journal of Nanjing Agricultural University 35(1):1–6

  19. Peshev D, Vergauwen R, Moglia A, Hideg E, Van den Ende W (2013) Towards understanding vacuolar antioxidant mechanisms: a role for fructans? J Exp Bot 64(4):1025–1038. https://doi.org/10.1093/jxb/ers377

  20. Pillen K, Zacharias A, Léon J (2003) Advanced backcross QTL analysis in barley (Hordeum vulgare L.). Theor Appl Genet 107(2):340–352

  21. Ponce-Molina LJ, Huerta-Espino J, Singh RP, Basnet BR, Alvarado G, Randhawa MS, Lan CX, Aguilar-Rincon VH, Lobato-Ortiz R, Garcia-Zavala JJ (2018) Characterization of leaf rust and stripe rust resistance in spring wheat ‘Chilero’. Plant Dis 102:421–427. https://doi.org/10.1094/PDIS-11-16-1545-RE

  22. Savitch LV, Harney T, Huner NPA (2000) Sucrose metabolism in spring and winter wheat in response to high irradiance, cold stress and cold acclimation. Physiol Plant 108:270–278. https://doi.org/10.1034/j.1399-3054.2000.108003270.x

  23. Tognetti JA, Calderón PL, Pontis HG (1989) Fructan metabolism: reversal of cold acclimation. J Plant Physiol 134(2):232–236. https://doi.org/10.1016/s0176-1617(89)80061-6

  24. Valluru R, Van den Ende W (2008) Plant fructans in stress environments: emerging concepts and future prospects. J Exp Bot 59(11):2905–2916. https://doi.org/10.1093/jxb/ern164

  25. Van den Ende W, De Coninck B, Van Laere A (2004) Plant fructan exohydrolases: a role in signaling and defense? Trends Plant Sci 9(11):523–528. https://doi.org/10.1016/j.tplants.2004.09.008

  26. Van den Ende W, Yoshida M, Clerens S, Vergauwen R, Kawakami A (2005) Cloning, characterization and functional analysis of novel 6-kestose exohydrolases (6-KEHs) from wheat (Triticum aestivum L.). New Phytol 166(3):917–932. https://doi.org/10.1111/j.1469-8137.2005.01394.x

  27. Veenstra LD, Jannink J-L, Sorrells ME (2017) Wheat Fructans: a potential breeding target for nutritionally improved, climate-resilient varieties. Crop Sci 57(3). https://doi.org/10.2135/cropsci2016.11.0955

  28. Verspreet J, Cimini S, Vergauwen R, Dornez E, Locato V, Le Roy K, De Gara L, Van den Ende W, Delcour JA, Courtin CM (2013) Fructan metabolism in developing wheat (Triticum aestivum L.) kernels. Plant Cell Physiol 54(12):2047–2057. https://doi.org/10.1093/pcp/pct144

  29. Wang JK (2009) Inclusive composite interval mapping of quantitative trait genes. Acta Agron Sin 35(2):239–245. https://doi.org/10.3724/SP.J.1006.2009.00239

  30. Yang JC, Zhang JH, Huang ZL, Zhu QS, Wang L (2000) Remobilization of carbon reserves is improved by controlled soil-drying during grain filling of wheat. Crop Sci 40(6):1645–1655. https://doi.org/10.2135/cropsci2000.4061645x

  31. Yu HX, Xiao J, Tian JC (2012) Genetic dissection of milestone parent aimengniu and its derivatives. Sci Agric Sin 45(2):199–207. https://doi.org/10.3864/j.issn.0578-1752.2012.02.001

  32. Yue AQ, Li A, Mao XG, Chang XP, Li RZ, Jing RL (2015) Identification and development of a functional marker from 6-SFT-A2 associated with grain weight in wheat. Mol Breed 35:63. https://doi.org/10.1007/s11032-015-0266-9

  33. Yue AQ, Li A, Mao XG, Chang XP, Liu YP, Li RZ, Jing RL (2016) Sequence polymorphism and cumulative effect with 6-SFT-A2 of fructan biosynthesis gene 6-SFT-D in wheat. Acta Agron Sin 42(1):11–18. https://doi.org/10.3724/SP.J.1006.2016.00011

  34. Zhai HQ, Wang JK (2007) Applied quantitative genetics. China Agricultural Scientech Press, Beijing

  35. Zhang LY, Wang W, Liu DC, Sun JZ, Yang WL, He QC, Zhang AM (2012) Using DArt markers to identify the 1BL/1RS translocation lines among common wheat varirties in Southwest China. Journal of Triticeae Crops 32(5):832–838

  36. Zhao WC, Gao X, Dong J, BONNETT DG, L OB (2005) Dry matter, fructan and nit rogen accumulation and partitioning and their correlation with grain yield and quality in wheat. Journal of Northwest A & F University (Natural Science Edition) 33(3):43–47. https://doi.org/10.13207/j.cnki.jnwafu.2005.03.010

  37. Zhao XH, Qin Y, Jia BY, Kim S-M, Lee H-S, Eun M-Y, Kim K-M, Sohn J-K (2013) Comparion and analysis of QTLs, epistatic effects and QTL×Environment interactions for yield traits using DH and RILs populations in rice. J Integr Agric 12(2):198–208. https://doi.org/10.1016/S2095-3119(13)60219-1

Download references

Acknowledgments

We are grateful to Dr. Ravi P. Singh, Prof. Rudi Appels, Prof. Chengdao Li, Dr. Zhonghu He, and Dr. Yonggui Xiao for their critical review of this manuscript. Provision of the mapping population and DArT map by CIMMYT is also highly appreciated.

Funding

This work was financially supported by the National Key Research and Development Program of China (2018YFD0100904), the Natural Science Foundation of Henan Province (162300410077), and the International Cooperation Project of Henan Province (172102410052).

Author information

Correspondence to Chunping Wang or Caixia Lan.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Appendix

Appendix

Fig. 9
figure9figure9figure9

Phenotypic performance of two environments and average values

Fig. 10
figure10

Multi-environment QTL analysis

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Zeng, Z., Wang, C., Wang, Z. et al. Genetic analysis and gene detection of fructan content using DArT molecular markers in spring bread wheat (Triticum aestivum L.) grain. Mol Breeding 40, 23 (2020). https://doi.org/10.1007/s11032-020-1102-4

Download citation

Keywords

  • Spring bread wheat
  • Fructan content
  • DArT marker
  • QTL mapping