Mapping malting quality and yield characteristics in a north American two-rowed malting barley × wild barley advanced backcross population
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Due to the stringent quality requirements imposed by the US malting barley industry, barley breeders have been reluctant to introduce exotic germplasm into cultivar development programs. To determine whether wild barley (Hordeum vulgare ssp. spontaneum) contains favorable alleles for yield and malting quality characteristics, we mapped quantitative trait loci (QTL) for heading date, height, lodging, yield, and nine malting parameters important to the malting and brewing industry. Traits were mapped in a wild x cultivated barley BC2-derived advanced backcross mapping population. Harrington, the recurrent parent, is a North American two-rowed malting barley cultivar, and OUH602, the donor parent, is a wild barley accession that exhibits resistance to multiple barley diseases. The 98 derived lines were grown in replicated field trials at St. Paul and Crookston, MN in 2009–2011. One to four QTL were identified for each trait, for a total of 36 QTL. Trangressive segregants for increased yield were identified and four lines had higher yield than Harrington across all environments; however, for yield QTL the OUH602 alleles decreased the trait value. Wild barley alleles had both positive and negative effects on the malting traits of diastatic power, free amino nitrogen, and soluble protein. Combined with the previously identified QTL associated with resistance to fungal diseases, this population represents a rich resource for barley breeding. To facilitate future breeding and genetics studies with this population, a set of pre-introgression lines composed of 28 BC2-derived and 6 BC3-derived lines were identified that collectively contain introgressions across the entire OUH602 genome.
KeywordsWild barley Malting quality Introgression QTL Yield
The authors thank Shane Heinen for greenhouse support, and Ed Schiefelbein and Guillermo Velasquez for field plot management. We thank Dr. Shiaoman Chao (USDA-ARS, Fargo, ND) for SNP genotyping and Mr. John Barr for assistance with micro-malting and malt analyses.
Author contribution statement
GJM, KPS, and BJS designed the study. LN and PS collected experimental data. LN and YH analyzed the data. LN drafted the manuscript. All authors revised and approved the manuscript.
The study was funded by a grant to GJM from the USDA-NIFA (2011-68002-30029)
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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