Root-specific expression of rice OsHMA3 reduces shoot cadmium accumulation in transgenic tobacco
- 207 Downloads
Cadmium (Cd) is a toxic trace element released into the environment by anthropogenic activities. Its release threatens the growth of plants and contaminates the food chain. Tobacco (Nicotiana tabacum L.) is a Cd accumulator, and tobacco smoking is a major source of Cd exposure for smoking people. In the present study, we generated transgenic tobacco plants expressing the rice heavy metal P-type ATPase 3 gene (OsHMA3) in the roots under the control of a novel root-specific promoter from a tobacco root extensin-like protein-coding gene (NtREL1). Transgenic plants showed significantly reduced Cd accumulation in the shoots in both hydroponic and soil pot experiments. Analysis of Cd concentration in xylem sap showed that the transgenic plants had significantly decreased root-to-shoot translocation of Cd relative to that of wild-type plants. Moreover, a significantly lower oxidative stress level under Cd stress was observed in the shoots of transgenic plants than in wild-type shoots. These results suggest that OsHMA3 can be expressed in tobacco plants and may be useful for developing tobacco varieties with a reduced capacity to accumulate Cd in the shoots, potentially reducing the risk of Cd exposure for smoking people.
KeywordsCadmium OsHMA3 Root-specific expression Tobacco Heavy metals
The study was funded by the Science Research Project of Hunan Tobacco Company (No. CYKJ2014-03) and the Fundamental Research Funds for the Central Universities (KYZ201873).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Gallois P, Marinho P (1995) Leaf disk transformation using Agrobacterium tumefaciens-expression of heterologous genes in tobacco. Methods Mol Biol (Clifton, NJ) 49:39–48Google Scholar
- Geiss O, Kotzias D (2007) Tobacco, cigarettes and cigarette smoke an overview. Institute for Health and Consumer Protection, European CommissionGoogle Scholar
- Han Y, Sa G, Sun J, Shen Z, Zhao R, Ding M, Deng S, Lu Y, Zhang Y, Shen X, Chen S (2014) Overexpression of Populus euphratica xyloglucan endotransglucosylase/hydrolase gene confers enhanced cadmium tolerance by the restriction of root cadmium uptake in transgenic tobacco. Environ Exp Bot 100:74–83CrossRefGoogle Scholar
- Lugon-Moulin N, Zhang M, Gadani F, Rossi L, Koller D, Krauss M, Wagner GJ (2004) Critical review of the science and options for reducing cadmium in tobacco (Nicotiana tabacum L.) and other plants. In: Advances in agronomy, vol 83, pp 111–180Google Scholar
- Miyadate H, Adachi S, Hiraizumi A, Tezuka K, Nakazawa N, Kawamoto T, Katou K, Kodama I, Sakurai K, Takahashi H, Satoh-Nagasawa N, Watanabe A, Fujimura T, Akagi H (2011) OsHMA3, a P-1B-type of ATPase affects root-to-shoot cadmium translocation in rice by mediating efflux into vacuoles. New Phytol 189:190–199CrossRefGoogle Scholar
- Smeets K, Opdenakker K, Remans T, Van Sanden S, Van Belleghem F, Semane B, Horemans N, Guisez Y, Vangronsveld J, Cuypers A (2009) Oxidative stress-related responses at transcriptional and enzymatic levels after exposure to Cd or Cu in a multipollution context. J Plant Physiol 166:1982–1992CrossRefGoogle Scholar