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Molecular Breeding

, 39:26 | Cite as

Characterization, validation, and cross-species transferability of newly developed EST-SSR markers and their application for genetic evaluation in crape myrtle (Lagerstroemia spp)

  • Yuanjun Ye
  • Lu Feng
  • Xiaohan Liang
  • Tingting Liu
  • Ming Cai
  • Tangren Cheng
  • Jia Wang
  • Qixiang Zhang
  • Huitang PanEmail author
Short Communication
  • 3 Downloads

Abstract

Crape myrtle is an important ornamental woody plant, due to its long-lasting midsummer bloom and rich color. However, limited molecular markers on this species hinder the breeding and genetic studies. In this work, 8652 EST-SSRs were identified from L. indica transcriptome data. Di-nucleotide repeats (57.1%) were the most abundant type followed by tri-, tetra-, penta-, and hexa-nucleotide repeats, with the AG/CT motif occurring most frequently. Of the 1200 synthesized primer pairs, 761 EST-SSRs (63.4%) were successfully amplified and 245 EST-SSRs (20.4%) showed polymorphic. High cross-species transferabilities of these markers were observed except in L. speciosa (26.7%). The polymorphic information content (PIC) for each locus ranged from 0.210 to 0.813 with a mean of 0.589, suggesting a high level of informativeness. Using 30 polymorphic EST-SSRs, structure and cluster analyses roughly divided the 73 genotypes into three major groups with some admixtures. This work contributes to the better understanding of the genetic diversity and germplasm resources conservation in Lagerstroemia species. Thus, the newly developed EST-SSRs will provide a valuable tool for genetic analysis, linkage map construction, and marker-assisted selection breeding in crape myrtle.

Keywords

Lagerstroemia Transcriptome EST-SSR markers Cross-transferability Genetic diversity 

Notes

Funding information

This work was financially supported by the National Natural Science Foundation of China (no. 31470695) and the 12th Five Years Key Programs for Science and Technology Development of China (no. 2013BAD01B07).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The authors declare that the experiments comply with the current laws of China.

Supplementary material

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Yuanjun Ye
    • 1
  • Lu Feng
    • 1
  • Xiaohan Liang
    • 1
  • Tingting Liu
    • 1
  • Ming Cai
    • 1
  • Tangren Cheng
    • 1
  • Jia Wang
    • 1
  • Qixiang Zhang
    • 1
  • Huitang Pan
    • 1
    Email author
  1. 1.Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education and College of Landscape ArchitectureBeijing Forestry UniversityBeijingChina

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