Molecular mapping of the downy mildew and rust resistance genes in a sunflower germplasm line TX16R
- 12 Downloads
The sunflower germplasm line TX16R is resistant to Plasmopara halstedii (causal agent of sunflower downy mildew) and Puccinia helianthi (causal agent of sunflower rust), which are two destructive foliar diseases in sunflower production worldwide. This study reports the mapping of the downy mildew and rust resistance genes Pl33 and R16 from TX16R, respectively. Progeny testing of test crosses for downy mildew resistance suggested that Pl33 localizes to linkage group (LG) 4 of the sunflower genome. Molecular mapping of Pl33 using simple sequence repeat (SSR) and single-nucleotide polymorphism (SNP) markers identified Pl33 cosegregating with ORS644, ORS963, SFW04901, and SFW04052, and linking to two SNPs, NSA_006089 and NSA_008496, at a genetic distance of 0.2 cM on the proximal side. Bulked segregant analysis using SSR and EST-SSR markers from LGs previously reported for rust genes identified polymorphic SSR markers associated with rust resistance on LG13. R16 was mapped between SFW08875 and SFW04317 on LG13, with a genetic distance of 1.8 and 1.1 cM, respectively. The 15 linked markers span a genetic distance of 27.4 cM in LG13. The cosegregating or closely linked markers to the two resistance genes will facilitate marker-assisted selection (MAS) and gene pyramiding, and will further assist in identifying genes responsible for DM and rust resistance.
KeywordsSunflower Downy mildew Rust Resistance SSR SNP marker
The authors are very grateful to Lisa Brown for the technical assistance in this study. We thank Ms. Puying Zheng for her help in molecular marker genotyping and Dr. Fang Wei for his help in phenotyping to rust. We appreciate Drs. Yunming Long and Zahirul Talukder for their help during the SNP primer design. This project was supported by the USDA-ARS CRIS Project No. 3060-21000-043-00D. The mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer.
Conceived and designed the experiments: CCJ, ZL. Performed the experiments: ZL, LZ, GJM, CCJ, GS. Analyzed the data: ZL, GJM, LLQ. Wrote the paper: ZL, LLQ. Commented on the manuscript before submission: CCJ, GJM, GS.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The experiments were performed in compliment with the current laws of the USA.
- Bachlava E, Radwan OE, Abratti G, Tang S, Gao W, Heesacker AF, Bazzalo ME, Zambelli A, Leon AJ, Knapp SJ (2011) Downy mildew (Pl 8 and Pl 14) and rust (R Adv) resistance genes reside in close proximity to tandemly duplicated clusters of non-TIR-like NBS-LRR-encoding genes on sunflower chromosomes 1 and 13. Theor Appl Genet 122:1211–1221CrossRefGoogle Scholar
- de Romano AB, Romano C, Bulos M, Altieri E, Sala C (2010) A new gene for resistance to downy mildew in sunflower. In: proceedings of Int symposium “sunflower breeding on resistance to diseases”, Krasnodar, Russia, June 23–24, 2010Google Scholar
- Gedil MA, Slabaugh MB, Berry S, Segers B, Peleman J, Michelmore R, Miller JF, Gulya T, Knapp SJ (2001) Candidate disease resistance genes in sunflower cloned using conserved nucleotide binding site motifs: genetic mapping and linkage to downy mildew resistance gene Pl1. Genome 44:205–212CrossRefGoogle Scholar
- Gilley MA, Markell SG, Gulya TJ, Misar CG (2016) Prevalence and virulence of Plasmopara halstedii (downy mildew) in sunflowers. Available at http://www.sunflowernsa.com/uploads/research/1277/Prevalence.Downey_Gilley.etal_2016.rev.pdf. Accessed 4 June 2018
- Gulya TJ (1985) Registration of five disease-resistant sunflower germplasms. Crop Sci 25:719–720Google Scholar
- Gulya TJ (2006) The sunflower rust situation: Current races in the northern and central Great Plains, and resistance in oilseed and confection hybrids Available at http://www.sunflowernsa.com/research/researchworkshop/documents/Gulya_Rust_06.pdf. Accessed 4 June 2018
- Gulya TJ, Draper M, Harbour J, Holen C, Knodel J, Lamey A, Mason P (1999) Metalaxyl resistance in sunflower downy mildew in North America. Proc. 21st sunflower res. Workshop. p. 118-123, January 14–15Google Scholar
- Gulya TJ, Markell S (2009) Sunflower rust status – 2008 race frequency across the Midwest and resistance among commercial hybrids. Available at http://www.sunflowernsa.com/uploads/Gulya_RustStatus_09.pdf. Accessed 4 June 2018
- Gulya TJ, Markell S, McMullen M, Harveson B, Osborne L (2011) New virulent races of downy mildew: distribution, status of DM resistant hybrids, and USDA sources of resistance. Available at http://www.sunflowernsa.com/uploads/resources/575/gulya_virulentracesdownymildew.pdf. Accessed 4 June 2018
- Livaja M, Wang Y, Wieckhorst S, Haseneyer G, Hahn V, Knapp SJ, Taudien S, Schön CC, Bauer E (2013) BSTA: a targeted approach combines bulked segregant analysis with next-generation sequencing and de novo transcriptome assembly for SNP discovery in sunflower. BMC Genomics 14:628CrossRefGoogle Scholar
- Pecrix Y, Buendia L, Penouilh-Suzette C, Maréchaux M, Legrand L, Bouchez Q, Rengel D, Gouzy J, Cottret L, Vear F, Godiard L (2018a) Sunflower resistance to multiple downy mildew pathotypes revealed by recognition of conserved effectors of the oomycete Plasmopara halstedii. The Plant Journal doi. https://doi.org/10.1111/tpj.14157
- Roeckel-Drevet P, Gagne G, Mouzeyar S, Gentzbittel L, Philippon J, Nicolas P, de Labrouhe DT, Vear F (1996) Colocation of downy mildew (Plasmopara halstedii) resistance genes in sunflower (Helianthus annuus L.). Euphytica 91:225–228Google Scholar
- Talukder ZI, Gong L, Hulke BS, Pegadaraju V, Song QJ, Schultz Q, Qi LL (2014) A high-density SNP map of sunflower derived from RAD-sequencing facilitating fine-mapping of the rust resistance gene R 12. PLoS One 9(7):e98628. https://doi.org/10.1371/journal.pone.0098628 CrossRefPubMedPubMedCentralGoogle Scholar
- Van Ooijen JW (2006) JoinMap® 4, software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, WageningenGoogle Scholar
- Vincourt P, As-sadi F, Bordat A, Langlade NB, Gouzy J, Pouilly N, Lippi Y, Serre F, Godiard L, Tourvieille de Labrouhe D, Vear F (2012) Consensus mapping of major resistance genes and independent QTL for quantitative resistance to sunflower downy mildew. Theor Appl Genet 125:909–920CrossRefGoogle Scholar
- Viranyi F, Gulya TJ, Tourieille DL (2015) Recent changes in the pathogenic variability of Plasmopara halstedii (sunflower downy mildew) populations from different continents. Helia 0. https://doi.org/10.1515/helia-2015-0009
- Wei F, Gobelman-Werner K, Morroll SM, Kurth J, Mao L, Wing R, Leister D, Schulze-Lefert P, Wise RP (1999) The Mla (powdery mildew) resistance cluster is associated with three NBS-LRR gene families and suppressed recombination within a 240-kb DNA interval on chromosome 5S (1HS) of barley. Genetics 153:1929–1948PubMedPubMedCentralGoogle Scholar
- Zhang ZW, Ma GJ, Zhao J, Markell SJ, Qi LL (2017) Discovery and introgression of the wild sunflower-derived novel downy mildew resistance gene Pl 19 in confection sunflower (Helianthus annuus L.) Theor Appl genet 130:29–39Google Scholar