Development of simple sequence repeat (SSR) markers in Medicago ruthenica and their application for evaluating outcrossing fertility under open-pollination conditions
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Medicago ruthenica is a perennial leguminous plant with great economic value for forage production and soil conservation in northern China. Simple sequence repeat (SSR) markers are a major molecular tool for genetic and genomic research that have been extensively developed and used in major crops. However, few are available in M. ruthenica, an important cool-season forage species in China. Using transcriptome data, we developed 213 polymorphic SSR markers. Additionally, we used these markers to quantify selfing and outcrossing rates of M. ruthenica plants under field conditions. The outcrossing rate was calculated to be 75.9%. The first large set of codominant markers developed for M. ruthenica and knowledge of its outcrossing behavior should be highly valuable for molecular and traditional biological research.
KeywordsSimple sequence repeat (SSR) Medicago ruthenica Outcrossing fertility
The authors would like to acknowledge Yanqi Wu from Oklahoma State University for kindly providing suggestions. This work has been supported by a grant from the National Natural Science Foundation of China (31601998), Ministry of Agriculture Crop Germplasm Resources Protection and Utilization Project (2018NWB037), and the Central Non-profit Research Institutes Fundamental Research Funds of China (1610332017003).
J.L. and H.L. conceived and designed the experiment. Z.L. supervised the entire study. E.C. and Z.D. performed the experiments. E.C., F.H., and L.L. analyzed the data. W.S. and F.M. prepared the materials. J.L. wrote the paper. All authors read and approved the final manuscript.
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