Molecular Breeding

, 38:130 | Cite as

Assay development and marker validation for marker assisted selection of Fusarium oxysporum f. sp. niveum race 1 in watermelon

  • Leigh Ann FallEmail author
  • Josh Clevenger
  • Cecilia McGregor


Fusarium wilt, Fusarium oxysporum f. sp. niveum (FON), of watermelon (Citrullus lanatus) is a fungal pathogen that causes significant yield losses in the US watermelon industry. FON damages watermelon through invasion of the root system and remains a difficult pathogen to manage due to its long-lasting survival spores which persist in the soil. Chemical control options for this pathogen are lacking, making development of genetic resistance the best option. There are four known races of FON (0, 1, 2, and 3) which are distinguished based on their pathogenicity of differential cultivars. Most modern cultivar releases have FON race 1 (FON-1) resistance, which has been mapped on the end of chromosome 1. Application of marker assisted selection (MAS) would improve the efficiency of FON-1 resistance breeding. In order to identify markers for selection in the FON-1 region, the QTL-seq method was utilized on an F2 population segregating for FON-1 resistance. Single nucleotide polymorphism (SNP) markers in the region were developed into Kompetitive allele-specific PCR (KASP™) assays and tested for trait association on the segregating F2:3 population. Marker validation was done using an F2 population from a cross between FON-1 susceptible “New Hampshire Midget” and FON-1-resistant “Calhoun Gray.” Further validation on a panel of susceptible and resistant cultivars and Plant Introductions identified SNP marker UGA1_502161 as a useful marker for selection of FON-1 resistance from Calhoun Gray.


Citrullus lanatus MAS SNP QTL-seq Fusarium wilt QTL 



This work was in part supported by the United States Department of Agriculture Specialty Crop Research Initiative Award No. 2014-51181-22471.

Supplementary material

11032_2018_890_MOESM1_ESM.docx (96 kb)
ESM 1 (DOCX 96 kb)
11032_2018_890_MOESM2_ESM.docx (33 kb)
Online Resource 1 (DOCX 32 kb)


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Leigh Ann Fall
    • 1
    Email author
  • Josh Clevenger
    • 2
  • Cecilia McGregor
    • 1
  1. 1.Institute of Plant Breeding, Genetics & GenomicsUniversity of GeorgiaAthensUSA
  2. 2.Institute of Plant Breeding, Genetics & GenomicsUniversity of GeorgiaAthensUSA

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