Molecular Breeding

, 38:138 | Cite as

Development of SSR markers linked to QTL reducing leaf hair density and grapevine downy mildew resistance in Vitis vinifera

  • Atsushi KonoEmail author
  • Yusuke Ban
  • Nobuhito Mitani
  • Hiroshi Fujii
  • Shusei Sato
  • Koichi Suzaki
  • Akifumi Azuma
  • Noriyuki Onoue
  • Akihiko Sato


Dense leaf hairs of grapevines have been known to act as a preexisting defense structure for preventing the incidence of grapevine downy mildew, because the pathogen, Plasmopara viticola, needs water to invade grapevines, and water is repelled by a hydrophobic surface due to dense leaf hairs. In the present study, we performed regression analyses of downy mildew resistance with leaf hair density using hybrids of Vitis labrusca origin and confirmed the effect of leaf hairs. Reducing the repelling effect of leaf hairs by detergent application canceled the effect of leaf hair, which confirmed the hypothesis. Thereafter, based on QTL analyses in the population of V. vinifera ‘Muscat of Alexandria’ × the interspecific hybrid ‘Campbell Early,’ we identified a major locus in linkage group (LG) 5 of ‘Muscat of Alexandria’ controlling leaf hair density. This locus was previously reported as a small effect QTL for downy mildew resistance, however we found that the locus had high LOD scores explaining 71.9%–78.5% of the phenotypic variance of leaf hairs. Moreover, this locus was detected as a QTL for downy mildew resistance. The effect of this locus was confirmed in two other hybrid populations. Finally, we could successfully identify three traditional V. vinifera table grapes ‘Muscat of Alexandria,’ ‘Katta Kurgan,’ and ‘Parkent’ as the origin of the allele linked to hairlessness by defining the SSR haplotypes. The use of this locus for marker-assisted selections would be a promising strategy for producing grapevines with resistance by preexisting defense structure.


DNA marker Grapevine downy mildew Leaf hairs Preexisting defense structure Vitis vinifera Vitis labrusca 



We thank Takeshi Hayashi (NARO, Tsukuba, Japan) for support in Pop AC mapping and manuscript revision, Ryosuke Mochioka (Kagawa University, Kagawa, Japan) and Hino Motosugi (Kyoto Prefectural University, Kyoto, Japan) for providing Japanese wild Vitis species, Natsumaro Kutsuna (LPixel Inc. Tokyo, Japan) for support in imaging analysis, and Technical Support Center Operations Unit 1 in Akitsu for their technical support in vineyards. We are grateful to Mirai Nakahara, Miho Kohata, Tamami Nakasumi, and Sumie Kurokawa (NARO, Hiroshima, Japan) for technical assistance, and to Takao Ito (NARO, Hiroshima, Japan) for critical reading of the manuscript.

Author’s contributions

A. K. wrote the manuscript. A. K., Y. B., K. S., A. A., N. O., and A. S. designed the experiments. A. A. contributed to the experiments using ‘Pinot Meunier.’ A. K. and A. S. contributed to statistical data analyses. A. K. performed the experiments. A. K., Y. B., N. M., and S. S. genotyped Pop AC. Y. B. and N. M. developed populations. H. F. extracted SSRs from the grapevine reference genome.

Funding information

This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation, HOR-2006).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11032_2018_889_MOESM1_ESM.docx (66 kb)
Supplementary Tables (DOCX 66.4 kb)
11032_2018_889_MOESM2_ESM.docx (5.6 mb)
Supplementary Figs. 1 to 6 (DOCX 5.63 mb)
11032_2018_889_MOESM3_ESM.pdf (244 kb)
Supplementary Fig. 7 (PDF 244 kb)
11032_2018_889_MOESM4_ESM.docx (354 kb)
Supplementary Fig. 8 (DOCX 353 kb)


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Division of Grape and Persimmon Research, Institute of Fruit Tree and Tea ScienceNational Agriculture and Food Research Organization (NARO)HigashihiroshimaJapan
  2. 2.Division of Lowland Crop ResearchWestern Region Agricultural Research Center, NAROFukuyamaJapan
  3. 3.Division of Fruit Production and Postharvest ScienceInstitute of Fruit Tree and Tea Science, NAROTsukubaJapan
  4. 4.Division of Citrus ResearchInstitute of Fruit Tree and Tea Science, NAROShizuokaJapan
  5. 5.Graduate School of Life SciencesTohoku UniversitySendaiJapan

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