Molecular Breeding

, 37:70 | Cite as

Validation of molecular markers associated with perpetual flowering in Octoploid Fragaria germplasm

  • Natalia R. Salinas
  • Jason D. Zurn
  • Megan Mathey
  • Sonali Mookerjee
  • Beatrice Denoyes
  • Justine Perrotte
  • Aline Potier
  • Chad E. Finn
  • James F. Hancock
  • Philip Stewart
  • Nahla V. Bassil
Article
First Online:
Received:
Accepted:

Abstract

Perpetual-flowering (PF) is a highly desirable trait within cultivated strawberries (Fragaria ×ananassa) for the commercial and home garden markets. The most widely used source of the PF trait was originally introgressed from a wild F. virginiana subsp. glauca accession collected in the Wasatch Mountains near Salt Lake City, UT in 1955. This source is conferred by a single dominant QTL, FaPFRU, and was recently identified in multiple bi-parental populations. Multiple markers have been proposed as diagnostic tests for marker-assisted selection (MAS). These markers were proposed after looking at a relatively small sample of germplasm. To identify the best diagnostic testing procedure for MAS, the markers were evaluated individually and in combination on a training set of cultivars with known genotypes and the best test was used to determine the distribution of the FaPFRU source of PF within a large sample of octoploid Fragaria germplasm. Of the tests evaluated, the microsatellite marker Bx215 alone was found to have the best diagnostic ability for MAS with an accuracy of 93.1% in controlled conditions. When utilizing the test on 390 F. ×ananassa accessions, 164 accessions were identified to likely have the FaPFRU locus. Nine octoploid Fragaria accessions were PF and did not have this marker, indicating possible recombination events or potentially novel sources of the PF trait. Future work will be needed to dissect the PF trait in these nine individuals.

Keywords

RosBREED DNA informed breeding Day-neutral Everbearing Remontancy 
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Notes

Acknowledgements

The authors would like to thank Pete Callow, Ted Mackey, April Nyberg, Madeline Abrams, and Hannah DeVyldere for their technical support. This research was funded through the USDA’s National Institute of Food and Agriculture—Specialty Crop Research Initiative project, “RosBREED: Enabling Marker-Assisted Breeding in Rosaceae” (2009-51181-05808) and “RosBREED: Combining Disease Resistance and Horticultural Quality in New Rosaceous Cultivars” (2014-51181-22378).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

11032_2017_672_MOESM1_ESM.xlsx (33 kb)
ESM 1 (XLSX 33 kb)

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Copyright information

© © Springer Science+Business Media Dordrecht (outside the USA) 2017

Authors and Affiliations

  • Natalia R. Salinas
    • 1
  • Jason D. Zurn
    • 2
  • Megan Mathey
    • 3
  • Sonali Mookerjee
    • 4
  • Beatrice Denoyes
    • 5
  • Justine Perrotte
    • 6
  • Aline Potier
    • 5
  • Chad E. Finn
    • 7
  • James F. Hancock
    • 4
  • Philip Stewart
    • 8
  • Nahla V. Bassil
    • 2
  1. 1.Department of HorticultureOregon State UniversityCorvallisUSA
  2. 2.USDA-ARS National Clonal Germplasm RepositoryCorvallisUSA
  3. 3.Spring Meadow Nursery Inc.Grand HavenUSA
  4. 4.Department of HorticultureMichigan State UniversityEast LansingUSA
  5. 5.UMR 1332 B.P. INRAUniversity of BordeauxVillenave d’OrnonFrance
  6. 6.CIREF Création Variétale Fraises Fruits RougesDouvilleFrance
  7. 7.USDA-ARS Horticultural Crops Research UnitCorvallisUSA
  8. 8.Driscoll Strawberry AssociatesWatsonvilleUSA

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