Molecular Breeding

, 36:137 | Cite as

Physical mapping of NBS-coding resistance genes to the Me-gene cluster on chromosome P9 reveals markers tightly linked to the N gene for root-knot nematode resistance in pepper

  • Ibrahim Celik
  • Mehmet Ali Sogut
  • Ercan Ozkaynak
  • Sami Doganlar
  • Anne FraryEmail author


Natural root-knot nematode resistance genes are unique resources to control this major pest in pepper (Capsicum annuum). Although four genes (Me1, Me3, Me7 and N) conferring broad-spectrum resistance were mapped to a cluster in a 28-cm interval on chromosome P9, limited markers targeting this region were available. In the present study, the Me-gene cluster was structurally annotated for resistance genes to develop markers targeting the N gene. As a result, the Me-gene cluster (4.07 Mb in size) was found to contain three resistance gene hotspots. In addition, a SSR maker tightly linked to the N gene (0.8 cM away) was developed for marker-assisted selection in pepper.


Capsicum annuum Genome annotation Meloidogyne spp. Marker-assisted selection (MAS) 



This study was supported by grant 110O675 from the Scientific and Technological Research Council of Turkey (TUBITAK).

Authors contribution

IC analyzed all data, performed all bioinformatics analysis and laboratory experiments and drafted manuscript; MAS performed all nematode tests; EO developed mapping populations; SD designed the study; AF designed the study, received funding and revised manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

11032_2016_560_MOESM1_ESM.docx (104 kb)
Supplementary material 1 (DOCX 104 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Ibrahim Celik
    • 1
  • Mehmet Ali Sogut
    • 2
  • Ercan Ozkaynak
    • 3
  • Sami Doganlar
    • 1
  • Anne Frary
    • 1
    Email author
  1. 1.Department of Molecular Biology and GeneticsIzmir Institute of TechnologyUrla, IzmirTurkey
  2. 2.Department of Plant ProtectionSuleyman Demirel UniversityIspartaTurkey
  3. 3.Yuksel Seeds Ltd.Kiziltoprak, AntalyaTurkey

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