Molecular Breeding

, 36:129 | Cite as

Genetic analysis of grain and malt quality in an elite barley population

  • Suong T. Cu
  • Timothy J. March
  • Susan Stewart
  • Sophia Degner
  • Stewart Coventry
  • Amanda Box
  • Doug Stewart
  • Birgitte Skadhauge
  • Rachel A. Burton
  • Geoffrey B. Fincher
  • Jason Eglinton
Article

Abstract

Quantitative trait loci (QTLs) associated with grain weight, grain width, kernel hardness and malting quality were mapped in a doubled haploid population derived from two elite Australian malting barley varieties, Navigator and Admiral. A total of 30 QTLs for grain weight, grain width and kernel hardness were identified in three environments, and 63 QTLs were identified for ten malting quality traits in two environments. Three malting quality traits, namely β-amylase, diastatic power and apparent attenuation limit, were mainly controlled by a QTL linked to the Bmy1 gene at the distal end of chromosome 4H encoding a β-amylase enzyme. Six other malting quality traits, namely α-amylase, soluble protein, Kolbach index, free amino-acid nitrogen, wort β-glucan and viscosity, had coincident QTL clustered on chromosomes 1HS, 4HS, 7HS and 7HL, which demonstrated the interdependence of these traits. There was a strong association between these malt quality QTL clusters on chromosomes 1HS and 7HL and the major QTL for kernel hardness, suggesting that the use of this trait to enable early selection for malting quality in breeding programs would be feasible. In contrast, the majority of QTLs for hot-water extract were not coincident with those identified for other malt quality traits, which suggested differences in the mechanism controlling this trait. Novel QTLs have been identified for kernel hardness on chromosomes 2HL and 7HL, hot-water extract on 7HL and wort β-glucan on 6HL, and the resulting markers may be useful for marker-assisted selection in breeding programs.

Keywords

Barley breeding Kernel hardness Malting quality Marker-assisted selection Quantitative trait loci 

Supplementary material

11032_2016_554_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 12 kb)
11032_2016_554_MOESM2_ESM.xlsx (2.3 mb)
Supplementary material 2 (XLSX 2370 kb)

References

  1. Arends D, Prins P, Jansen RC, Broman KW (2010) R/qtl: high-throughput multiple QTL mapping. Bioinformatics 26:2990–2992CrossRefPubMedPubMedCentralGoogle Scholar
  2. Bamforth C (2003) Barley and malt starch in brewing: a general review. Tech Q Master Brew Assoc Am 40:89–97Google Scholar
  3. Bamforth CW (2009) Current perspectives on the role of enzymes in brewing. J Cereal Sci 50:353–357CrossRefGoogle Scholar
  4. Barr A, Karakousis A, Lance R, Logue S, Manning S, Chalmers K, Kretschmer J, Boyd W, Collins H, Roumeliotis S (2003) Mapping and QTL analysis of the barley population Chebec × Harrington. Crop Pasture Sci 54:1125–1130CrossRefGoogle Scholar
  5. Bauer AM, Hoti F, Von Korff M, Pillen K, Léon J, Sillanpää M (2009) Advanced backcross-QTL analysis in spring barley (H. vulgare ssp. spontaneum) comparing a REML versus a Bayesian model in multi-environmental field trials. Theor Appl Genet 119:105–123CrossRefPubMedPubMedCentralGoogle Scholar
  6. Broman KW, Wu H, Sen Ś, Churchill GA (2003) R/qtl: QTL mapping in experimental crosses. Bioinformatics 19:889–890CrossRefPubMedGoogle Scholar
  7. Burton RA, Jobling SA, Harvey AJ, Shirley NJ, Mather DE, Bacic A, Fincher GB (2008) The genetics and transcriptional profiles of the cellulose synthase-like HvCslF gene family in barley. Plant Physiol 146:1821–1833CrossRefPubMedPubMedCentralGoogle Scholar
  8. Camm J, Ellis R, Morrison W (1990) Milling energy. An investigation into the biochemical basis of hardness in cereals. Asp Appl Biol 25:121–130Google Scholar
  9. Celus I, Brijs K, Delcour JA (2006) The effects of malting and mashing on barley protein extractability. J Cereal Sci 44:203–211CrossRefGoogle Scholar
  10. Collins H, Barr A, Lim P, Cullis B (2007) QTL analysis of malting quality traits in two barley populations. Aust J Agric Res 58:858–866CrossRefGoogle Scholar
  11. Coventry S, Collins H, Barr A, Jefferies S, Chalmers K, Logue S, Langridge P (2003) Use of putative QTLs and structural genes in marker assisted selection for diastatic power in malting barley (Hordeum vulgare L.). Crop Pasture Sci 54:1241–1250CrossRefGoogle Scholar
  12. Cu S, Collins HM, Betts NS, March TJ, Janusz A, Stewart DC, Skadhauge B, Eglinton J, Kyriacou B, Little A (2015) Water uptake in barley grain: Physiology; genetics and industrial applications. Plant Sci 242:260–269CrossRefPubMedGoogle Scholar
  13. De Villiers O, Maree P, Laubscher E (1988) Effect of time and rate of nitrogen application on the malting quality of barley. S Afr J Plant Soil 5:134–136CrossRefGoogle Scholar
  14. Delcour JA, Verschaeve SG (1987) Malt diastatic activity. Part i. The EBC determination of diastatic power: the underestimation of the release of reducing sugars by iodometric titration and the chromogen p-hydroxybenzoic acid hydrazide as alternative method. J Inst Brew 93:121–124CrossRefGoogle Scholar
  15. Eagles H, Bedggood A, Panozzo J, Martin P (1995) Cultivar and environmental effects on malting quality in barley. Crop Pasture Sci 46:831–844CrossRefGoogle Scholar
  16. Eglinton JK, Langridge P, Evans DE (1998) Thermostability variation in alleles of barley β-amylase. J Cereal Sci 28:301–309CrossRefGoogle Scholar
  17. Evans DE, Collins HM, Eglinton JK, Wilhelmson A (2005) Assessing the impact of the level of diastatic power enzymes and their thermostability on the hydrolysis of starch during wort production to predict malt fermentability. J Am Soc Brew Chem 63:195–198Google Scholar
  18. Evans E, Li C, Eglinton JK (2008) Improved prediction of malt fermentability by measurement of the diastatic power enzymes α-amylase, β-amylase, and limit dextrinase: I. Survey of the levels of diastatic power enzymes in commercial malts. J Am Soc Brew Chem 66:223–232Google Scholar
  19. Faure S, Higgins J, Turner A, Laurie DA (2007) The FLOWERING LOCUS T-like gene family in barley (Hordeum vulgare). Genetics 176:599–609CrossRefPubMedPubMedCentralGoogle Scholar
  20. Frydenberg O, Nielsen G (1965) Amylase isozymes in germinating barley seeds. Hereditas 54:123–139CrossRefGoogle Scholar
  21. Gamlath J, Aldred G, Panozzo J (2008) Barley (1 → 3; 1 → 4)-β-glucan and arabinoxylan content are related to kernel hardness and water uptake. J Cereal Sci 47:365–371CrossRefGoogle Scholar
  22. Gao W, Clancy J, Han F, Jones B, Budde A, Wesenberg D, Kleinhofs A, Ullrich S (2004) Fine mapping of a malting-quality QTL complex near the chromosome 4H S telomere in barley. Theor Appl Genet 109:750–760CrossRefPubMedGoogle Scholar
  23. Guidet F, Rogowsky P, Taylor C, Song W, Langridge P (1991) Cloning and characterisation of a new rye-specific repeated sequence. Genome 34:81–87CrossRefGoogle Scholar
  24. Han F, Ullrich S, Chirat S, Menteur S, Jestin L, Sarrafi A, Hayes P, Jones B, Blake T, Wesenberg D (1995) Mapping of β-glucan content and β-glucanase activity loci in barley grain and malt. Theor Appl Genet 91:921–927PubMedGoogle Scholar
  25. Han F, Ullrich S, Kleinhofs A, Jones B, Hayes P, Wesenberg D (1997) Fine structure mapping of the barley chromosome-1 centromere region containing malting-quality QTLs. Theor Appl Genet 95:903–910CrossRefGoogle Scholar
  26. Han F, Clancy JA, Jones BL, Wesenberg DM, Kleinhofs A, Ullrich SE (2004) Dissection of a malting quality QTL region on chromosome 1 (7H) of barley. Mol Breed 14:339–347CrossRefGoogle Scholar
  27. Hayes PM, Liu BH, Knapp SJ, Chen F, Jones B, Blake T, Franckowiak J, Rasmusson D, Sorrells M, Ullrich SE, Wesenberg D, Kleinhofs A (1993) Quantitative trait locus effects and environmental interaction in a sample of North American barley germ plasm. Theor Appl Genet 87:392–401CrossRefPubMedGoogle Scholar
  28. Hayes P, Castro A, Marquez-Cedillo L, Corey A, Henson C, Jones B, Kling J, Mather D, Matus I, Rossi C (2000) A summary of published barley QTL reports. http://www.barleyworldorg/northamericanbarley/qtlsummaryphp
  29. Hayes PM, Castro A, Marquez-Cedillo L, Corey A, Henson C, Jones BL, Kling J, Mather D, Matus I, Rossi C (2003) Genetic diversity for quantitatively inherited agronomic and malting quality traits. In: von Bothmer R, Sato K, Knuffer H, van Hintum T (eds) Diversity in barley (Hordeum vulgare). Elsevier Science B.V, Amsterdam, pp 201–226CrossRefGoogle Scholar
  30. Henry R, Cowe I (1990) Factors influencing the hardness (milling energy) and malting quality of barley. J Inst Brew 96:135–136CrossRefGoogle Scholar
  31. Islamovic E, Obert DE, Budde AD, Schmitt M, Brunick Ii R, Kilian A, Chao S, Lazo GR, Marshall JM, Jellen EN (2014) Quantitative trait loci of barley malting quality trait components in the Stellar/01Ab8219 mapping population. Mol Breed 34:59–73CrossRefGoogle Scholar
  32. Karakousis A, Barr A, Kretschmer J, Manning S, Logue S, Roumeliotis S, Collins H, Chalmers K, Li C, Lance R (2003) Mapping and QTL analysis of the barley population Galleon × Haruna Nijo. Crop Pasture Sci 54:1131–1135CrossRefGoogle Scholar
  33. Li JZ, Huang XQ, Heinrichs F, Ganal MW, Röder MS (2005) Analysis of QTLs for yield, yield components, and malting quality in a BC 3-DH population of spring barley. Theor Appl Genet 110:356–363CrossRefPubMedGoogle Scholar
  34. Li C, Cakir M, Lance R (2010) Genetic Improvement of malting quality through conventional breeding and marker-assisted Selection. In: Zhang G, Li C (eds) Genetics and improvement of barley malt quality. Springer, Berlin, pp 260–292Google Scholar
  35. Lu F, Lipka AE, Glaubitz J, Elshire R, Cherney JH, Casler MD, Buckler ES, Costich DE (2013) Switchgrass genomic diversity, ploidy, and evolution: novel insights from a network-based SNP discovery protocol. PLoS Genet 9:e1003215CrossRefPubMedPubMedCentralGoogle Scholar
  36. Marquez-Cedillo L, Hayes P, Jones B, Kleinhofs A, Legge W, Rossnagel B, Sato K, Ullrich S, Wesenberg D (2000) QTL analysis of malting quality in barley based on the doubled-haploid progeny of two elite North American varieties representing different germplasm groups. Theor Appl Genet 101:173–184CrossRefGoogle Scholar
  37. Mather D, Tinker N, LaBerge D, Edney M, Jones B, Rossnagel B, Legge W, Briggs K, Irvine R, Falk D (1997) Regions of the genome that affect grain and malt quality in a North American two-row barley cross. Crop Sci 37:544–554CrossRefGoogle Scholar
  38. Nair S, Ullrich S, Blake T, Cooper B, Griffey C, Hayes P, Hole D, Horsley R, Obert D, Smith K (2010) Variation in kernel hardness and associated traits in US barley breeding lines. Cereal Chem 87:461–466CrossRefGoogle Scholar
  39. Panozzo J, Eckermann P, Mather D, Moody D, Black C, Collins H, Barr A, Lim P, Cullis BR (2007) QTL analysis of malting quality traits in two barley populations. Crop Pasture Sci 58:858–866CrossRefGoogle Scholar
  40. Poland JA, Brown PJ, Sorrells ME, Jannink J-L (2012) Development of high-density genetic maps for barley and wheat using a novel two-enzyme genotyping-by-sequencing approach. PLoS One 7:e32253CrossRefPubMedPubMedCentralGoogle Scholar
  41. Psota V, Vejražka K, Faměra O, Hrčka M (2007) Relationship between grain hardness and malting quality of barley (Hordeum vulgare L.). J Inst Brew 113:80–86CrossRefGoogle Scholar
  42. Qi J-C, Chen J-X, Wang J-M, Wu F-B, Cao L-P, Zhang G-P (2005) Protein and hordein fraction content in barley seeds as affected by sowing date and their relations to malting quality. J Zhejiang Univ Sci B 6:1069CrossRefPubMedPubMedCentralGoogle Scholar
  43. Rae S, Macaulay M, Ramsay L, Leigh F, Matthews D, O’Sullivan D, Donini P, Morris P, Powell W, Marshall D, Waugh R, Thomas W (2007) Molecular barley breeding. Euphytica 158:295–303CrossRefGoogle Scholar
  44. Roumeliotis S, Tansing P (2004) SA barley improvement program barley quality report 2001 & 2002 seasons. The University of Adelaide, AdelaideGoogle Scholar
  45. Schmalenbach I, Pillen K (2009) Detection and verification of malting quality QTLs using wild barley introgression lines. Theor Appl Genet 118:1411–1427CrossRefPubMedPubMedCentralGoogle Scholar
  46. Slakeski N, Fincher GB (1992) Developmental regulation of (1 → 3, 1 → 4)-β-glucanase gene expression in barley tissue-specific expression of individual isoenzymes. Plant Physiol 99:1226–1231CrossRefPubMedPubMedCentralGoogle Scholar
  47. Swanston J (1995) Effects on barley grain size, texture and modification during malting associated with three genes on chromosome 1. J Cereal Sci 22:157–161CrossRefGoogle Scholar
  48. Thomas W (2003) Prospects for molecular breeding of barley. Ann Appl Biol 142:1–12CrossRefGoogle Scholar
  49. Turuspekov Y, Beecher B, Darlington Y, Bowman J, Blake T, Giroux M (2008) Locus sequence variation and endosperm texture in spring barley. Crop Sci 48:1007–1019CrossRefGoogle Scholar
  50. Vejražka K (2014) The evaluation of barley grain hardness measured by Do-Corder, as a malting quality parameter. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 55:197–204CrossRefGoogle Scholar
  51. von Korff M, Wang H, Léon J, Pillen K (2008) AB-QTL analysis in spring barley: III. Identification of exotic alleles for the improvement of malting quality in spring barley (H. vulgare ssp. spontaneum). Mol Breed 21:81–93CrossRefGoogle Scholar
  52. Walker CK, Panozzo J, Ford R, Eckermann P, Moody D, Lehmensiek A, Appels R (2011) Chromosomal loci associated with endosperm hardness in a malting barley cross. Theor Appl Genet 122:151–162CrossRefPubMedGoogle Scholar
  53. Walker CK, Ford R, Muñoz-Amatriaín M, Panozzo JF (2013) The detection of QTLs in barley associated with endosperm hardness, grain density, grain size and malting quality using rapid phenotyping tools. Theor Appl Genet 126:2533–2551CrossRefPubMedGoogle Scholar
  54. Wenzl P, Li H, Carling J, Zhou M, Raman H, Paul E, Hearnden P, Maier C, Xia L, Caig V (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits. BMC Genomics 7:206CrossRefPubMedPubMedCentralGoogle Scholar
  55. Yan L, Fu D, Li C, Blechl A, Tranquilli G, Bonafede M, Sanchez A, Valarik M, Yasuda S, Dubcovsky J (2006) The wheat and barley vernalization gene VRN3 is an orthologue of FT. Proc Natl Acad Sci 103:19581–19586CrossRefPubMedPubMedCentralGoogle Scholar
  56. Zale J, Clancy J, Ullrich S, Jones B, Hayes P (2000) Summary of barley malting quality QTLs mapped in various populations. Barley Genet Newsl 30:44–54Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Suong T. Cu
    • 1
  • Timothy J. March
    • 2
  • Susan Stewart
    • 2
  • Sophia Degner
    • 2
  • Stewart Coventry
    • 2
  • Amanda Box
    • 2
  • Doug Stewart
    • 3
  • Birgitte Skadhauge
    • 4
  • Rachel A. Burton
    • 1
  • Geoffrey B. Fincher
    • 1
  • Jason Eglinton
    • 2
  1. 1.Australian Research Council Centre of Excellence in Plant Cell WallsThe University of AdelaideGlen OsmondAustralia
  2. 2.School of Agriculture, Food and WineThe University of AdelaideGlen OsmondAustralia
  3. 3.Cargill Malt, CargillStepneyAustralia
  4. 4.Carlsberg Research LaboratoryCopenhagen VDenmark

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