Molecular Breeding

, 36:51

Genotyping-by-sequencing to remap QTL for type II Fusarium head blight and leaf rust resistance in a wheat–tall wheatgrass introgression recombinant inbred population

  • Xiangye Xiao
  • Herbert W. Ohm
  • Greg J. Hunt
  • Jesse A. Poland
  • Lingrang Kong
  • Jill A. Nemacheck
  • Christie E. Williams
Article

DOI: 10.1007/s11032-016-0472-0

Cite this article as:
Xiao, X., Ohm, H.W., Hunt, G.J. et al. Mol Breeding (2016) 36: 51. doi:10.1007/s11032-016-0472-0
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Abstract

Fusarium graminearum Schwabe (Fusarium head blight, FHB) and Puccinia triticina Eriks (leaf rust) are two major fungal pathogens posing a continuous threat to the wheat crop; consequently, identifying resistance genes from various sources is always of importance to wheat breeders. We identified tightly linked single nucleotide polymorphism (SNP) markers for the FHB resistance quantitative trait locus (QTL) Qfhs.pur-7EL and the leaf rust resistance locus Lr19 using genotyping-by-sequencing (GBS) in a wheat–tall wheatgrass introgression-derived recombinant inbred line (RIL) population. One thousand and seven hundred high-confidence SNPs were used to conduct the linkage and QTL analysis. Qfhs.pur-7EL was mapped to a 2.9 cM region containing four markers within a 43.6 cM segment of wheatgrass chromosome 7el2 that was translocated onto wheat chromosome 7DL. Lr19 from 7el1 was mapped to a 1.21 cM region containing two markers in the same area, in repulsion. Five lines were identified with the resistance-associated SNP alleles for Qfhs.pur-7EL and Lr19 in coupling. Two SNP markers in the Qfhs.pur-7EL region were converted into PCR-based KASP markers. Investigation of the genetic characteristics of the parental lines of this RIL population indicated that they are translocation lines in two different wheat cultivar genetic backgrounds instead of 7E–7D substitution lines in Thatcher wheat background, as previously reported in the literature.

Keywords

Fusarium head blight resistance Leaf rust resistance Wheat Tall wheatgrass Substitution lines KASP assay 

Supplementary material

11032_2016_472_MOESM1_ESM.docx (23 kb)
Supplementary material 1 (DOCX 22 kb)
11032_2016_472_MOESM2_ESM.docx (38 kb)
Supplementary material 2 (DOCX 37 kb)
11032_2016_472_MOESM3_ESM.docx (58 kb)
Supplementary material 3 (DOCX 58 kb)
11032_2016_472_MOESM4_ESM.docx (30 kb)
Supplementary material 4 (DOCX 29 kb)

Copyright information

© Springer Science+Business Media Dordrecht (outside the USA) 2016

Authors and Affiliations

  • Xiangye Xiao
    • 1
  • Herbert W. Ohm
    • 1
  • Greg J. Hunt
    • 2
  • Jesse A. Poland
    • 3
  • Lingrang Kong
    • 4
  • Jill A. Nemacheck
    • 5
  • Christie E. Williams
    • 1
    • 5
  1. 1.Department of AgronomyPurdue UniversityWest LafayetteUSA
  2. 2.Department of EntomologyPurdue UniversityWest LafayetteUSA
  3. 3.Department of Plant PathologyKansas State UniversityManhattanUSA
  4. 4.State Key Laboratory of Crop BiologyShandong Agricultural UniversityTaianChina
  5. 5.USDA-ARS, Crop Production and Pest Control Research UnitWest LafayetteUSA

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