Molecular Breeding

, 35:28 | Cite as

Development of user-friendly markers for the pvr1 and Bs3 disease resistance genes in pepper

  • William L. Holdsworth
  • Michael MazourekEmail author
Short Communication


Viruses and Xanthomonas spp., the causal agent of bacterial spot, are serious threats to pepper (Capsicum spp.) production in the USA. For decades, pepper growers have relied on host plant resistance as a first line of defense against these pathogens, and pepper breeders have deployed, cloned, and characterized a growing number of resistance genes. Molecular markers within or linked to these genes have facilitated rapid screening of breeding populations for resistance alleles relative to methods requiring pathogen inoculation. We have developed user-friendly markers in coding regions for the cloned pvr1 and Bs3 resistance genes using the Kompetitive Allele-Specific PCR (KASP) genotyping system in order to increase the robustness and throughput by which these loci are screened. The KASP markers are inexpensive, fast to process, and easily scored.


KASP Marker-assisted selection pepper Bs3 pvr1 Disease resistance Capsicum 



We thank Bob Stall from the University of Florida for contributing seed of ‘ECW,’ ‘ECW10R,’ ‘ECW20R,’ ‘ECW30R,’ and ‘ECW123R’ and James Frantz and Molly Jahn for contributing seeds of the CC population. We thank John Murphy for supplying the PVY-NN inoculum and Samantha Klasfeld for laboratory assistance. We thank Mary Kreitinger and Lindsay Wyatt for thoughtful edits of this manuscript. Support for William Holdsworth was provided by USDA National Institute of Food and Agriculture Plant Breeding and Education Grant No. 2010-85117-20551.


  1. Blauth JR (1994) Genetic analysis of resistance to pepper mottle potyvirus and tobacco etch potyvirus in pepper: genus Capsicum. Dissertation, Cornell UniversityGoogle Scholar
  2. Cook AA, Guevara YG (1984) Hypersensitivity in Capsicum chacoense to race 1 of the bacterial spot pathogen of pepper. Plant Dis 68(4):329–330CrossRefGoogle Scholar
  3. Cook AA, Stall RE (1963) Inheritance of resistance in pepper to bacterial spot. Phytopathol 53:1060–1062Google Scholar
  4. Doyle JJ, Doyle JL (1987) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
  5. European and Mediterranean Plant Protection Organization (2013) Xanthomonas spp. (Xanthomonas euvesicatoria, Xanthomonas gardneri, Xanthomonas perforans, Xanthomonas vesicatoria) causing bacterial spot of tomato and sweet pepper. OEPP/EPPO Bull 43(1):7–20CrossRefGoogle Scholar
  6. Gianessi LP, Reigner N (2005) The value of fungicides in U.S. crop production. CropLife Foundation, WashingtonGoogle Scholar
  7. Greenleaf WH (1956) Inheritance of resistance to tobacco-etch virus in Capsicum frutescens and in Capsicum annuum. Phytopathol 46:371–375Google Scholar
  8. Horsfall JG, McDonnell AD (1940) Variety susceptibility of peppers to bacterial spot. Plant Dis Report 24:34–36Google Scholar
  9. Kang B-C, Yeam I, Frantz JD, Murphy JF, Jahn MM (2005) The pvr1 locus in Capsicum encodes a translation initiation factor eIF4E that interacts with Tobacco etch virus VPg. Plant J 42(3):392–405CrossRefPubMedGoogle Scholar
  10. Kim BS, Hartmann RW (1985) Inheritance of a gene (Bs3) conferring hypersensitive resistance to Xanthomonas campestris pv. vesicatoria in pepper (Capsicum annuum). Plant Dis 69(3):233–235Google Scholar
  11. Kousik CS, Ritchie DF (1998) Response of bell pepper cultivars to bacterial spot pathogen races that individually overcome major resistance genes. Plant Dis 82(2):181–186CrossRefGoogle Scholar
  12. Kyle MM, Palloix A (1997) Proposed revision of nomenclature for potyvirus resistance genes in Capsicum. Euphytica 97:183–188CrossRefGoogle Scholar
  13. Mazourek M, Wyatt LE (2013) Candidate gene approaches in Capsicum. In: Kang B-C, Kole C (eds) Genetics, genomics and breeding of peppers and eggplants. CRC Press, London, pp 56–76CrossRefGoogle Scholar
  14. Murphy JF, Blauth JR, Livingstone KD, Lackney VK, Jahn MK (1998) Genetic mapping of the pvr1 locus in Capsicum spp. and evidence that distinct potyvirus resistance loci control responses that differ at the whole plant and cellular levels. Mol Plant Microbe Interact 11(10):943–951CrossRefGoogle Scholar
  15. Pernezny K, Kucharek T (2011) Some common diseases of pepper in Florida. University of Florida.
  16. Pernezny KL, Roberts PD, Murphy JF, Goldberg NP (2003) Compendium of pepper diseases. The American Phytopathological Society, St. PaulGoogle Scholar
  17. Römer P, Hahn S, Jordan T, Strauss T, Bonas U, Lahaye T (2007) Plant pathogen recognition mediated by promoter activation of the pepper Bs3 resistance gene. Science 318(5850):645–648CrossRefPubMedGoogle Scholar
  18. Römer P, Jordan T, Lahaye T (2010) Identification and application of a DNA-based marker that is diagnostic for the pepper (Capsicum annuum) bacterial spot resistance gene Bs3. Plant Breed 129:737–740CrossRefGoogle Scholar
  19. Ruffel S, Dussault M-H, Palloix A, Moury B, Bendahmane A, Robaglia C, Caranta C (2002) A natural recessive resistance gene against potato virus Y in pepper corresponds to the eukaryotic initiation factor 4E (eIF4E). Plant J 32(6):1067–1075CrossRefPubMedGoogle Scholar
  20. Sahin F, Miller SA (1998) Resistance in Capsicum pubescens to Xanthomonas campestris pv. vesicatoria pepper race 6. Plant Dis 82(7):794–799CrossRefGoogle Scholar
  21. Smith R, Aguiar JL, Baameur A, Cahn M, Cantwell M, de la Fuente M, Hartz T, Koike S, Molinar R, Natwick E, Suslow T, Takele E (2011) Chile pepper production in California. University of California Agriculture and Natural Resources Communication ServicesGoogle Scholar
  22. Stall RE, Jones JB, Minsavage GV (2009) Durability of resistance in tomato and pepper to Xanthomonads causing bacterial spot. Annu Rev Phytopathol 47:265–284CrossRefPubMedGoogle Scholar
  23. USDA (2013) Vegetables: 2012 SummaryGoogle Scholar
  24. Yeam I, Kang B-C, Lindeman W, Frantz JD, Faber N, Jahn MM (2005) Allele-specific CAPS markers based on point mutations in resistance alleles at the pvr1 locus encoding eIF4E in Capsicum. Theor Appl Genet 112:178–186CrossRefPubMedGoogle Scholar
  25. Zitter TA (1972) Naturally occurring pepper virus strains in south Florida. Plant Dis Report 56:586–590Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Section of Plant Breeding and GeneticsCornell UniversityIthacaUSA

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