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Molecular Breeding

, 35:52 | Cite as

Characterization of resistance genes to rice blast fungus Magnaporthe oryzae in a “Green Revolution” rice variety

  • Yan Liu
  • Xinshuai Qi
  • Nelson D. Young
  • Kenneth M. Olsen
  • Ana L. Caicedo
  • Yulin Jia
Article

Abstract

The indica rice variety Dee Geo Woo Gen (DGWG) was the source of the semidwarf gene (SD1) which played an important role in the Green Revolution. In the present study, resistance (R) genes to the US race (isolate) IB54 of Magnaporthe oryzae, causal agent of rice blast disease, was investigated. Two recombinant inbred line mapping populations, consisting of 175 and 224 individuals derived from crosses of DGWG with the straw hull weedy rice type PI653435 (AR-2001-1135; S population) and the black hull type PI653419 (MS-1996-9; B population), respectively, were used for mapping blast R genes and quantitative trait loci (QTLs). Two high-resolution linkage maps with 6,513 (S population) and 14,382 (B population) single nucleotide polymorphic markers derived from genotyping-by-sequencing data were used to map R genes. Two partial resistance QTLs, qBR1.1 and qBR6.1, and one major resistance QTL, qBR11.1, were identified in the B population. One partial resistance QTL, qBR6.1, and one major resistance QTL, qBR11.1, were confirmed with the S population. The total phenotypic variation of three resistance QTLs was 51 %, ranging from 1.12 to 47.62 %, in the B population. All three resistance QTLs were localized to relatively small genomic regions. The major resistance QTL, qBR11.1, was mapped to a 129-kb region on chromosome 11 near nine known blast R genes. Within this 129-kb region, three genes encoding putative nucleotide-binding site and leucine-rich repeat (LRR) disease resistance proteins and three genes encoding WRKY transcription factors WRKY61, WRKY63, and WRKY41 were identified as candidate genes of qBR11.1 and tentatively designated as Pi66(t). Identification of blast R genes in DGWG should help continued deployment of useful genes for improving crop productivity and resistance to rice blast disease.

Keywords

DGWG Rice blast resistance R gene GBS Green Revolution Pi66(t) 

Notes

Acknowledgments

We thank Marcos André Nohatto, Michael Lin, and Tracy Bianco for their excellent technical support. This project was funded in part by the National Science Foundation Plant Genome Research Program (NSF award IOS-1032023). The US Department of Agriculture is an equal opportunity provider and employer.

Supplementary material

11032_2015_256_MOESM1_ESM.tif (95 kb)
Supplementary Fig. 1 Logarithm of odds (LOD) curves of R QTLs identified using B and S population. a LOD curves of three resistance (R) QTLs identified using B population. The significant threshold of LOD is 3.65 as indicated by the red dotted line. The LOD peak value is 8.58 for qBR1.1 on chromosome 1, 7.02 for qBR6.1 on chromosome 6, and 57.5 for qBR11.1 on chromosome 11. b LOD curves of two R QTLs identified using S population. The significant threshold of LOD is 3.67 as indicated by the red dotted line. The LOD peak value is 8.72 for qBR6.1 on chromosome 6 and 24.8 for qBR11.1 on chromosome 11 (TIFF 95 kb)
11032_2015_256_MOESM2_ESM.tif (215 kb)
Supplementary Fig. 2 Disease reactions of three parental lines, DGWG, RR20, and RR9, to blast race IB54. DGWG is resistant to IB54, while RR20 is susceptible and RR9 is partially resistant (TIFF 215 kb)

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Copyright information

© Springer Science+Business Media Dordrecht (outside the USA) 2015

Authors and Affiliations

  • Yan Liu
    • 1
    • 2
  • Xinshuai Qi
    • 3
  • Nelson D. Young
    • 4
  • Kenneth M. Olsen
    • 3
  • Ana L. Caicedo
    • 4
  • Yulin Jia
    • 2
  1. 1.Rice Research and Extension CenterUniversity of ArkansasStuttgartUSA
  2. 2.Dale Bumpers National Rice Research CenterUSDA Agricultural Research ServiceStuttgartUSA
  3. 3.Department of BiologyWashington University in St. LouisSt. LouisUSA
  4. 4.Biology DepartmentUniversity of MassachusettsAmherstUSA

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