Flanking SNP markers for vicine–convicine concentration in faba bean (Vicia faba L.)
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The pyrimidine glycosides, vicine and convicine, limit the use of faba bean (Vicia faba L.) as food and feed. A single recessive gene, vc-, is responsible for a lowered vicine–convicine concentration. The biosynthetic pathway of these closely related compounds is not known, and the nearest available markers are several cM away from vc-. Improved markers would assist breeding and help to identify candidate genes. A segregating population of 210 F5 recombinant inbred lines was developed from the cross of Mélodie/2 (low vicine–convicine) × ILB 938/2 (normal vicine–convicine), and vicine–convicine concentrations were determined twice on each line. The population was genotyped with a set of 188 SNPs. A strong, single QTL for vicine–convicine concentration was identified on chromosome I, flanked by markers 1.0 cM away on one side and 2.6 cM on the other. The interval defined by these markers in the model species Medicago truncatula includes about 340 genes, but no candidate genes were identified. Further fine mapping should lead to the identification of tightly linked markers as well as narrowing down the search for candidate regulatory or biosynthetic genes which could underlie the vc- locus.
KeywordsVicine and convicine Faba bean SNPs QTL Medicago truncatula
H. Khazaei thanks the Emil Aaltonen Foundation (Emil Aaltosen Säätiö) and Niemi-säätiö for their financial support. The project was further supported by the University of Helsinki and the Niemi-Säätiö. We thank Markku Tykkyläinen, Sanna Peltola, and Sini Lindström for their assistance in the glasshouse work at the University of Helsinki. Kirsi Peltoniemi (Boreal Plant Breeding Ltd) is thanked for providing the spectrophotometric vicine–convicine results. Prof. Wolfgang Link (Georg-August-University, Göttingen, Germany) kindly provided the seeds of the parental lines.
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