Fine-mapping of the apple scab resistance locus Rvi12 (Vb) derived from ‘Hansen’s baccata #2’
- 433 Downloads
Apple scab is a disease caused by the fungus Venturia inaequalis, which leads to significant economic losses in apple production especially in temperate regions. Breeding programmes are attempting to introgress scab resistance genes from wild apple into commercial cultivars to control the disease. Most of the commercially available scab-resistant varieties to date rely on the Rvi6 (Vf) resistance gene from Malus floribunda 821. The evolution of new pathotypes of V. inaequalis, which have caused the breakdown of Rvi6-based resistance, at least in northern Europe, highlights the need for the characterisation and pyramiding of scab resistance genes from different sources for durable disease resistance. In this study, the scab resistance gene Rvi12 from Malus baccata ‘Hansen’s baccata #2’ was confirmed as mapping to apple linkage group 12 in the cross ‘Gala’ × ‘Hansen’s baccata #2’ in an interval between SSR markers Hi02d05 and CH02h11b. Using the ‘Golden Delicious’ genome sequence, novel SSR markers and SNPs were identified in the Rvi12 mapping interval and mapped in an extended mapping population of 1,285 plants. Rvi12 was fine-mapped to an interval spanning 958 kb of the ‘Golden Delicious’ genome sequence. The 18 SNPs fine-mapped to the Rvi12 interval were screened in eight apple breeding founders, and for 16 of the 18 SNPs, the alleles linked in coupling with the Rvi12 resistance locus were found only in ‘Hansen’s baccata #2’. The SNPs identified will thus be useful for the efficient identification of apple genotypes carrying the Rvi12 resistance locus.
KeywordsSNP SSR Marker-assisted breeding Rosaceae Pome fruits Genomics
The work was funded in part by grants from the Autonomous Province of Trento, the Agroalimentare research AGER project—Apple fruit quality in the post-genomic era, from breeding new genotypes to post-harvest: nutrition and health (Grant No. 2010-2119) and the EU seventh Framework Programme by the FruitBreedomics Project Number 255582: Integrated approach for increasing breeding efficiency in fruit tree crops. The views expressed are the sole responsibility of the authors and do not necessarily reflect the views of the European Commission.
- Crandall C (1926) Apple breeding at the University of Illinois. Illinois Agric Exp Stn Bull 275:341–600Google Scholar
- Dayton D, Williams E (1968) Independent genes in Malus for resistance to Venturia inaequalis. Proc Am Soc Hortic Sci 92:89–93Google Scholar
- Dayton D, Mowry J, Hough L, Bailey C, Williams E, Janick J, Emerson F (1970) ‘Prima’, an early fall apple with resistance to scab. Fruit Var Hortic Dig 24:20–22Google Scholar
- Hemmat M, Brown S, Aldwinckle H, Mehlenbacher S, Weeden N, Janick J (2003) Identification and mapping of markers for resistance to apple scab from ‘Antonovka’ and ‘Hansen’s baccata #2’. Acta Hortic 622:153–161Google Scholar
- Hough L, Shay J, Dayton D (1953) Apple scab resistance from Malus floribunda Sieb. Proc Am Soc Hortic Sci 62:341–347Google Scholar
- MacHardy W (1996) Apple Scab Biology, Epidemiology, and Management. APS Press, St. PaulGoogle Scholar
- Parisi L, Fouillet V, Schouten HJ, Groenwold R, Laurens F, Didelot F, Evans K, Fischer C, Gennari F, Kemp H, Lateur M, Patocchi A, Thissen J, Tsipouridis C (2004) Variability of the pathogenicity of Venturia inaequalis in Europe. Acta Hortic 663:107–113Google Scholar
- Silfverberg-Dilworth E (2004) Identification of HcrV2 as an apple scab resistance gene and characterisation of HcrVf control sequences. Dissertation, ETHGoogle Scholar