Molecular Breeding

, Volume 34, Issue 4, pp 2033–2048 | Cite as

Construction of a genetic linkage map of an interspecific diploid blueberry population and identification of QTL for chilling requirement and cold hardiness

  • Lisa J. Rowland
  • Elizabeth L. Ogden
  • Nahla Bassil
  • Emily J. Buck
  • Susan McCallum
  • Julie Graham
  • Allan Brown
  • Claudia Wiedow
  • A. Malcolm Campbell
  • Kathleen G. Haynes
  • Bryan T. Vinyard


A genetic linkage map has been constructed from an interspecific diploid blueberry population [(Vaccinium darrowii Fla4B × Vaccinium corymbosum W85-20) F1#10 × V. corymbosum W85-23] designed to segregate for cold hardiness and chilling requirement. The map is comprised of 12 linkage groups (equivalent to the haploid chromosome number of diploid blueberry) and totals 1,740 cM. Included on the map are 265 markers based on simple sequence repeats, expressed sequence tag-polymerase chain reactions, single nucleotide polymorphisms, and randomly amplified polymorphic DNAs. The estimated map coverage is 89.9 %, and the average distance between markers is 7.2 cM. The mapping population was evaluated for 2 years (2009 and 2010) for mid-winter bud cold hardiness and for 3 years (2011–2013) for chilling requirement under controlled conditions. Broad-sense heritability of both cold hardiness and chilling requirement was quite high under these conditions with values of 0.88 and 0.86, respectively. One quantitative trait locus/loci (QTL) for cold hardiness and one for chilling requirement were identified that were consistent over at least 2 years. A second weaker QTL for chilling requirement was detected in only one of the 3 years.


EST-PCR SNP SSR Markers Vaccinium corymbosum Vaccinium darrowii 



Cold hardiness


Chilling requirement


Chill units resulting in 50 % floral bud break


Expressed sequence tag-polymerase chain reaction


Broad-sense heritability


High-resolution melting


Interval mapping


Logarithm of the odds


Lethal temperature causing 50 % injury


Permutation test


Quantitative trait locus/loci


Randomly amplified polymorphic DNA


Restricted multiple QTL mapping


Single nucleotide polymorphism


Sequence read archive


Simple sequence repeat



The authors gratefully acknowledge all the high school and college students who participated in this project as part of an internship or other training opportunity. We specifically would like to acknowledge the students from the University of Maryland (Brianna Driscoll, Dana Robinson, and Jenny Lindvall), who worked in Dr. Rowland’s lab on the mapping project, and the students from Davidson College (Mark Angel, Erich Baker, Spencer Chadinha, Stewart Dalton, Aaron Deal, Catherine Doyle, Tim Keating, David Lloyd, Austin Mudd, Mike Nuttle, Shamita Punjabi, and Daniel Tuerff), who worked in Dr. Campbell’s class to design SSRs near genes of interest. We would also like to thank Barbara Gilmore, April Nyberg, Elisabeth Alperin, and Jeremy Jones, who were involved in screening the mapping parents for SSR polymorphism. This project was partially funded by USDA-ARS Project 1245-21000-185-00D and USDA-CSREES Specialty Crop Research Initiative Grant 2008-51180-04861 entitled ‘Generating Genomic Tools for Blueberry Improvement.’ Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture or any of the other agencies involved in this research.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11032_2014_161_MOESM1_ESM.xlsx (41 kb)
Supplementary material 1 (XLSX 40 kb)
11032_2014_161_MOESM2_ESM.docx (12 kb)
Supplementary material 2 (DOCX 11 kb)


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Copyright information

© Springer Science+Business Media Dordrecht (outside the USA) 2014

Authors and Affiliations

  • Lisa J. Rowland
    • 1
  • Elizabeth L. Ogden
    • 1
  • Nahla Bassil
    • 2
  • Emily J. Buck
    • 3
  • Susan McCallum
    • 4
  • Julie Graham
    • 4
  • Allan Brown
    • 5
  • Claudia Wiedow
    • 3
  • A. Malcolm Campbell
    • 6
  • Kathleen G. Haynes
    • 1
  • Bryan T. Vinyard
    • 7
  1. 1.Genetic Improvement of Fruits and Vegetables Laboratory, Henry A. Wallace Beltsville Agricultural Research Center-WestUnited States Department of Agriculture, Agricultural Research ServiceBeltsvilleUSA
  2. 2.National Clonal Germplasm RepositoryUnited States Department of Agriculture, Agricultural Research ServiceCorvallisUSA
  3. 3.The New Zealand Institute for Plant and Food Research LtdPalmerston NorthNew Zealand
  4. 4.Department of GeneticsJames Hutton InstituteDundeeScotland, UK
  5. 5.Department of Horticultural Science, Plants for Human Health InstituteNorth Carolina State UniversityKannapolisUSA
  6. 6.Department of BiologyDavidson CollegeDavidsonUSA
  7. 7.Henry A. Wallace Beltsville Agricultural Research Center-West, Biometrical Consulting ServiceUnited States Department of Agriculture, Agricultural Research ServiceBeltsvilleUSA

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