Molecular Breeding

, Volume 34, Issue 2, pp 691–700 | Cite as

Rice blast resistance gene Pikahei-1(t), a member of a resistance gene cluster on chromosome 4, encodes a nucleotide-binding site and leucine-rich repeat protein

  • Xin Xu
  • Nagao Hayashi
  • Chun-Tai Wang
  • Shuichi Fukuoka
  • Shinji Kawasaki
  • Hiroshi Takatsuji
  • Chang-Jie Jiang
Article

Abstract

Pikahei-1(t) is the strongest quantitative trait locus (QTL) for blast resistance in upland rice cv. Kahei, which has strong field resistance to the rice blast disease. A high-quality bacterial artificial chromosome library was used to fine-map Pikahei-1(t) within ~300 kb on the 31-Mb region on rice chromosome 4. Of the 42 predicted open reading frames, seven resistance gene analogs (RGAs) with the nucleotide-binding site and leucine-rich repeat (NBS-LRR) domain were identified. Among these, RGA1, 2, 3, 5, and 7, but not RGA4 and 6, were found to be expressed in Kahei and monogenic lines containing Pikahei-1(t). Blast inoculation of transgenic rice lines carrying the genomic fragment of each RGA revealed that only RGA3 was associated with blast resistance. On the basis of these results, we concluded that RGA3 is the Pikahei-1(t) and named it Pi63. Pi63 encoded a typical coiled-coil-NBS-LRR protein and showed isolate-specificity. These results suggest that Pi63 behaves like a typical Resistance (R) gene, and the strong and broad-spectrum resistance of Kahei is dependent on natural pyramiding of multiple QTLs. The blast resistance levels of Pi63 were closely correlated with its gene expression levels, indicating a dose-dependent response of Pi63 function in rice resistance. Pi63 is the first cloned R gene in the R gene cluster on rice chromosome 4, and its cloning might facilitate genomic dissection of this cluster region.

Keywords

Rice Blast resistance Field resistance Pikahei-1(t) Pi63 NBS-LRR 

Notes

Acknowledgments

We thank Dr. T. Manabe (Ibaraki Agricultural Center, Plant-Biotechnology Institute) for providing the Hitachi IL-1 rice line and Drs. H. Yamane, J.Z. Wu, and T. Matsumoto (National Institute of Agrobiological Sciences, Japan) for constructing the BAC library of cv. Kahei. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics-based Technology for Agricultural Improvement, LCT-0013).

Supplementary material

11032_2014_67_MOESM1_ESM.docx (37 kb)
Supplementary material 1 (DOCX 37 kb)
11032_2014_67_MOESM2_ESM.pdf (165 kb)
Supplementary material 2 (PDF 165 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Xin Xu
    • 1
  • Nagao Hayashi
    • 2
  • Chun-Tai Wang
    • 1
  • Shuichi Fukuoka
    • 2
  • Shinji Kawasaki
    • 2
  • Hiroshi Takatsuji
    • 2
  • Chang-Jie Jiang
    • 2
  1. 1.Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, Key Laboratory for Biotechnology of National Commission for Nationalities, College of Life ScienceSouth-Central University for NationalitiesWuhanPeople’s Republic of China
  2. 2.National Institute of Agrobiological SciencesTsukubaJapan

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