Rice blast resistance gene Pikahei-1(t), a member of a resistance gene cluster on chromosome 4, encodes a nucleotide-binding site and leucine-rich repeat protein
- 982 Downloads
Pikahei-1(t) is the strongest quantitative trait locus (QTL) for blast resistance in upland rice cv. Kahei, which has strong field resistance to the rice blast disease. A high-quality bacterial artificial chromosome library was used to fine-map Pikahei-1(t) within ~300 kb on the 31-Mb region on rice chromosome 4. Of the 42 predicted open reading frames, seven resistance gene analogs (RGAs) with the nucleotide-binding site and leucine-rich repeat (NBS-LRR) domain were identified. Among these, RGA1, 2, 3, 5, and 7, but not RGA4 and 6, were found to be expressed in Kahei and monogenic lines containing Pikahei-1(t). Blast inoculation of transgenic rice lines carrying the genomic fragment of each RGA revealed that only RGA3 was associated with blast resistance. On the basis of these results, we concluded that RGA3 is the Pikahei-1(t) and named it Pi63. Pi63 encoded a typical coiled-coil-NBS-LRR protein and showed isolate-specificity. These results suggest that Pi63 behaves like a typical Resistance (R) gene, and the strong and broad-spectrum resistance of Kahei is dependent on natural pyramiding of multiple QTLs. The blast resistance levels of Pi63 were closely correlated with its gene expression levels, indicating a dose-dependent response of Pi63 function in rice resistance. Pi63 is the first cloned R gene in the R gene cluster on rice chromosome 4, and its cloning might facilitate genomic dissection of this cluster region.
KeywordsRice Blast resistance Field resistance Pikahei-1(t) Pi63 NBS-LRR
We thank Dr. T. Manabe (Ibaraki Agricultural Center, Plant-Biotechnology Institute) for providing the Hitachi IL-1 rice line and Drs. H. Yamane, J.Z. Wu, and T. Matsumoto (National Institute of Agrobiological Sciences, Japan) for constructing the BAC library of cv. Kahei. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics-based Technology for Agricultural Improvement, LCT-0013).
- Ahn SN, Kim DM, Ju HG, Kang JW, Han SS (2013) A new rice variety ‘Hwaweon 5’ with durable resistance to rice blast. Korean J Breed Sci 45:142–147Google Scholar
- Cesari S, Thilliez G, Ribot C, Chalvon V, Michel C, Jauneau A, Rivas S, Alaux L, Kanzaki H, Okuyama Y, Morel JB, Fournier E, Tharreau D, Terauchi R, Kroj T (2013) The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe oryzae effectors AVR-Pia and AVR1-CO39 by direct binding. Plant Cell 25:1463–1481PubMedCentralPubMedCrossRefGoogle Scholar
- Endo T, Yamaguchi M, Kaji R, Nakagomi K, Kataoka T, Yokogami N, Nakamura T, Ishikawa G, Yonemaru J, Nishio T (2012) Close linkage of a blast resistance gene, Pias(t), with a bacterial leaf blight resistance gene, Xa1-as(t), in a rice cultivar ‘Asominori’. Breed Sci 62:334–339PubMedCentralPubMedCrossRefGoogle Scholar
- Hayashi N, Inoue H, Kato T, Funao T, Shirota M, Shimizu T, Kanamori H, Yamane H, Hayano-Saito Y, Matsumoto T, Yano M, Takatsuji H (2010) Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication. Plant J 64:498–510PubMedCrossRefGoogle Scholar
- Koizumi S, Fuji S (1995) Variation of field resistance to leaf blast in a rice isolate, Chubu 32, due to isolates of the pathogen. Res Bull Aichi Agric Res 27:85–93 (in Japanese with English summery)Google Scholar
- Lee SK, Song MY, Seo YS, Kim HK, Ko S, Cao PJ, Suh JP, Yi G, Roh JH, Lee S, An G, Hahn TR, Wang GL, Ronald P, Jeon JS (2009) Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes. Genetics 181:1627–1638PubMedCentralPubMedCrossRefGoogle Scholar
- Wang Y, Wang D, Deng X, Liu J, Sun P, Liu Y, Huang H, Jiang N, Kang H, Ning Y, Wang Z, Xiao Y, Liu X, Liu E, Dai L, Wang GL (2012) Molecular mapping of the blast resistance genes Pi2-1 and Pi51(t) in the durably resistant rice ‘Tianjingyeshengdao’. Phytopathology 102:779–786PubMedCrossRefGoogle Scholar
- Yamaguchi T (1980) General characterizations of blast and blast fungus IV. General experimental methods of blast fungus. Blast disease and breeding for blast resistant varieties of rice. Y. Yamazaki and T. Kozaka. Tokyo, Hakuyusha: 140–174Google Scholar
- Zhao K, Tung CW, Eizenga GC, Wright MH, Ali ML, Price AH, Norton GJ, Islam MR, Reynolds A, Mezey J, McClung AM, Bustamante CD, McCouch SR (2011) Genome-wide association mapping reveals a rich genetic architecture of complex traits in Oryza sativa. Nat Commun 2:467PubMedCentralPubMedCrossRefGoogle Scholar