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Molecular Breeding

, Volume 33, Issue 4, pp 803–811 | Cite as

Mitotype-specific sequences related to cytoplasmic male sterility in Oryza species

  • Hongwei Xie
  • Jie Wang
  • Mingjuan Qian
  • Nengwu Li
  • Yingguo Zhu
  • Shaoqing Li
Article

Abstract

Plant mitochondrial genomes contain a large number of mitotype-specific sequences (MSS) which establish a mitochondrial genome structure distinct from other mitotypes. In rice, nine mitochondrial genomes have been sequenced, which provides us with the possibility of characterizing the MSS of rice and probing their relationship to cytoplasmic male sterility (CMS) in rice. We therefore analyzed the mitochondrial genomes of CW-CMS, LD-CMS, WA-CMS, N and Nipponbare lines, and found 57 MSS with sizes ranging from 102 to 5,745 bp, and with an aggregate length of 92.4 kb. The MSS account for more than 14.5 % of the rice mitochondrial genome and are a significant contributing factor in the variation of mitochondrial genome sizes. Of the MSS tested, 34 MSS exhibited polymorphism among rice lines, and 14 MSS were further confirmed as being specific to CMS. This includes nine MSS specific to sporophytic CMS, three specific to gametophytic CMS, and two shared by all types of CMS. Interestingly, except for CMS genes orf(H)79 and orf352 which are partly or fully overlapping with some MSS fragments, there are ten more open reading frames of unknown function that were detected in CMS-specific MSS, hinting at their possible roles in plant CMS. These novel findings provide us with potential new molecular tools to direct the breeding of CMS lines in hybrid rice breeding programs.

Keywords

Mitotype-specific sequence Cytoplasmic male sterility Mitochondria Molecular marker Oryza species 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation (31071391) and the 973 Program (2011CB100102) of China.

Supplementary material

11032_2013_9993_MOESM1_ESM.doc (1.1 mb)
Supplementary material 1 (DOC 1168 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.State Key Laboratory for Hybrid Rice, College of Life ScienceWuhan UniversityWuhanChina
  2. 2.Jiangxi Super-rice Research and Development CenterJiangxi Academy of Agricultural SciencesNanchangChina

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