Molecular Breeding

, Volume 33, Issue 2, pp 401–414 | Cite as

Overexpression of the transcription factor HvSNAC1 improves drought tolerance in barley (Hordeum vulgare L.)

  • A. M. Al Abdallat
  • J. Y. Ayad
  • J. M. Abu Elenein
  • Z. Al Ajlouni
  • W. A. Harwood
Article

Abstract

NAC proteins constitute a family of plant-specific transcription factors that are involved in many plant cellular processes including responses to abiotic stress. In this study, a cDNA clone encoding the HvSNAC1 transcription factor was isolated from drought-stressed barley using a bioinformatics approach based on amino acid sequence data of the stress-related SNAC1 protein from rice. Phylogenetic analysis of the deduced amino acid sequence of HvSNAC1 showed that this protein belongs to the stress clade of NAC proteins that include SNAC1 and TaNAC2. Expression analysis indicated that the HvSNAC1 gene is strongly induced by different abiotic stresses including drought. Overexpression of HvSNAC1 in barley under the control of a constitutive promoter produced plants that grew normally under well-watered conditions when compared with wild-type plants. Transgenic barley plants overexpressing HvSNAC1 showed higher drought tolerance at different growth stages when compared with wild-type plants. In addition, the constitutive overexpression of HvSNAC1 resulted in improved water status, photosynthetic activity and reduced water loss rate when compared with wild-type plants under drought conditions. Furthermore, the transgenic plants also showed significantly improved productivity, as reflected by the increase in biological yield over the wild-type plants under severe field drought conditions. In conclusion, the HvSNAC1 gene could be a useful tool for improving barley productivity under field drought conditions without impairment in growth under normal field conditions.

Keywords

Abiotic stress Bioinformatics Drought Hordeum vulgare Stomatal conductance Transcription factor 

Notes

Acknowledgments

We gratefully acknowledge Mrs. Samar Misbeh, Miss Shireen Qasrawi and Mr. Mohammad Sheik Omar for technical assistance. This work was supported in part by a Grant from the Scientific Research Fund, Ministry of Higher Education, in part by a Grant from the Deanship of Scientific Research, University of Jordan and in part by a Grant from the International Foundation of Science/Sweden.

Supplementary material

11032_2013_9958_MOESM1_ESM.docx (1.2 mb)
Supplementary material 1 (DOCX 1224 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • A. M. Al Abdallat
    • 1
    • 2
  • J. Y. Ayad
    • 1
  • J. M. Abu Elenein
    • 1
  • Z. Al Ajlouni
    • 3
  • W. A. Harwood
    • 4
  1. 1.Department of Horticulture and Crop Science, Faculty of AgricultureThe University of JordanAmmanJordan
  2. 2.International Center for Agricultural Research in the Dry Areas (ICARDA)AmmanJordan
  3. 3.Department of Plant Production, Faculty of AgricultureJordan University of Science and TechnologyIrbidJordan
  4. 4.John Innes CentreNorwich Research ParkNorwichUK

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