Molecular Breeding

, Volume 33, Issue 1, pp 75–88 | Cite as

Characterization of the rich haplotypes of Viviparous-1A in Chinese wheats and development of a novel sequence-tagged site marker for pre-harvest sprouting resistance

Article

Abstract

Pre-harvest sprouting (PHS) reduces the quality of wheat (Triticum aestivum L.) and the economic value of the grain. Our previous studies characterized the haplotypes of Viviparous-1B (Vp-1B) and its association with PHS resistance in Chinese wheats. The objective of this study was to analyze the haplotypes of Viviparous-1A (Vp-1A) and Viviparous-1D (Vp-1D) in a collection of 103 widely-grown winter wheats in China, and their associations with PHS resistance. In total, 17 Vp-1A haplotypes were explored on chromosome 3A of bread wheat, and were located in three major regions, the third intron, fifth intron and sixth exon, and designated TaVp-1Aam, TaVp-1Aan, TaVp-1Aao, TaVp-1Abm, TaVp-1Abn, TaVp-1Agm, TaVp-1Ahm, TaVp-1Ahn, TaVp-1Aho, TaVp-1Aim, TaVp-1Ain, TaVp-1Aio, TaVp-1Ajm, TaVp-1Ajn, TaVp-1Akm, TaVp-1Alm and TaVp-1Aln, respectively. However, no allelic variation of Vp-1D was found in this set of germplasm. Based on the haplotypes explored and their average germination index values, a novel co-dominant sequence-tagged site marker of the TaVp-1A gene was developed and designated Vp1A3. In most cases, haplotype TaVp-1Agm was associated with higher resistance to PHS. By combining with our previously exploited Vp1B3 marker, the efficiency of marker-assisted selection for PHS-resistant varieties was improved. Moreover, while the haplotype combination of TaVp-1Aam and TaVp-1Ba was associated with greater PHS susceptibility, the haplotype combinations TaVp-1Agm and TaVp-1Bb, TaVp-1Agm and TaVp-1Ba, TaVp-1Aim and TaVp-1Bb, and TaVp-1Aam and TaVp-1Bb could confer higher PHS resistance and be used as potential parental lines for molecular marker-assisted wheat breeding for PHS resistance.

Keywords

Bread wheat Pre-harvest sprouting TaVp-1A STS marker 

Supplementary material

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References

  1. Bailey PC, McKibbin RS, Lenton JR (1999) Genetic map location for orthologous VP1 genes in wheat and rice. Theor Appl Genet 98:281–284CrossRefGoogle Scholar
  2. Bassam BJ, Caetano-Anolles G, Gresshoff PM (1991) Fast and sensitive sliver staining of DNA in polyacrylamide gels. Ann Biochem 196:80–83CrossRefGoogle Scholar
  3. Carrari F, Perez-Flores L, Lijavetzky D, Enciso S, Sanchez R, Benech-Arnold R, Iusem N (2001) Cloning and expression of a sorghum gene with homology to maize vp1, its potential involvement in pre-harvest sprouting resistance. Plant Mol Biol 45:631–640PubMedCrossRefGoogle Scholar
  4. Carson CB, Hattori T, Rosenkrans L, Vasil V, Peterson PA, McCarty DR (1997) The quiescent/colorless alleles of viviparous1 show that the conserved B3 domain of Vp1 is not essential for ABA-regulated gene expression in the seed. Plant J 12:1231–1240PubMedCrossRefGoogle Scholar
  5. Chang S, Puryear J, Cairney J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11:113–116CrossRefGoogle Scholar
  6. Chang C, Zhang HP, Zhao QX, Feng JM, Si HQ, Lu J, Ma CX (2011) Rich allelic variations of Viviparous-1A and their associations with seed dormancy/pre-harvest sprouting of common wheat. Euphytica 179:343–353CrossRefGoogle Scholar
  7. Devos KM, Dubcovsky J, Dvorak J, Chinoy CN, Gale MD (1995) Structural evolution of wheat chromosome 4A, 5A and 7B and its impact on recombination. Theor Appl Genet 91:282–288PubMedCrossRefGoogle Scholar
  8. Flintham J (2000) Different genetic components control coat-imposed and embryo-imposed dormancy in wheat. Seed Sci Res 10:43–50CrossRefGoogle Scholar
  9. Flintham JE, Adlam R, Bassoi M, Holdsworth M, Gale M (2002) Mapping genes for resistance to sprouting damage in wheat. Euphytica 126:39–45CrossRefGoogle Scholar
  10. Gale MD, Lenton JR (1987) Preharvest sprouting in wheat: a complex genetic and physiological problem affecting breadmaking quality in UK wheat. Aspects Appl Biol 15:115–124Google Scholar
  11. Gale KR, Ma W, Zhang W, Rampling L, Hill AS, Appels R, Morris P, Morrel M (2001) Simple high-throughput DNA markers for genotyping in wheat. In: Eastwood R et al (eds) Proceedings of the 10th Australian wheat breeding assembly, pp 26–31Google Scholar
  12. Giraudat J, Hauge BM, Valon C, Smalle J, Parcy F, Goodman HM (1992) Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell 4:1251–1261PubMedCentralPubMedGoogle Scholar
  13. Groos C, Gay G, Perretant MR, Gervais L, Bernard M, Dedryver F, Charmet G (2002) Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white × red grain bread-wheat cross. Theor Appl Genet 104:39–47PubMedCrossRefGoogle Scholar
  14. Hattori T, Terada T, Hamasuna ST (1994) Sequence and functional analysis of the rice gene homologous to maize Vp1. Plant Mol Biol 24:805–810PubMedCrossRefGoogle Scholar
  15. Hill A, Nantel A, Rock CD, Quatrano RS (1996) A conserve domain of the viviparous-1 gene product enhances the DNA binding activity of the bZIP protein EmBP-1 and other transcription factors. J Biol Chem 271:3366–3374PubMedCrossRefGoogle Scholar
  16. Hoecker U, Vasil IK, McCarty DR (1995) Integrated control of seed maturation and germination programs by activator and repressor functions of Viviparous-1 of maize. Genes Dev 9:2459–2469PubMedCrossRefGoogle Scholar
  17. Humphreys DG, Noll J (2002) Methods for characterization of preharvest sprouting tolerance in a wheat breeding program. Euphytica 126:61–65CrossRefGoogle Scholar
  18. Jones HD, Peters NC, Holdsworth MJ (1997) Genotype and environment interact to control dormancy and differential expression of the VIVIPAROUS 1 homologue in embryos of Avena fatua. Plant J 12:911–920PubMedCrossRefGoogle Scholar
  19. Kato K, Nakamura W, Tabiki T, Miura H (2001) Detection of loci controlling seed dormancy on group 4 chromosome of wheat and comparative mapping with rice and barley genomes. Theor Appl Genet 102:980–985CrossRefGoogle Scholar
  20. King DG (1997) Evolutionary tuning knobs. Endeavor 21:36–40CrossRefGoogle Scholar
  21. Kulwal PL, Singh R, Balyan HS, Gupta PK (2004) Genetic basis of pre-harvest sprouting tolerance using single-locus and two-locus QTL analyses in bread wheat. Funct Integr Genom 4:94–101CrossRefGoogle Scholar
  22. Lenton J (2001) Opportunities for the manipulation of development of temperate cereals. Adv Bot Res 34:127–164Google Scholar
  23. Li CD, Ni PX, Francki M, Hunter A, Zhang Y, Schibed D, Li H, Tarr A, Wang J, Cakir M, Yu J, Bellgard M, Lance R, Appels R (2004) Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison. Funct Integr Genomics 4:84–93PubMedCrossRefGoogle Scholar
  24. Lohwasser U, Roder MS, Borner A (2005) QTL mapping of the domestication traits pre-harvest sprouting and dormancy in wheat (Triticum aestivum L.). Euphytica 143:247–249CrossRefGoogle Scholar
  25. Mares D, Mrva K, Cheong J, Williams K, Watson B, Storlie E, Sutherland M, Zou Y (2005) A QTL located on chromosome 4A associated with dormancy in white- and red-grained wheats of diverse origin. Theor Appl Genet 111:1357–1364PubMedCrossRefGoogle Scholar
  26. McCarty DR, Carson CB, Stinard PS, Robertson DS (1989) Molecular analysis of VIVIPAROUS-1: an abscisic acid insensitive mutant of maize. Plant Cell 1:523–532PubMedCentralPubMedGoogle Scholar
  27. McCarty DR, Hattori T, Carson CB, Vasil V, Lazar M, Vasil IK (1991) The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator. Cell 66:895–905PubMedCrossRefGoogle Scholar
  28. McIntosh RA, Devos KM, Dubcovsky J, Rogers WJ, Morris CF, Appels R, Anderson OD (2005) Catalogue of gene symbols for wheat: 2005 supplement. Published online at http://wheat.pw.usda.gov/ggpages/wgc/2005upd.html
  29. McKibbin RS, Wilkinson MD, Bailey PC, Flintham JE, Andrew LM, Lazzeri PA, Gale MD, Lenton JR, Holdworth MJ (2002) Transcripts of Vp-1 homologues are misspliced in modern wheat and ancestral species. Proc Natl Acad Sci USA 99:10203–10208PubMedCrossRefGoogle Scholar
  30. Nakamura S, Toyama T (2001) Isolation of a VP1 homologue from wheat and analysis of its expression in embryos of dormant and non-dormant cultivars. J Exp Bot 52:875–876PubMedCrossRefGoogle Scholar
  31. Nakamura S, Komatsuda T, Miura H (2007) Mapping diploid wheat homologues of Arabidopsis seed ABA signaling genes and QTLs for seed dormancy. Theor Appl Genet 114:1129–1139PubMedCrossRefGoogle Scholar
  32. Nambara E, Keith K, McCourt P, Naito S (1994) Isolation of an internal deletion mutant of the Arabidopsis thaliana ABI3 gene. Plant Cell Physiol 35:509–513PubMedGoogle Scholar
  33. Osa M, Kato K, Mori M, Shindo C, Torada A, Miura H (2003) Mapping QTLs for seed dormancy and the Vp1 homologue on chromosome 3A in wheat. Theor Appl Genet 106:1491–1496PubMedGoogle Scholar
  34. Rohde A, Prinsen E, Rycke De, Engler G, Van MM, Boerjan W (2002) PtABI3 impinges on the growth and differentiation of embryonic leaves during bud set in poplar. Plant Cell 14:1885–1901PubMedCentralPubMedCrossRefGoogle Scholar
  35. Roy JK, Prasad M, Varshney RK (1999) Identification of a microsatellite on chromosomes 6B and a STS on 7D of bread wheat showing an association with pre-harvest sprouting tolerance. Theor Appl Genet 99:336–340CrossRefGoogle Scholar
  36. Shen Q, Gomez-Cadenas A, Zhang P, Walker-Simmons MK, Sheen J, Ho TH (2001) Dissection of abscisic acid signal transduction pathways in barley aleurone layers. Plant Mol Biol l47:437–448CrossRefGoogle Scholar
  37. Sun YW, Jones HD, Yang Y, Dreisigacker S, Li SM, Chen XM, Shewry PR, Xia LQ (2012a) Haplotype analysis of Viviparous-1 gene in CIMMYT elite bread wheat germplasm. Euphytica 186:25–43CrossRefGoogle Scholar
  38. Sun YW, Yang Y, Shewry PR, Jones HD, Xia LQ (2012b) Isolation and characterization of Viviparous-1 haplotypes in wheat related species. Euphytica 188:71–84. doi:10.1007/s10681-012-0659-3 CrossRefGoogle Scholar
  39. Suzuki M, Kao CY, McCarty DR (1997) The conserved B3 domain of VIVIPAROUS1 has a cooperative DNA binding activity. Plant Cell 9:799–807PubMedCentralPubMedGoogle Scholar
  40. Tan MK, Sharp PJ, Lu MQ, Hows N (2006) Genetics of grain dormancy in a white wheat. Aust J Agric Res 57:1157–1165CrossRefGoogle Scholar
  41. Tautz D, Schlotterer C (1994) Simple sequences. Curr Opin Genet Dev 4:832–837PubMedCrossRefGoogle Scholar
  42. Walker-Simmons MK (1988) Enhancement of ABA responsiveness in wheat embryos at higher temperature. Plant Cell Environ 11:769–775CrossRefGoogle Scholar
  43. Xiao SH, Zhang XY, Yan CS, Lin H (2002) Germplasm improvement for pre-harvest sprouting resistance in Chinese white-grained wheat: an overview of the current strategy. Euphytica 126:35–38CrossRefGoogle Scholar
  44. Yang Y, Ma YZ, Xu ZS, Chen XM, He ZH, Yu Z, Wilkinson M, Jones HD, Shewry PR, Xia LQ (2007a) Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance. J Exp Bot 58(11):2863–2871Google Scholar
  45. Yang Y, Zhao XL, Xia LQ, Chen XM, He ZH, Röder MS (2007b) Development and validation of a Viviparous-1 STS marker for pre-harvest sprouting tolerance in Chinese wheats. Theor Appl Genet 115:971–980Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.College of Life ScienceInner Mongolia Agricultural UniversityHohhotChina
  2. 2.Institute of Crop ScienceChinese Academy of Agricultural Sciences (CAAS)BeijingChina
  3. 3.Wheat Research InstituteHeilongjiang Academy of Agricultural SciencesHarbinChina

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