Rapid establishment of introgression lines using cytoplasmic male sterility and a restorer gene in Oryza sativa cv. Nipponbare
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Quantitative trait locus (QTL) analyses have greatly enhanced our understanding of complex traits in rice (Oryza sativa). In parallel, the development of introgression lines has provided a powerful tool for elucidation of complicated genetic networks and identification of QTL. We recently developed a biotron breeding system that allows rapid indoor cultivation of rice plants. The system, however, has two relatively weak points in its application to marker-assisted breeding in rice: first, variation in generation times among cultivars; second, the low number of seeds produced by crosses. To compensate for these weaknesses, we propose utilizing cytoplasmic male sterility (CMS) and restorer (Rf) lines with a cv. Nipponbare genetic background. Through use of the Nipponbare genetic background, rice generation times of 2 months can be achieved regardless of any differences in the genetic background of the donor rice plant. This CMS–Rf system confers a high yield of hybrid seeds, avoids the need for emasculation and precludes accidental crosses. Our results demonstrate that this new methodology can markedly accelerate many different aspects of rice research, especially in functional genomics. The combination of biotron breeding system, early flowering habit and CMS will be of great value for screening candidate genes associated with QTL and for introducing useful QTL into elite cultivars.
KeywordsBiotron breeding system CMS Introgression line MAS Rf gene Rice (Oryza sativa)
We thank Dr. Tomohiko Kazama (Graduate School of Agricultural Science, Tohoku University) for help with marker information and Dr Takahiko Kubo (National Institute of Genetics) for helpful discussions. This study was supported by the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation, Molecular Cloning and Characterization of Agronomically Important Genes of Rice, IPG-0017). T.O. is the recipient of a Grant-in-Aid for JSPS Fellows.
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