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Molecular Breeding

, Volume 33, Issue 4, pp 931–937 | Cite as

Molecular mapping of leaf rust resistance gene LrFun in Romanian wheat line Fundulea 900

  • Lifang Xing
  • Cuifen Wang
  • Xianchun Xia
  • Zhonghu He
  • Wanquan Chen
  • Taiguo Liu
  • Zaifeng Li
  • Daqun Liu
Article

Abstract

Leaf rust, caused by Puccinia triticina, is one of the major wheat diseases worldwide and poses a constant threat to common wheat (Triticum aestivum L.) production and food security. Results from the F2 and F2:3 populations derived from a cross between resistant line Fundulea 900 and susceptible cultivar Thatcher indicated that a single dominant gene, tentatively designated LrFun, conferred resistance to leaf rust. In order to identify other possible genes in Fundulea 900, nine P. triticina pathotypes avirulent on Fundulea 900 were used to inoculate F2:3 families. The results showed that at least two leaf rust resistance genes were present in Fundulea 900. A total of 1,706 pairs of simple sequence repeat (SSR) primers were used to test the parents and resistant and susceptible bulks. Eight polymorphic markers from chromosome 7BL were used for genotyping the F2 and F2:3 populations. LrFun was linked to eight SSR loci on chromosome 7BL. The two closest flanking SSR loci were Xgwm344 and Xwmc70, with genetic distances of 4.4 and 5.7 cM, respectively. At present four leaf rust resistance genes, Lr14a, Lr14b, Lr68 and LrBi16, are located on chromosome 7BL. In a seedling test with 12 P. triticina isolates, the reaction patterns of LrFun were different from those of lines carrying Lr14a, Lr14b and LrBi16. Lr68 is an adult plant resistance gene, and it is different from the seedling resistance gene LrFun. Therefore, we concluded that LrFun is a new leaf rust resistance gene.

Keywords

Common wheat SSR markers Puccinia triticina Leaf rust Molecular mapping 

Notes

Acknowledgments

We are grateful for the critical review of this manuscript by Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney, Australia. The project was supported by National Natural Science Foundation of China (31361140367).

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Lifang Xing
    • 1
  • Cuifen Wang
    • 1
  • Xianchun Xia
    • 2
  • Zhonghu He
    • 2
    • 3
  • Wanquan Chen
    • 4
  • Taiguo Liu
    • 4
  • Zaifeng Li
    • 1
  • Daqun Liu
    • 1
  1. 1.Department of Plant Pathology, College of Plant Protection, Biological Control Center for Plant Diseases and Plant Pests of HebeiAgricultural University of HebeiBaodingChina
  2. 2.Institute of Crop Science, National Wheat Improvement CenterChinese Academy of Agricultural Sciences (CAAS)BeijingChina
  3. 3.International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAASBeijingChina
  4. 4.The State Key Laboratory for Biology of Plant Disease and Insect Pest, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina

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