Molecular Breeding

, Volume 33, Issue 4, pp 931–937 | Cite as

Molecular mapping of leaf rust resistance gene LrFun in Romanian wheat line Fundulea 900

  • Lifang Xing
  • Cuifen Wang
  • Xianchun Xia
  • Zhonghu He
  • Wanquan Chen
  • Taiguo Liu
  • Zaifeng Li
  • Daqun Liu


Leaf rust, caused by Puccinia triticina, is one of the major wheat diseases worldwide and poses a constant threat to common wheat (Triticum aestivum L.) production and food security. Results from the F2 and F2:3 populations derived from a cross between resistant line Fundulea 900 and susceptible cultivar Thatcher indicated that a single dominant gene, tentatively designated LrFun, conferred resistance to leaf rust. In order to identify other possible genes in Fundulea 900, nine P. triticina pathotypes avirulent on Fundulea 900 were used to inoculate F2:3 families. The results showed that at least two leaf rust resistance genes were present in Fundulea 900. A total of 1,706 pairs of simple sequence repeat (SSR) primers were used to test the parents and resistant and susceptible bulks. Eight polymorphic markers from chromosome 7BL were used for genotyping the F2 and F2:3 populations. LrFun was linked to eight SSR loci on chromosome 7BL. The two closest flanking SSR loci were Xgwm344 and Xwmc70, with genetic distances of 4.4 and 5.7 cM, respectively. At present four leaf rust resistance genes, Lr14a, Lr14b, Lr68 and LrBi16, are located on chromosome 7BL. In a seedling test with 12 P. triticina isolates, the reaction patterns of LrFun were different from those of lines carrying Lr14a, Lr14b and LrBi16. Lr68 is an adult plant resistance gene, and it is different from the seedling resistance gene LrFun. Therefore, we concluded that LrFun is a new leaf rust resistance gene.


Common wheat SSR markers Puccinia triticina Leaf rust Molecular mapping 



We are grateful for the critical review of this manuscript by Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney, Australia. The project was supported by National Natural Science Foundation of China (31361140367).


  1. Bassam BJ, Caetano-Anolles G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83PubMedCrossRefGoogle Scholar
  2. Bryan GJ, Collins AJ, Stephenson P, Orry A, Smith JB, Gale MD (1997) Isolation and characterization of microsatellites from hexaploid bread wheat. Theor Appl Genet 94:557–563CrossRefGoogle Scholar
  3. Chen WQ, Qin QM, Chen YL, Yan SB (1998) Virulence dynamics of Puccinia recondita f. sp. tritici in China during 1992–1996. Acta Phytopathol Sin 28:101–106Google Scholar
  4. Chen XC, Li X, Li ZF, Zhang H, Chen H, Gao M, Liu DQ (2010) Molecular mapping of leaf rust resistance gene LrG98 in Chinese wheat line Guizhou98-18. Acta Phytopathol Sin 40:489–494Google Scholar
  5. Dong JG (ed) (2001) Agricultural plant pathology. China Agriculture Press, BeijingGoogle Scholar
  6. Dyck PL, Samborski DJ (1970) The genetics of two alleles for leaf rust resistance at the Lr14 locus in wheat. Can J Genet Cytol 12:689–694Google Scholar
  7. Eriksen L, Afshari F, Christiansen MJ, McIntosh RA, Jahoor A, Wellings CR (2004) Yr32 for resistance to stripe rust present in the wheat cultivar Carstens V. Theor Appl Genet 108:567–575PubMedCrossRefGoogle Scholar
  8. Flor HH (1942) Inheritance of pathogenicity in Melampsora lini. Phytopathology 32:653–669Google Scholar
  9. Helguera M, Vanzetti L, Soria M, Khan IA, Kolmer J, Dubcovsky J (2005) PCR markers for Triticum speltoides leaf rust resistance gene Lr51 and their use to develop isogenic hard red spring wheat lines. Crop Sci 45:728–734CrossRefGoogle Scholar
  10. Herrera-Foessel SA, Singh RP, Huerta-Espino J, William HM, Garcia V, Djurle A, Yuen J (2008) Identification and molecular characterization of leaf rust resistance gene Lr14a in durum wheat. Plant Dis 92:469–473CrossRefGoogle Scholar
  11. Herrera-Foessel SA, Singh RP, Huerta-Espino J, Rosewarne GM, Periyannan SK, Viccars L, Calvo-Salazar V, Lan C, Lagudah ES (2012) Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat. Theor Appl Genet 124:1475–1486PubMedCrossRefGoogle Scholar
  12. Hiebert C, Thomas J, McCallum B (2005) Locating the broad spectrum wheat leaf rust resistance gene Lr52 by a new cytogenetic method. Theor Appl Genet 111:1453–1457CrossRefGoogle Scholar
  13. Khan RR, Bariana HS, Dholakia BB, Naik SV, Lagu MD, Rathjen AJ, Bhavani S, Gupta VS (2005) Molecular mapping of stem and leaf rust resistance in wheat. Theor Appl Genet 111:846–850PubMedCrossRefGoogle Scholar
  14. Knott DR (1989) The wheat-rust breeding for resistance. Monographs on theoretical and applied genetics 12. Springer, BerlinGoogle Scholar
  15. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175CrossRefGoogle Scholar
  16. Law CN, Johnson R (1967) A genetic study of leaf rust resistance in wheat. Can J Genet Cytol 9:805–822Google Scholar
  17. Li X, Li ZF, Li YN, Zhao ZQ, Liu DQ, Wang CF, Gao LJ, Sun DJ (2010a) Genetic analysis and molecular mapping of leaf rust resistance gene in wheat line Xinong 1163-4. Sci Agric Sinica 43:2397–2402Google Scholar
  18. Li ZF, Xia XC, He ZH, Li X, Zhang LJ, Wang HY, Meng QF, Yang WX, Li GQ, Liu DQ (2010b) Seedling and slow rusting resistance to leaf rust in Chinese wheat cultivars. Plant Dis 94:45–53CrossRefGoogle Scholar
  19. Manly FF, Cudmore RH, Meer JM (2001) Map manager QTX, cross platform software for genetic mapping. Mamm Genome 12:930–932PubMedCrossRefGoogle Scholar
  20. McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers DJ, Appels R, Devos KM (2008) Catalogue of gene symbols for wheat. In: 11th International wheat genetics symposium,
  21. McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2009) Catalogue of gene symbols for wheat: 2009 supplement.
  22. McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2010) Catalogue of gene symbols for wheat: 2010 supplement.
  23. McIntosh RA, Dubcovsky J, Rogers WJ, Morris CF, Appels R, Xia XC (2011) Catalogue of gene symbols for wheat: 2011 Supplement. Ann Wheat Newsl 57:303–321Google Scholar
  24. McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2012) Catalogue of gene symbols for wheat: 2012 supplement.
  25. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832PubMedCentralPubMedCrossRefGoogle Scholar
  26. Röder MS, Plaschke J, Konig SU, Borner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246:327–333PubMedCrossRefGoogle Scholar
  27. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedCentralPubMedGoogle Scholar
  28. Roelfs AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concepts and methods of disease management. CIMMYT, Mexico DFGoogle Scholar
  29. Sacco F, Suarez EY, Narango T (1988) Mapping of the leaf rust resistance gene Lr3 on chromosome 6B of Sinvalocho MA wheat. Genome 41:686–690CrossRefGoogle Scholar
  30. Senior ML, Heun M (1993) Mapping maize microsatellites and polymerase chain reaction confirmation of the targeted repeats using a CT primer. Genome 36:884–889PubMedCrossRefGoogle Scholar
  31. Sharp PJ, Kreis M, Shewry PR, Gale MD (1988) Location of β-amylase sequence in wheat and its relatives. Theor Appl Genet 75:286–290CrossRefGoogle Scholar
  32. Singh S, Bowden RL (2011) Molecular mapping of adult-plant race-specific leaf rust resistance gene Lr12 in bread wheat. Mol Breed 28(2):137–142CrossRefGoogle Scholar
  33. Singh S, Franks CD, Huang L, Brown-Guedira GL, Marshall DS, Gill BS, Fritz A (2004) Lr41, Lr39, and a leaf rust resistance gene from Aegilops cylindrica may be allelic and are located on wheat chromosome 2DS. Theor Appl Genet 108:586–591PubMedCrossRefGoogle Scholar
  34. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  35. Sourdille P, Singh S, Cadalen T, Gina L, Brown-Guedira, Gay G, Qi LL, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25Google Scholar
  36. Watson IA, Singh D (1952) The future for rust resistant wheat in Australia. J Aust Inst Agric Sci 18:190–197Google Scholar
  37. Yuan JH, Chen WQ (2010) Leaf rust resistance in 27 diploids and 10 tetraploids of wheat relatives. Acta Agron Sinica 36:1393–1400CrossRefGoogle Scholar
  38. Yuan JH, Chen WQ (2011) Estimate on the effectiveness of main resistant genes for leaf rust in Chinese wheat. J Triticeae Crops 31:994–999Google Scholar
  39. Yuan JH, Liu TG, Chen WQ (2007) Postulation of leaf rust resistance genes in 47 new wheat cultivars at seedling stage. Sci Agric Sin 40:1925–1935Google Scholar
  40. Zhang H, Xia XC, He ZH, Li X, Li ZF, Liu DQ (2011) Molecular mapping of leaf rust resistance gene LrBi16 in Chinese wheat cultivar Bimai 16. Mol Breed 28:527–534CrossRefGoogle Scholar
  41. Zhao XL, Zheng TC, Xia XC, He ZH, Liu DQ, Yang WX, Yin GH, Li ZF (2008) Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou 8425B. Theor Appl Genet 117:1069–1075PubMedCrossRefGoogle Scholar
  42. Zhou HX, Xia XC, He ZH, Li X, Wang CF, Li ZF, Liu DQ (2013a) Molecular mapping of leaf rust resistance gene LrNJ97 in Chinese wheat line Neijiang 977671. Theor Appl Genet 126:2141–2147PubMedCrossRefGoogle Scholar
  43. Zhou Y, Xia XC, He ZH, Li X, Li ZF, Liu DQ (2013b) Fine mapping of leaf rust resistance gene LrZH84 using expressed sequence tag and sequence-tagged site markers, and allelism with other genes on wheat chromosome 1B. Phytopathology 103:169–174PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Lifang Xing
    • 1
  • Cuifen Wang
    • 1
  • Xianchun Xia
    • 2
  • Zhonghu He
    • 2
    • 3
  • Wanquan Chen
    • 4
  • Taiguo Liu
    • 4
  • Zaifeng Li
    • 1
  • Daqun Liu
    • 1
  1. 1.Department of Plant Pathology, College of Plant Protection, Biological Control Center for Plant Diseases and Plant Pests of HebeiAgricultural University of HebeiBaodingChina
  2. 2.Institute of Crop Science, National Wheat Improvement CenterChinese Academy of Agricultural Sciences (CAAS)BeijingChina
  3. 3.International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAASBeijingChina
  4. 4.The State Key Laboratory for Biology of Plant Disease and Insect Pest, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina

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