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Molecular Breeding

, Volume 31, Issue 1, pp 239–246 | Cite as

Improvement of two traditional Basmati rice varieties for bacterial blight resistance and plant stature through morphological and marker-assisted selection

  • Manish K. Pandey
  • N. Shobha RaniEmail author
  • R. M. Sundaram
  • G. S. Laha
  • M. S. Madhav
  • K. Srinivasa Rao
  • Injey Sudharshan
  • Yadla Hari
  • G. S. Varaprasad
  • L. V. Subba Rao
  • Kota Suneetha
  • A. K. P. Sivaranjani
  • B. C. Viraktamath
Short Communication

Abstract

Bacterial blight (BB) is a major production threat to Basmati, the aromatic rice prized for its unique quality. In order to improve the BB resistance of two elite, traditional BB-susceptible Basmati varieties (Taraori Basmati and Basmati 386), we utilized the strategy of limited marker-assisted backcrossing for introgression of two major BB resistance genes, Xa21 and xa13, coupled with phenotype-based selection for improvement of their plant type and yield. Improved Samba Mahsuri, an elite high-yielding, fine-grain-type BB-resistant rice variety served as donor for BB resistance. Backcross-derived improved Basmati lines at BC1F5 possessing a single resistance gene (i.e. either Xa21 or xa13) displayed moderate resistance to BB, while lines possessing both Xa21 and xa13 showed significantly higher levels of resistance. Two-gene pyramid lines (Xa21 + xa13) possessing good grain and cooking quality similar to their respective traditional Basmati parents, short plant stature (<110 cm plant height) and higher grain yield than the recurrent parent(s) were identified and advanced. This work demonstrates the successful application of marker-assisted selection in conjunction with phenotype-based selection for targeted introgression of multiple resistance genes into traditional Basmati varieties along with improvement of their plant stature and yield.

Keywords

Gene pyramiding Bacterial blight resistance Basmati rice Grain quality Marker-assisted selection Morphological selection 

Notes

Acknowledgments

The authors thank the Indian Council of Agricultural Research (ICAR), India for providing financial support. We also thank Mr. M. Vijay Kumar, B. Venkaiah and A. Ashok Reddy for their help in field experiments and grain quality assessment.

Supplementary material

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Supplementary material 1 (DOC 32 kb)
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Supplementary material 2 (DOC 30 kb)
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Supplementary material 3 (DOC 30 kb)
11032_2012_9779_MOESM4_ESM.docx (18 kb)
Supplementary material 4 (DOCX 18 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Manish K. Pandey
    • 1
    • 3
    • 4
  • N. Shobha Rani
    • 1
    Email author
  • R. M. Sundaram
    • 1
  • G. S. Laha
    • 2
  • M. S. Madhav
    • 1
  • K. Srinivasa Rao
    • 1
  • Injey Sudharshan
    • 1
  • Yadla Hari
    • 1
  • G. S. Varaprasad
    • 1
  • L. V. Subba Rao
    • 1
  • Kota Suneetha
    • 1
  • A. K. P. Sivaranjani
    • 1
  • B. C. Viraktamath
    • 1
  1. 1.Crop Improvement Section Directorate of Rice Research (DRR)HyderabadIndia
  2. 2.Crop Protection SectionDirectorate of Rice Research (DRR)HyderabadIndia
  3. 3.Applied Genomics LaboratoryInternational Crops Research Institute for the Semi-Arid TropicsPatancheruIndia
  4. 4.Department of Plant PathologyUniversity of Georgia (UGA)/USDA-ARSTiftonUSA

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