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Molecular Breeding

, Volume 30, Issue 2, pp 1131–1142 | Cite as

Genotyping selection for resistance against tomato yellow leaf curl virus (TYLCV) conferred by Ty-1 and Ty-3 genes in tomato

  • José M. González-Cabezuelo
  • Juan Capel
  • Jesús Abad
  • Diego M. Tomás
  • Rafael Fernández-Muñoz
  • Enrique Moriones
  • Rafael LozanoEmail author
Article

Abstract

The tomato yellow leaf curl virus (TYLCV), transmitted by whitefly, causes major disease losses to tomato crops in tropical and subtropical regions of the world. Several genes conferring resistance to TYLCV, mainly Ty-1 and Ty-3 genes, have been introgressed to cultivated tomato (Solanum lycopersicum) from the wild relative species Solanum chilense. By combining bulked segregant analysis and amplified fragment length polymorphisms (AFLP), several AFLP markers closely linked to Ty-1 and Ty-3 genes were identified from the resistant breeding line TZ841-4. Cloning and sequencing of the selected AFLP fragments allowed us to develop codominant cleaved amplified polymorphic sequence and dominant sequence characterized amplified region markers closely linked to Ty-1. In addition, Ty-3-linked allelic-specific markers have been discriminated by a quantitative real-time PCR protocol. Taken together, these markers constitute useful tools for marker-assisted selection breeding programs to improve genetic resistance to TYLCV, and also to initiate map-based cloning approaches to isolate the resistance genes.

Keywords

Disease resistance TYLCV Marker-assisted selection AFLP SCAR CAPS 

Notes

Acknowledgments

We thank Fernando J. Yuste-Lisbona for critical reading of the manuscript and Eduardo R. Bejarano for providing the infectious clone of TYLCV-IL. This work was supported by grants from Ministerio de Ciencia e Innovación (AGL2007-66062-C02-01 and 05-0004) and Junta de Andalucía (IDEA 130278D). We also thank Campus de Excelencia Internacional Agroalimentario, CeiA3 for providing financial support.

Supplementary material

11032_2012_9701_MOESM1_ESM.jpg (49 kb)
Supplementary material 1 (JPG 49 kb)
11032_2012_9701_MOESM2_ESM.doc (41 kb)
Electronic Supplementary Material Table 1. AFLP primer combinations used in the BSA approach (DOC 41 kb)
11032_2012_9701_MOESM3_ESM.doc (33 kb)
Electronic Supplementary Material Table 2. Universality of markers linked to Ty-1 and Ty-3 resistance genes (DOC 33 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • José M. González-Cabezuelo
    • 1
  • Juan Capel
    • 1
  • Jesús Abad
    • 2
  • Diego M. Tomás
    • 3
  • Rafael Fernández-Muñoz
    • 3
  • Enrique Moriones
    • 3
  • Rafael Lozano
    • 1
    Email author
  1. 1.Departamento de Biología Aplicada (Genética), Edificio CITE II-B, Centro de Investigación en Biotecnología AgroalimentariaUniversidad de AlmeríaAlmeríaSpain
  2. 2.Zeta SeedsAlmeríaSpain
  3. 3.Instituto de Hortofruticultura Subtropical Mediterránea La MayoraUMA-CSICMálagaSpain

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