Molecular Breeding

, Volume 30, Issue 2, pp 1089–1096 | Cite as

Identification of a new resistance gene Pi-Da(t) from Dacca6 against rice blast fungus (Magnaporthe oryzae) in Jin23B background

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Abstract

Rice blast, caused by the fungal pathogen Magnaporthe oryzae, severely threatens rice production worldwide. A new resistance gene, Pi-Da(t), was found in Dacca6, a local upland rice variety from the Philippines. It was mapped into a region between RM5529 and RM211 on chromosome 2, where no blast resistance gene has been reported, by bulk segregant analysis (BSA) in a BC1F2 population from a cross between Dacca6 and Jin23B. The presence of Pi-Da(t) in Jin23B background, an elite parental line preferred for its good grain quality and widely adopted in China’s three-line hybrid rice breeding program over the past 20 years, was verified by BSA and graphical genotyping with additional eight BC1F2 bulks. This work presents an example of combining gene mapping work and gene introgression with BSA and graphical genotyping methods in a backcross (BC) breeding scheme. Both the resistant Jin23B line and the linked markers will provide useful information and materials for marker-assisted breeding against blast disease in rice.

Keywords

Pi-Da(t) Jin23B Blast resistance BSA Graphical genotyping BC breeding Oryza sativa L. 
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Notes

Acknowledgments

This research was funded by the Chinese National Natural Science Foundation (31000700), the Natural Science Foundation (2009J1092) of Fujian Province, the Fujian Provincial Key Sci-Tech Project (2008NZ02), the “948” project (2011-G2B) from the Chinese Ministry of Agriculture, the “Green Super Rice” project (2010AA101803;2010AA101806) from the Chinese Ministry of Science & Technology, and a grant (51587) from the Bill & Melinda Gates Foundation.

Supplementary material

11032_2011_9695_MOESM1_ESM.ppt (202 kb)
Supplementary material 1 (PPT 201 kb)
11032_2011_9695_MOESM2_ESM.xls (394 kb)
Supplementary material 2 (XLS 394 kb)
11032_2011_9695_MOESM3_ESM.doc (42 kb)
Supplementary material 3 (DOC 42 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • B. H. Shi
    • 1
  • J. H. Zhang
    • 1
  • Y. M. Zheng
    • 2
  • Y. Q. Liu
    • 2
  • C. M. Vera Cruz
    • 3
  • T. Q. Zheng
    • 4
  • M. F. Zhao
    • 2
  1. 1.College of Life SciencesFujian Normal UniversityFuzhouChina
  2. 2.Rice Research InstituteFujian Academy of Agricultural SciencesFuzhouChina
  3. 3.International Rice Research InstituteMetro ManilaPhilippines
  4. 4.Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural SciencesBeijingChina

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