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Molecular Breeding

, Volume 30, Issue 2, pp 1037–1044 | Cite as

Heavy-ion beam irradiation is an effective technique for reducing major allergens in peanut seeds

  • Cerrone S. Cabanos
  • Hiroki Katayama
  • Hiroyuki Urabe
  • Chikara Kuwata
  • Yuri Murota
  • Tomoko Abe
  • Yutaka Okumoto
  • Nobuyuki Maruyama
Article

Abstract

Heavy-ion beam irradiation is an effective technique for mutation breeding to produce new cultivars. Heavy-ion beams have high linear energy transfer capable of breaking the double-stranded DNA molecules, thus inducing stable knockout mutants. Here, we report the first application of this technology to produce hypoallergenic peanut lacking major allergens, Ara h 2 and 3, from the Japanese Nakateyutaka variety. After irradiation with either N or C heavy-ion beams at a dose of 100 Gy, seventeen knockout mutants from 11,335 screened M2 seeds were obtained, eight of which lacked either one of the two isoforms of Ara h 2, and the other nine lacked one of the isoforms of Ara h 3. This result indicates that heavy-ion beam irradiation is a powerful means of producing knockout hypoallergenic peanuts.

Keywords

Peanut Heavy-ion beam Allergen Hypoallergenic 

Abbreviations

HIBI

Heavy-ion beam irradiation

RIBF

RIKEN Nishina Center Radioactive-Isotope Beam Factory

IUIS

International Union of Immunological Societies

SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

Notes

Acknowledgments

We thank Prof. Reiko Urade (Kyoto University) for warm encouragement. The first author also wishes to thank the Japanese Government (Monbukagakusho:MEXT) for scholarship support.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Cerrone S. Cabanos
    • 1
  • Hiroki Katayama
    • 1
  • Hiroyuki Urabe
    • 1
  • Chikara Kuwata
    • 2
  • Yuri Murota
    • 3
  • Tomoko Abe
    • 4
  • Yutaka Okumoto
    • 5
  • Nobuyuki Maruyama
    • 1
  1. 1.Laboratory of Food Quality Design and Development, Graduate School of AgricultureKyoto UniversityUji, KyotoJapan
  2. 2.Chiba Prefectural Agriculture and Forestry Research Center, Upload Crops Breeding LaboratoryChibaJapan
  3. 3.Chiba Prefectural Agriculture and Forestry Research Center, Biotechnology LaboratoryChibaJapan
  4. 4.Radiation Biology TeamRIKEN Nishina Center, RIKENHirosawa, WakoJapan
  5. 5.Laboratory of Plant Breeding, Graduate School of AgricultureKyoto UniversitySakyo-ku, KyotoJapan

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