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Molecular Breeding

, Volume 30, Issue 1, pp 569–582 | Cite as

QTL mapping for fiber quality traits across multiple generations and environments in upland cotton

  • Fu-Ding Sun
  • Jian-Hong Zhang
  • Shu-Fang Wang
  • Wan-Kui Gong
  • Yu-Zhen Shi
  • Ai-Ying Liu
  • Jun-Wen Li
  • Ju-Wu Gong
  • Hai-Hong Shang
  • You-Lu Yuan
Article

Abstract

Identification of quantitative trait loci (QTL) for fiber quality traits that are stable across multiple generations and environments could facilitate marker-assisted selection for improving cotton strains. In the present study, F2, F2:3, and recombinant inbred lines (RILs, F 6:8 ) populations derived from an upland cotton (Gossypium hirsutum L.) cross between strain 0-153, which has excellent fiber quality, and strain sGK9708, a commercial transgenic cultivar, were constructed for QTL tagging of fiber quality. We used 5,742 simple sequence repeat primer pairs to screen for polymorphisms between the two parent strains. Linkage maps of F2 and RILs were constructed, containing 155 and 190 loci and with a total map distance of 959.4 centimorgans (cM) and 700.9 cM, respectively. We screened fiber quality QTL across multiple generations and environments through composite interval mapping of fiber quality data. Specifically, we studied F2 and F2:3 family lines from Anyang (Henan Province) in 2003 and 2004 and RILs in Anyang in 2007 and Anyang, Quzhou (Hebei Province), and Linqing (Shandong Province) in 2008. We identified 50 QTL for fiber quality: 10 for fiber strength, 10 for fiber length, 10 for micronaire, eight for fiber uniformity, and 12 for fiber elongation. Nine of these fiber quality QTL were identified in F2, F2:3 and RILs simultaneously. Two QTL for fiber strength on chromosomes C7 and C25 were detected in all three generations and all four environments and explained 16.67–27.86% and 9.43–21.36% of the phenotypic variation, respectively. These stable QTL for fiber quality traits could be used for marker assisted selection.

Keywords

Fiber quality Multiple generations QTL Upland cotton (Gossypium hirsutum L.) 

Notes

Acknowledgments

This study was financially supported by the 973 Project (2010CB126006), and Hi-tech Research and Development Program of China (2006AA100105).

References

  1. An CF, Jenkins JN, Wu JX, Guo YF, McCarty JC (2010) Use of fiber and fuzz mutants to detect QTL for yield components, seed, and fiber traits of upland cotton. Euphytica 172:21–34CrossRefGoogle Scholar
  2. Austin DF, Lee M (1996) Comparative mapping in F2:3 and F6:7 generations of quantitative trait loci for grain yield and yield components in maize. Theor Appl Genet 92:817–826CrossRefGoogle Scholar
  3. Chen L, Zhang ZS, Hu MC, Wang W, Zhang J, Liu DJ, Zheng J, Zheng FM, Ma J (2008) Genetic linkage map construction and QTL mapping for yield and fiber quality in Upland Cotton(Gossypium hirsutum L.). Acta Agron Sin 34:1199–1205CrossRefGoogle Scholar
  4. Chen H, Qian N, Guo WZ, Song QP, Li BC, Deng FJ, Dong CG, Zhang TZ (2009) Using three overlapped RILs to dissect genetically clustered QTL for fiber strength on Chro.D8 in Upland cotton. Theor Appl Genet 119:605–612PubMedCrossRefGoogle Scholar
  5. Cowen NM (1988) The use of replicated progenies in marker-based mapping of QTLs. Theor Appl Genet 75:857–862Google Scholar
  6. Guo WZ, Zhang TZ, Ding YZ, Zhu YC, Shen XL, Zhu XF (2005) Molecular marker assisted selection and pyramiding of two QTLs for fiber strength in upland cotton. Acta Genet Sin 32:1275–1285PubMedGoogle Scholar
  7. Guo WZ, Cai CP, Wang CB, Han ZG, Song XL, Wang K, Niu XW, Wang C, Lu KY, Shi B, Zhang TZ (2007) A microsatellite-based, gene-rich linkage map reveals genome structure, function, and evolution in Gossypium. Genetics 176:527–541PubMedCrossRefGoogle Scholar
  8. Han ZG, Wang CB, Song XL, Guo WZ, Gou JY, Li CH, Chen XY, Zhang TZ (2006) Characteristics, development and mapping of Gossypium hirsutum derived EST-SSRs in allotetraploid cotton. Theor Appl Genet 112:430–439PubMedCrossRefGoogle Scholar
  9. He DH, Lin ZX, Zhang XL, Nie YC, Guo XP, Zhang XY, Li W (2007) QTL mapping for economic traits based on a dense genetic map of cotton with PCR-based markers using the interspecific cross of Gossypium hirsutum × Gossypium barbadense. Euphytica 153:181–197CrossRefGoogle Scholar
  10. Jiang C, Wright RJ, El-Zik KM, Paterson AH (1998) Polyploid formation created unique avenues for response to selection in Gossypium (cotton). Proc Natl Acad Sci USA 95:4419–4424PubMedCrossRefGoogle Scholar
  11. Knapp SJ, Bridges WC (1990) Using molecular markers to estimate quantitative trait locus parameters; power and genetic variances for unreplicated and replicated progeny. Genetics 126:769–777PubMedGoogle Scholar
  12. Kohel RJ, Yu J, Park YH, Lazo GR (2001) Molecular mapping and characterization of traits controlling fiber quality in cotton. Euphytica 121:163–172CrossRefGoogle Scholar
  13. Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175CrossRefGoogle Scholar
  14. Lacape JM, Nguyen TB, Brigitte C, Courtois B, Belot JL, Giband M, Gourlot JP, Gawryziak G, Roques S, Hau B (2005) QTL analysis of cotton quality using multiple Gossypium hirsutum × Gossypium barbadense backcross generations. Crop Sci 45:123–140Google Scholar
  15. Lacape JM, Jacobs J, Arioli T, Derijcker R, Forestier-Chrion N, Llewellyn D, Jean J, Thomas E, Viot C (2009) A new interspecific, Gossypium hirsutum × G. barbadense, RIL population: towards a unified consensus linkage map of tetraploid cotton. Theor Appl Genet 119:281–292PubMedCrossRefGoogle Scholar
  16. Mei M, Syed NH, Gao W, Thaxton PM, Smith CW, Stelly DM, Chen ZJ (2004) Genetic mapping and QTL analysis of fiber-related traits in cotton (Gossypium). Theor Appl Genet 108:280–291PubMedCrossRefGoogle Scholar
  17. Murigneux A, Baud S, Beckert M (1993) Molecular and morphological evaluation of doubled-haploid lines in maize 2. Comparison with single-seed descent lines. Theor Appl Genet 87:278–287CrossRefGoogle Scholar
  18. Park YH, Alabadt MS, Ulloa M, Sicker B, Wilkins TA, Yu J, Stelly DM, Kohel RJ, El-Shihy OM, Cantrell RG (2005) Genetic mapping of new cotton fiber loci using EST-derived microsatellites in an interspecific recombinant inbred line cotton population. Mol Genet Genom 274:428–441CrossRefGoogle Scholar
  19. Paterson AH, Brubaker CL, Wendel JF (1993) A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis. Plant Mol Biol Rep 11:122–127CrossRefGoogle Scholar
  20. Paterson AH, Saranga Y, Menz M, Jiang CX, Wright RJ (2003) QTL analysis of genotype by environment interaction affecting cotton fiber quality. Theor Appl Genet 106:384–396PubMedGoogle Scholar
  21. Qin HD, Guo WZ, Zhang YM, Zhang TZ (2008) QTL mapping of yield and fiber traits based on a four-way cross population in Gossypium hirsutum L. Theor Appl Genet 117:883–894PubMedCrossRefGoogle Scholar
  22. Rong JK, Abbey C, Bowers JE, Brubaker CL, Chang C, Chee PW, Delmonte TA, Ding X, Garza JJ, Marler BS, Park C, Pierce GJ, Rainey KM, Rastogi VK, Schulze SR, Tronlinde NL, Wendel JF, Wilkins TA, Wing RA, Wright RJ, Zhao X, Zhu L, Paterson AH (2004) A 3347-locus genetic recombination map of sequence-tagged sites reveals features of genome organization, transmission and evolution of cotton (Gossypium). Genetics 166:389–417PubMedCrossRefGoogle Scholar
  23. Shappley ZW, Jenkins JN, Zhu J, McCarty JC (1998) Quantitative trait loci associated with agronomic and fiber traits of upland cotton. J Cotton Sci 2:153–163Google Scholar
  24. Shen XL, Guo WZ, Zhu XF, Yuan YL, Yu JZ, Kohel RJ, Zhang TZ (2005) Molecular mapping of QTLs for qualities in three diverse lines in Upland cotton using SSR markers. Mol Breed 15:169–181CrossRefGoogle Scholar
  25. Shen XL, Guo WZ, Lu QX, Zhu XF, Yuan YL, Zhang TZ (2007) Genetic mapping of quantitative trait loci for fiber quality and yield trait by RIL approach in Upland cotton. Euphytica 155:371–380CrossRefGoogle Scholar
  26. Shi YZ, Liu AY, Li JW, Shao YH, Yuan YL (2007) The major QTLs linked to fiber strength for cotton breeding program by molecular marker assisted selection. Mol Plant Breed 5:521–527Google Scholar
  27. Song XL, Wang K, Guo WZ, Zhang J, Zhang TZ (2005) A comparison of genetic maps constructed from haploid and BC1 mapping populations from the same crossing between Gossypium hirsutum L. and Gossypium barbadense L. Genome 48:378–390PubMedCrossRefGoogle Scholar
  28. Ulloa M, Meredith WR (2000) Genetic linkage map and QTL analysis of agronomic and fiber quality traits in an intraspecific population. J of Cotton Sci 4:161–170Google Scholar
  29. Ulloa M, Meredith WR, Shappley ZW, Kahler AL (2002) RFLP genetic linkage maps from four F2:3 populations and a joinmap of Gossypium hirsutum L. Theor Appl Genet 104:200–208PubMedCrossRefGoogle Scholar
  30. Ulloa M, Saha S, Jenkins JN, Meredith WR, McCarty JC, Stelly MD (2005) Chromosomal assignment of RFLP linkage groups harboring important QTLs on an intraspecific cotton (Gossypium hirsutum L.) joinmap. J Hered 96:132–144PubMedCrossRefGoogle Scholar
  31. Van Ooijen JW, Voorrips RE (2001) JoinMap 3.0, software for the calculation of genetic linkage maps. Plant Research International, WageningenGoogle Scholar
  32. Voorrips RE (2006) MapChart 2.2: software for the graphical presentation of linkage maps and QTLs. Plant Research International, WageningenGoogle Scholar
  33. Wan Q, Zhang ZS, Hu MC, Chen L, Liu DJ, Chen X, Wang W, Zheng J (2007) T1 locus in cotton is the candidate gene affecting lint percentage, fiber quality and spiny bollworm (Earias spp.) resistance. Euphytica 158:241–247CrossRefGoogle Scholar
  34. Wang BH, Guo WZ, Zhu XF, Wu YT, Huang NT, Zhang TZ (2006a) QTL mapping of fiber quality in an elite hybrid derived-RIL population of upland cotton. Euphytica 152:367–378CrossRefGoogle Scholar
  35. Wang S, Basten CJ, Zeng ZB (2006b) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh http://www.statgen.ncsu.edu/qtlcart/WQTLCart.htm
  36. Wu JX, Gutierrez OA, Jenkins JN, McCarty JC, Zhu J (2009) Quantitative analysis and QTL mapping for agronomic and fiber traits in an RI population of upland cotton. Euphytica 165:231–245CrossRefGoogle Scholar
  37. Yi CX, Wang YC, Guo WZ, Zhu XF, Zhang TZ (2004) Pyramid breeding by marker-assisted recurrent selection in Upland Cotton. Acta Agron Sin 30:680–685Google Scholar
  38. Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468PubMedGoogle Scholar
  39. Zhang J, Guo WZ, Zhang TZ (2002) Molecular linkage map of allotetraploid (Gossypium hirsutum L. × Gossypium barbadense L.) with a haploid population. Theor Appl Genet 105:1166–1174PubMedCrossRefGoogle Scholar
  40. Zhang TZ, Yuan YL, Yu J, Guo WZ, Kohel RJ (2003) Molecular tagging of a major QTL for fiber strength in Upland cotton and its marker-assisted selection. Theor Appl Genet 106:262–268PubMedGoogle Scholar
  41. Zhang ZS, Xiao YH, Luo M, Li XB, Luo XY, Hou L, Li DM, Pei Y (2005) Construction of a genetic linkage map and QTL analysis of fiber-related traits in upland cotton (Gossypium hirsutum L.). Euphytica 144:91–99CrossRefGoogle Scholar
  42. Zhang ZS, Hu MC, Zhang J, Liu DJ, Zheng J, Zhang K, Wang W, Wan Q (2009) Construction of a comprehensive PCR-based marker linkage map and QTL mapping for fiber quality traits in upland cotton (Gossypium hirsutum L.). Mol Breed 24:49–61CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Fu-Ding Sun
    • 1
  • Jian-Hong Zhang
    • 1
  • Shu-Fang Wang
    • 1
  • Wan-Kui Gong
    • 1
  • Yu-Zhen Shi
    • 1
  • Ai-Ying Liu
    • 1
  • Jun-Wen Li
    • 1
  • Ju-Wu Gong
    • 1
  • Hai-Hong Shang
    • 1
  • You-Lu Yuan
    • 1
  1. 1.Cotton Research Institute, State Key Laboratory of Cotton BiologyChinese Academy of Agricultural SciencesAnyangChina

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