Molecular Breeding

, Volume 30, Issue 1, pp 239–250 | Cite as

Genetic linkage maps of the chromosomal regions associated with apomictic and sexual modes of reproduction in Cenchrus ciliaris

  • Chandra Bhan Yadav
  • Anuj
  • Suresh Kumar
  • M. G. Gupta
  • Vishnu Bhat
Article
First Online:
Received:
Accepted:

Abstract

Cenchrus ciliaris reproduces by apomixis, an asexual mode of reproduction through seeds. Genetic analysis of apomixis in this species revealed that this trait is dominant and that a chromosomal region of more than 11 Mb controls this trait, which is hemizygous, heterochromatic and recombinationally suppressed. A novel F2 mapping population comprising 86 individuals segregating for apomictic and sexual modes of reproduction, generated after crossing a new set of obligate apomictic and sexual parents (IG-96-3108 and IG-96-443), was used in this study to identify a large number of amplified fragment length polymorphism (AFLP) and sequence characterized amplified region (SCAR) markers linked to these traits. Out of 180 polymorphic AFLP markers, 42 and 29 markers associated with apomixis and sexuality were mapped around Apo and Sexual loci, respectively. Markers 20G, 18G and 19G showed close linkage to Apo locus at map distance of only 1.1 cM, while 12FS, 4HS and 12b showed tight linkage to Sexual locus at map distance of 1.7 cM. Markers clustered around Apo and Sexual loci on either side. A large number of recombining AFLP markers were mapped around both loci, indicating a minor role of suppression of recombination. Four anchor markers from earlier studies also clustered around Apo locus, validating the present genetic linkage map. In addition, seven and one SCAR markers closely linked to Apo and Sexual loci were also developed, which could be used for fine mapping of the loci.

Keywords

Apomixis AFLP Genetic linkage map Sexual reproduction Cenchrus 
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Notes

Acknowledgments

Authors wish to gratefully acknowledge the Department of Biotechnology, Govt. of India, New Delhi for the liberal funding to undertake a major part of the research work mentioned in this paper. We also wish to thank Director, IGFRI, Jhansi, UP, India for providing the plant material for research. We are grateful to Dr. Johan W. Van Ooijen, The Netherlands for helping us with data analysis for mapping using JoinMap software. We are grateful to the University of Delhi for the R&D grant to carry out part of this work. Authors are grateful to Dr. Avinash Kumar, T.H. Dar and Dr. Deepmala Sehgal for their help during the experimentation.

Supplementary material

11032_2011_9614_MOESM1_ESM.pptx (83 kb)
Linkage groups obtained for (a) male parent (IG-96-3108) and (b) female parent (IG-96-443) based on linkage analysis using JoinMap 2.0 software (PPTX 83 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Chandra Bhan Yadav
    • 1
  • Anuj
    • 1
  • Suresh Kumar
    • 2
    • 3
  • M. G. Gupta
    • 2
  • Vishnu Bhat
    • 1
  1. 1.Department of BotanyUniversity of DelhiDelhiIndia
  2. 2.Division of Crop ImprovementIndian Grassland and Fodder Research InstituteJhansiIndia
  3. 3.Department of Horticulture and Landscape ArchitecturePurdue UniversityWest LafayetteUSA

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