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Molecular Breeding

, Volume 30, Issue 1, pp 79–92 | Cite as

Relationships between grain protein content and grain yield components through quantitative trait locus analyses in a recombinant inbred line population derived from two elite durum wheat cultivars

  • A. BlancoEmail author
  • G. Mangini
  • A. Giancaspro
  • S. Giove
  • P. Colasuonno
  • R. Simeone
  • A. Signorile
  • P. De Vita
  • A. M. Mastrangelo
  • L. Cattivelli
  • A. Gadaleta
Article

Abstract

Grain protein content (GPC) in durum wheat (Triticum turgidum var. durum) is negatively correlated with grain yield. To evaluate possible genetic interrelationships between GPC and grain yield per spike, thousand-kernel weight and kernel number per spike, quantitative trait loci (QTL) for GPC were mapped using GPC-adjusted data in a covariance analysis on yield components. Phenotypic data were evaluated in a segregating population of 120 recombinant inbred lines derived from crossing the elite cultivars Svevo and Ciccio. The material was tested at five environments in southern Italy. QTL were determined by composite interval mapping based on the Svevo × Ciccio linkage map described in Gadaleta et al. (2009) and integrated with DArT markers. The close relationship between GPC and yield components was reflected in the negative correlation between the traits and in the reduction of variance when GPC values were adjusted to yield components. Ten independent genomic regions involved in the expression of GPC were detected, six of which were associated with QTL for one or more grain yield components. QTL alleles with increased GPC effects were associated with QTL alleles with decreased effects on one or more yield component traits, or vice versa (i.e. the allelic effects were in opposite direction). Four QTL for GPC showed always significant effects, and these QTL should represent genes that influence GPC independently from variation in the yield components. Such genes are of special interest in wheat breeding since they would allow an increase in GPC without a concomitant decrease in grain yield.

Keywords

Wheat Grain protein content Yield components Molecular markers QTL mapping 

Notes

Acknowledgments

The research project was supported by grants from Ministero dell’Istruzione, dell’Università e della Ricerca, projects ‘FISR’ and ‘AGROGEN’.

Supplementary material

11032_2011_9600_MOESM1_ESM.doc (28 kb)
Supplementary material 1 (DOC 28 kb)
11032_2011_9600_MOESM2_ESM.doc (206 kb)
Supplementary material 2 (DOC 205 kb)
11032_2011_9600_MOESM3_ESM.doc (30 kb)
Supplementary material 3 (DOC 29 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • A. Blanco
    • 1
    Email author
  • G. Mangini
    • 1
  • A. Giancaspro
    • 1
  • S. Giove
    • 1
  • P. Colasuonno
    • 1
  • R. Simeone
    • 1
  • A. Signorile
    • 1
  • P. De Vita
    • 2
  • A. M. Mastrangelo
    • 2
  • L. Cattivelli
    • 2
    • 3
  • A. Gadaleta
    • 1
  1. 1.Department of Environmental and Agro-Forestry Biology and Chemistry, Sect. Genetic and Plant BreedingUniversity of BariBariItaly
  2. 2.CRA—Cereal Research CentreFoggiaItaly
  3. 3.CRA—Genomic Research CentreFiorenzuola d’ArdaItaly

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