Development of breeder-friendly markers for selection of MIPS1 mutations in soybean
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Mutations in the d-myo-inositol 3-phosphate synthase 1 gene (MIPS1) in soybean [Glycine max (L.) Merr.] cause modifications to seed phosphorus and carbohydrate content that improve the nutritional value of food and feed. Molecular markers are an efficient tool for breeding MIPS1 mutant germplasm due to reduced seed germination and field emergence potential. An F2 population segregating for the MIPS1 mutation found in experimental soybean line V99-5089 was used to develop breeder-friendly markers. Markers were validated in 88 advanced lines from 9 diverse pedigrees. Ten potential simple sequence repeat (SSR) markers, located on Gm11, from the new BARCSOYSSR_1.0 database were tested and four were polymorphic. BARCSOY_11_1495 was 93–97% effective for selecting the mutation. A KBiosciences Competitive Allele Specific PCR (KASPar) assay was developed to select directly for the V99-5089-derived MIPS1 single nucleotide polymorphism (SNP) mutation. The KASPar assay is simple and cost-effective compared to other SNP genotyping assays. The MIPS1 mutation in V99-5089 is likely to have occurred spontaneously. We describe a method of DNA extraction in soybean using a Geno/Grinder for fast and easy tissue maceration.
KeywordsBARCSOYSSR_1.0 Simple sequence repeat (SSR) KBiosciences Competitive Allele Specific PCR (KASPar) Low phytate (LP) d-myo-inositol 3-phosphate synthase 1 gene (MIPS1) Single nucleotide polymorphism (SNP) Soybean (Glycine max)
We thank the United Soybean Board for funding projects 8234, “Development of low phytate soybeans using genomic tools” and 8227, “The use of genomics to improve soybean meal digestibility and food quality”, that supported this research.
- Burleson SA, Shang C, Rosso ML, Paupin LM, Rainey KM (2011) A high-throughput colorimetric method for selection of soybean phytate concentration. Crop Sci (submitted)Google Scholar
- Chen W, Mingus J, Mammadov J, Backlund JE, Greene T, Thompson S, Kumpatla S (2010) KASPar: a simple and cost-effective system for SNP genotyping. In: Proceedings of plant and animal genomes XVII conference, San Diego, US, p 194Google Scholar
- Huhn MR (2003) Inheritance of soluble oligosacharide content of soybean seeds. M.S. Virginia Polytechnic Institute State University, Blacksburg, VAGoogle Scholar
- Maupin LM, Rainey KM (2011) Improving emergence in modified phosphorus composition soybeans: genotypes, germplasm, environements, and selection. Crop Sci. (in press)Google Scholar
- Maupin LM, Rosso ML, Shang C, Burleson S, Rainey KM (2011b) Evaluation of emergence and phosphorus content of an early generation soybean population with modified phosphorus and sugar composition. Crop Sci SubmittedGoogle Scholar
- Saghai Maroof MA, Buss GR (2008) Low phytic acid, low stachyose, high sucrose soybean lines. US Patent 0199591 A1Google Scholar
- Sebastian S, Kerr PS, Pearlstein RW, Hitz WD (2000) Soybean germplasm with novel genes for improved digestibility. In: Drackley JK (ed) Soy in animal nutrition. Federation of Animal Sci Soc, Savoy, pp 56–74Google Scholar
- Shoemaker RC, Grant D, Olson T, Warren WC, Wing R, Yu Y, Kim H, Cregan P, Joseph B, Futrell-Griggs F, Nelson W, Davito J, Walker J, Wallis J, Kremitski C, Scheer D, Clifton SW, Graves T, Nguyen H, Wu X, Luo M, Dvorak J, Nelson R, Cannon S, Tomkins J, Shmutz J, Stacey G, Jackson S (2008) Microsatellite discovery from BAC end sequences and genetic mapping to anchor the soybean physical and genetic maps. Genome 51:294–302PubMedCrossRefGoogle Scholar