Molecular Breeding

, Volume 29, Issue 2, pp 379–397 | Cite as

EST contig-based SSR linkage maps for Malus × domestica cv Royal Gala and an apple scab resistant accession of M. sieversii, the progenitor species of domestic apple

  • Aide Wang
  • Herb Aldwinckle
  • Philip Forsline
  • Dorrie Main
  • Gennaro Fazio
  • Susan Brown
  • Kenong Xu
Article

Abstract

Malus sieversii is a progenitor species of domestic apple M. × domestica. Using population “GMAL 4595” of 188 individuals derived from a cross of Royal Gala × PI 613988 (apple scab resistant, M. sieversii), 287 SSR (simple sequence repeats) loci were mapped. Of these SSRs, 80 are published anchors and 207 are newly developed EST (expressed sequence tag) contig-based SSRs, representing 1,630 Malus EST accessions in GenBank. Putative gene functions of these EST contigs are diverse, including regulating plant growth, development and response to environmental stresses. Among the 80 published SSRs, 18 are PI 613988 specific, 38 are common and 24 are Royal Gala specific. Out of the 207 newly developed EST contig-based SSRs, 79 are PI 613988 specific, 45 are common and 83 are Royal Gala specific. These results led to the construction of a M. sieversii map (1,387.0 cM) of 180 SSR markers and a Royal Gala map (1,283.4 cM) of 190 SSR markers. Mapping of scab resistance was independently conducted in two subsets of population “GMAL 4595” that were inoculated with Ventura inaequalis races (1) and (2), respectively. In combination with the two major resistance reactions Chl (chlorotic lesions) and SN (stellate necrosis) to each race, four subsets of resistance data, i.e., Chl/race (1), SN/race (1), Chl/race (2) and SN/race (2), were constituted and analyzed, leading to four resistance loci mapped to the linkage group 2 of PI 613988; SNR1 (stellate necrosis resistance to race (1)) and SNR2 are tightly linked in a region of known scab resistance genes, and ChlR1 (Chlorotic lesion resistance to race (1)) and ChlR2 are also linked tightly but in a region without known scab resistance genes. The utility of the two linkage maps, the new EST contig-based markers and M. sieversii as sources of apple scab resistance are discussed.

Keywords

Malus sieversii Linkage map Expressed sequence tag (EST) Simple sequence repeats (SSR) Apple scab resistance 

Supplementary material

11032_2011_9554_MOESM1_ESM.doc (423 kb)
Supplementary material 1 (DOC 423 kb)

References

  1. Beckerman J, Chatfield J, Draper E (2009) A 33-year evaluation of resistance and pathogenicity in the apple scab-crabapples pathosystem. Hort Sci 44:599–608Google Scholar
  2. Bus VGM, Laurens FND, van de Weg WE, Rusholme RL, Rikkerink EHA, Gardiner SE, Bassett HCM, Kodde LP, Plummer KM (2005a) The Vh8 locus of a new gene-for-gene interaction between Venturia inaequalis and the wild apple Malus sieversii is closely linked to the Vh2 locus in Malus pumila R12740-7A. New Phytol 166:1035–1049PubMedCrossRefGoogle Scholar
  3. Bus VGM, Rikkerink EHA, Van de Weg WE, Rusholme RL, Gardiner SE, Bassett HCM, Kodde LP, Parisi L, Laurens FND, Meulenbroek EJ, Plummer KM (2005b) The Vh2 and Vh4 scab resistance genes in two differential hosts derived from Russian apple R12740-7A map to the same linkage group of apple. Mol Breed 15:103–116CrossRefGoogle Scholar
  4. Bus V, Rikkerink E, Aldwinckle HS, Caffier V, Durel CE, Gardiner S, Gessler C, Groenwold R, Laurens F, Cam BL, Luby J, Meulenbroek B, Kellerhals M, Parisi L, Patocchi A, Plummer K, Schouten HJ, Tartarini S, van de Weg WE (2009) A proposal for the nomenclature of Venturia inaequalis races. Acta Hort 814:739–746Google Scholar
  5. Bus V, Bassett H, Bowatte D, Chagné D, Ranatunga C, Ulluwishewa D, Wiedow C, Gardiner S (2010) Genome mapping of an apple scab, a powdery mildew and a woolly apple aphid resistance gene from open-pollinated mildew immune selection. Tree Genet Genomes 6:477–487CrossRefGoogle Scholar
  6. Celton J-M, Chagné D, Tustin SD, Terakami S, Nishitani C, Yamamoto T, Gardiner SE (2009a) Update on comparative genome mapping between Malus and Pyrus. BMC Res Notes 2:182PubMedCrossRefGoogle Scholar
  7. Celton JM, Tustin DS, Chagne D, Gardiner SE (2009b) Construction of a dense genetic linkage map for apple rootstocks using SSRs developed from Malus ESTs and Pyrus genomic sequences. Tree Genet Genomes 5:93–107CrossRefGoogle Scholar
  8. Chagne D, Gasic K, Crowhurst RN, Han Y, Bassett HC, Bowatte DR, Lawrence TJ, Rikkerink EHA, Gardiner SE, Korban SS (2008) Development of a set of SNP markers present in expressed genes of the apple. Genomics 92:353–358PubMedCrossRefGoogle Scholar
  9. Chiu JC, Brenner ED, DeSalle R, Nitabach MN, Holmes TC, Coruzzi GM (2002) Phylogenetic and expression analysis of the glutamate-receptor-like gene family in Arabidopsis thaliana. Mol Biol Evol 19:1066–1082PubMedCrossRefGoogle Scholar
  10. Conner PJ, Brown SK, Weeden NF (1997) Randomly amplified polymorphic DNA-based genetic linkage maps of three apple cultivars. J Am Soc Hort Sci 122:350–359Google Scholar
  11. Cullings KW (1992) Design and testing of a plant-specific PCR primer for ecological and evolutionary studies. Mol Ecol 1:233–240CrossRefGoogle Scholar
  12. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  13. Erdin N, Tartarini S, Broggini GAL, Gennari F, Sansavini S, Gessler C, Patocchi A (2006) Mapping of the apple scab-resistance gene Vb. Genome 49:1238–1245PubMedCrossRefGoogle Scholar
  14. Fernandez-Fernandez F, Evans KM, Clarke JB, Govan CL, James CM, Maric S, Tobutt KR (2008) Development of an STS map of an interspecific progeny of Malus. Tree Genet Genomes 4:469–479CrossRefGoogle Scholar
  15. Forsline PL, Hummer KE (2007) Fruit exploration supported by the National Plant Germplasm System, 1980 to 2004. Hort Sci 42:200–202Google Scholar
  16. Forsline PL, Aldwinckle HS, Dickson EE, Luby JJ, Hokanson SC (2003) Collection, maintenance, characterization and utilization of wild apples of Central Asia. In: Janick J (ed) Horticultural reviews. Wiley, New York, pp 1–61Google Scholar
  17. Galli P, Broggini G, Kellerhals M, Gessler C, Patocchi A (2010a) High-resolution genetic map of the Rvi15 (Vr2) apple scab resistance locus. Mol Breed 26:561–572CrossRefGoogle Scholar
  18. Galli P, Patocchi A, Broggini GAL, Gessler C (2010b) The Rvi15 (Vr2) apple scab resistance locus contains three TIR-NBS-LRR genes. Mol Plant Microbe Interact 23:608–617PubMedCrossRefGoogle Scholar
  19. Gasic K, Gonzalez DO, Thimmapuram J, Liu L, Malnoy M, Gong G, Han Y, Vodkin LO, Aldwinckle HS, Carroll NJ, Orvis KS, Goldsbrough P, Clifton S, Pape D, Fulton L, Martin J, Theising B, Wisniewski ME, Fazio G, Feltus FA, Korban SS (2009a) Comparative analysis and functional annotation of a large expressed sequence tag collection of apple. Plant Genome 2:23–38CrossRefGoogle Scholar
  20. Gasic K, Han YP, Kertbundit S, Shulaev V, Iezzoni AF, Stover EW, Bell RL, Wisniewski ME, Korban SS (2009b) Characteristics and transferability of new apple EST-derived SSRs to other Rosaceae species. Mol Breed 23:397–411CrossRefGoogle Scholar
  21. Gessler C, Patocchi A, Sansavini S, Tartarini S, Gianfranceschi L (2006) Venturia inaequalis resistance in apple. Crit Rev Plant Sci 25:473–503CrossRefGoogle Scholar
  22. Gharghani A, Zamani Z, Talaie A, Oraguzie NC, Fatahi R, Hajnajari H, Wiedow C, Gardiner SE (2009) Genetic identity and relationships of Iranian apple (Malus × domestica Borkh.) cultivars and landraces, wild Malus species and representative old apple cultivars based on simple sequence repeat (SSR) marker analysis. Genet Resour Crop Evol 56:829–842CrossRefGoogle Scholar
  23. Grattapaglia D, Sederoff R (1994) Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers. Genetics 137:1121–1137PubMedGoogle Scholar
  24. Guerin F, Le Cam B (2004) Breakdown of the scab resistance gene Vf in apple leads to a founder effect in populations of the fungal pathogen Venturia inaequalis. Phytopathology 94:364–369PubMedCrossRefGoogle Scholar
  25. Gygax M, Gianfranceschi L, Liebhard R, Kellerhals M, Gessler C, Patocchi A (2004) Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii. Theor Appl Genet 109:1702–1709PubMedCrossRefGoogle Scholar
  26. Han YP, Chagne D, Gasic K, Rikkerink EHA, Beever JE, Gardiner SE, Korban SS (2009) BAC-end sequence-based SNPs and Bin mapping for rapid integration of physical and genetic maps in apple. Genomics 93:282–288PubMedCrossRefGoogle Scholar
  27. Harris SA, Robinson JP, Juniper BE (2002) Genetic clues to the origin of the apple. Trends Genet 18:426–430PubMedCrossRefGoogle Scholar
  28. Hemmat M, Weeden NF, Manganaris AG, Lawson DM (1994) Molecular marker linkage map for apple. J Hered 85:4–11PubMedGoogle Scholar
  29. Igarashi M, Abe Y, Hatsuyama Y, Ueda T, Fukasawa-Akada T, Kon T, Kudo T, Sato T, Suzuki M (2008) Linkage maps of the apple (Malus × domestica Borkh.) cvs ‘Ralls Janet’ and ‘Delicious’ include newly developed EST markers. Mol Breed 22:95–118CrossRefGoogle Scholar
  30. Jung S, Staton M, Lee T, Blenda A, Svancara R, Abbott A, Main D (2008) GDR (Genome Database for Rosaceae): integrated web-database for Rosaceae genomics and genetics data. Nucleic Acids Res 36:D1034–D1040PubMedCrossRefGoogle Scholar
  31. Juniper BE (2007) The mysterious origin of the sweet apple—on its way to a grocery counter near you, this delicious fruit traversed continents and mastered coevolution. Am Sci 95:44–51Google Scholar
  32. Juniper BE, Watkins R, Harris SA (1999) The origin of the apple. Acta Hort 484:27–33Google Scholar
  33. Kim SA, Kwak JM, Jae SK, Wang MH, Nam HG (2001) Overexpression of the AtGluR2 gene encoding an arabidopsis homolog of mammalian glutamate receptors impairs calcium utilization and sensitivity to ionic stress in transgenic plants. Plant Cell Physiol 42:74–84PubMedCrossRefGoogle Scholar
  34. Korban SS, Wannarat W, Rayburn CM, Tatum TC, Rayburn AL (2009) Genome size and nucleotypic variation in Malus germplasm. Genome 52:148–155PubMedCrossRefGoogle Scholar
  35. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175CrossRefGoogle Scholar
  36. Lalli D, Wisniewski M, Norelli JL, Malnoy M, Pindo M, Fazio G, Forsline P, Aldwinckle H, Gardiner S, Chagné D (2010) Mapping Malus sieversii: a valuable genetic resource for apple breeding. Plant & Animal Genomes XVIII Conference, San Diego, CA, p 206Google Scholar
  37. Li J, Zhu SH, Song XW, Shen Y, Chen HM, Yu J, Yi KK, Liu YF, Karplus VJ, Wu P, Deng XW (2006) A rice glutamate receptor-like gene is critical for the division and survival of individual cells in the root apical meristem. Plant Cell 18:340–349PubMedCrossRefGoogle Scholar
  38. Liebhard R, Gianfranceschi L, Koller B, Ryder CD, Tarchini R, Van de Weg E, Gessler C (2002) Development and characterisation of 140 new microsatellites in apple (Malus × domestica Borkh.). Mol Breed 10:217–241CrossRefGoogle Scholar
  39. Liebhard R, Koller B, Gianfranceschi L, Gessler C (2003) Creating a saturated reference map for the apple (Malus × domestica Borkh.) genome. Theor Appl Genet 106:1497–1508PubMedGoogle Scholar
  40. Luby J, Forsline P, Aldwinckle H, Bus V, Geibel M (2001) Silk road apples—collection, evaluation, and utilization of Malus sieversii from Central Asia. Hort Sci 36:225–231Google Scholar
  41. Luby J, Hokanson K, Forsline P, Aldwinckle H, Gustafson H, Gardiner S, Bone R, Cook M, Bus V (2006) Evaluation of horticulturally elite Malus sieversii germplasm for apple scab resistance genes using phenotypic and marker-based screening. In: The 3rd international Rosaceae genomics conference, NapierGoogle Scholar
  42. Maliepaard C, Alston FH, van Arkel G, Brown LM, Chevreau E, Dunemann F, Evans KM, Gardiner S, Guilford P, van Heusden AW, Janse J, Laurens F, Lynn JR, Manganaris AG, den Nijs APM, Periam N, Rikkerink E, Roche P, Ryder C, Sansavini S, Schmidt H, Tartarini S, Verhaegh JJ, Vrielink-van Ginkel M, King GJ (1998) Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers. Theor Appl Genet 97:60–73CrossRefGoogle Scholar
  43. Malnoy M, Xu M, Borejsza-Wysocka E, Korban SS, Aldwinckle HS (2008) Two receptor-like genes, Vfa1 and Vfa2, confer resistance to the fungal pathogen Venturia inaequalis inciting apple scab disease. Mol Plant Microbe Interact 21:448–458PubMedCrossRefGoogle Scholar
  44. N’Diaye A, de Weg WEV, Kodde LP, Koller B, Dunemann F, Thiermann M, Tartarini S, Gennari F, Durel CE (2008) Construction of an integrated consensus map of the apple genome based on four mapping populations. Tree Genet Genomes 4:727–743CrossRefGoogle Scholar
  45. Naik S, Hampson C, Gasic K, Bakkeren G, Korban SS (2006) Development and linkage mapping of E-STS and RGA markers for functional gene homologues in apple. Genome 49:959–968PubMedCrossRefGoogle Scholar
  46. Newcomb RD, Crowhurst RN, Gleave AP, Rikkerink EHA, Allan AC, Beuning LL, Bowen JH, Gera E, Jamieson KR, Janssen BJ, Laing WA, McArtney S, Nain B, Ross GS, Snowden KC, Souleyre EJF, Walton EF, Yauk YK (2006) Analyses of expressed sequence tags from apple. Plant Physiol 141:147–166PubMedCrossRefGoogle Scholar
  47. Patocchi A, Bigler B, Koller B, Kellerhals M, Gessler C (2004) Vr(2): a new apple scab resistance gene. Theor Appl Genet 109:1087–1092PubMedCrossRefGoogle Scholar
  48. Patocchi A, Walser M, Tartarini S, Broggini GAL, Gennari F, Sansavini S, Gessler C (2005) Identification by genome scanning approach (GSA) of a microsatellite tightly associated with the apple scab resistance gene Vm. Genome 48:630–636PubMedCrossRefGoogle Scholar
  49. Patocchi A, Fernández-Fernández F, Evans K, Gobbin D, Rezzonico F, Boudichevskaia A, Dunemann F, Stankiewicz-Kosyl M, Mathis-Jeanneteau F, Durel C, Gianfranceschi L, Costa F, Toller C, Cova V, Mott D, Komjanc M, Barbaro E, Kodde L, Rikkerink E, Gessler C, van de Weg W (2009a) Development and test of 21 multiplex PCRs composed of SSRs spanning most of the apple genome. Tree Genet Genomes 5:211–223CrossRefGoogle Scholar
  50. Patocchi A, Frei A, Frey JE, Kellerhals M (2009b) Towards improvement of marker assisted selection of apple scab resistant cultivars: Venturia inaequalis virulence surveys and standardization of molecular marker alleles associated with resistance genes. Mol Breed 24:337–347CrossRefGoogle Scholar
  51. Pilcher RLR, Celton JM, Gardiner SE, Tustin DS (2008) Genetic markers linked to the dwarfing trait of apple rootstock ‘Malling 9’. J Am Soc Hort Sci 133:100–106Google Scholar
  52. Richards CM, Volk GM, Reeves PA, Reilley AA, Henk AD, Forsline PL, Aldwinckle HS (2009a) Selection of stratified core sets representing wild apple (Malus sieversii). J Am Soc Hort Sci 134:228–235Google Scholar
  53. Richards CM, Volk GM, Reilley AA, Henk AD, Lockwood DR, Reeves PA, Forsline PL (2009b) Genetic diversity and population structure in Malus sieversii, a wild progenitor species of domesticated apple. Tree Genet Genomes 5:339–347CrossRefGoogle Scholar
  54. Robinson JP, Harris SA, Juniper BE (2001) Taxonomy of the genus Malus Mill. (Rosaceae) with emphasis on the cultivated apple, Malus domestica Borkh. Plant Syst Evol 226:35–58CrossRefGoogle Scholar
  55. Sargent D, Marchese A, Simpson D, Howad W, Fernández-Fernández F, Monfort A, Arús P, Evans K, Tobutt K (2009) Development of “universal” gene-specific markers from Malus spp. cDNA sequences, their mapping and use in synteny studies within Rosaceae. Tree Genet Genomes 5:133–145CrossRefGoogle Scholar
  56. Segura V, Durel CE, Costes E (2009) Dissecting apple tree architecture into genetic, ontogenetic and environmental effects: QTL mapping. Tree Genet Genomes 5:165–179CrossRefGoogle Scholar
  57. Silfverberg-Dilworth E, Matasci CL, Van de Weg WE, Van Kaauwen MPW, Walser M, Kodde LP, Soglio V, Gianfranceschi L, Durel CE, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning thess apple (Malus × domestica Borkh.) genome. Tree Genet Genomes 2:202–224CrossRefGoogle Scholar
  58. Soriano JM, Joshi SG, van Kaauwen M, Noordijk Y, Groenwold R, Henken B, van de Weg WE, Schouten HJ (2009) Identification and mapping of the novel apple scab resistance gene Vd3. Tree Genet Genomes 5:475–482CrossRefGoogle Scholar
  59. Sun L, John Bukovac M, Forsline P, van Nocker S (2009) Natural variation in fruit abscission-related traits in apple (Malus). Euphytica 165:55–67CrossRefGoogle Scholar
  60. Tapken D, Hollmann M (2008) Arabidopsis thaliana glutamate receptor ion channel function demonstrated by ion pore transplantation. J Mol Biol 383:36–48PubMedCrossRefGoogle Scholar
  61. Tatum TC, Stepanovic S, Biradar DP, Rayburn AL, Korban SS (2005) Variation in nuclear DNA content in Malus species and cultivated apples. Genome 48:924–930PubMedCrossRefGoogle Scholar
  62. Troggio M, Zharkikh A, Pindo M, Baldi P, Costa F, Chagné D, Micheletti D, Coppola G, Cestaro A, Lasserre P, Crowhurst RN, Bus V, Magnago P, Komjanc M, Lespinasse Y, Salvi S, Durel C-E, Gardiner SE, Velasco R (2010) A sequence-anchored integrated genetic linkage map for apple, Malus × domestica Borkh. Plant & Animal Genomes XVIII Conference, San DiegoGoogle Scholar
  63. Van Ooijen JW, Voorrips RE (2001) JoinMap® 3.0, Software for the calculation of genetic linkage maps. Plant Research International, WageningenGoogle Scholar
  64. Velasco R, Zharkikh A, Affourtit J, Dhingra A, Cestaro A, Kalyanaraman A, Fontana P, Bhatnagar SK, Troggio M, Pruss D, Salvi S, Pindo M, Baldi P, Castelletti S, Cavaiuolo M, Coppola G, Costa F, Cova V, Dal Ri A, Goremykin V, Komjanc M, Longhi S, Magnago P, Malacarne G, Malnoy M, Micheletti D, Moretto M, Perazzolli M, Si-Ammour A, Vezzulli S, Zini E, Eldredge G, Fitzgerald LM, Gutin N, Lanchbury J, Macalma T, Mitchell JT, Reid J, Wardell B, Kodira C, Chen Z, Desany B, Niazi F, Palmer M, Koepke T, Jiwan D, Schaeffer S, Krishnan V, Wu C, Chu VT, King ST, Vick J, Tao Q, Mraz A, Stormo A, Stormo K, Bogden R, Ederle D, Stella A, Vecchietti A, Kater MM, Masiero S, Lasserre P, Lespinasse Y, Allan AC, Bus V, Chagne D, Crowhurst RN, Gleave AP, Lavezzo E, Fawcett JA, Proost S, Rouze P, Sterck L, Toppo S, Lazzari B, Hellens RP, Durel C-E, Gutin A, Bumgarner RE, Gardiner SE, Skolnick M, Egholm M, Van de Peer Y, Salamini F, Viola R (2010) The genome of the domesticated apple (Malus × domestica Borkh.). Nat Genet 42:833–839PubMedCrossRefGoogle Scholar
  65. Volk GM, Richards CM, Reilley AA, Henk AD, Forsline PL, Aldwinckle HS (2005) Ex situ conservation of vegetatively propagated species: development of a seed-based core collection for Malus sieversii. J Am Soc Hort Sci 130:203–210Google Scholar
  66. Volk GM, Richards CM, Henk AD, Reilley A, Miller DD, Forsline PL (2009) Novel diversity identified in a wild apple population from the Kyrgyz Republic. Hort Sci 44:516–518Google Scholar
  67. Voorrips RE (2002) MapChart: Software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78PubMedCrossRefGoogle Scholar
  68. Wang D, Shi J, Carlson SR, Cregan PB, Ward RW, Diers BW (2003) A low-cost, high-throughput polyacrylamide gel electrophoresis system for genotyping with microsatellite DNA markers. Crop Sci 43:1828–1832CrossRefGoogle Scholar
  69. Williams EB, Kuc J (1969) Resistance in Malus to Venturia Inaequalis. Annu Rev Phytopathol 7:223–246CrossRefGoogle Scholar
  70. Wisniewski M, Bassett C, Norelli J, Macarisin D, Artlip T, Gasic K, Korban S (2008) Expressed sequence tag analysis of the response of apple (Malus × domestica ‘Royal Gala’) to low temperature and water deficit. Physiol Plant 133:298–317PubMedCrossRefGoogle Scholar
  71. Yao L, Zheng X, Cai D, Gao Y, Wang K, Cao Y, Teng Y (2010) Exploitation of Malus EST-SSRs and the utility in evaluation of genetic diversity in Malus and Pyrus. Genet Resour Crop Evol 57:841–851CrossRefGoogle Scholar
  72. Yepes LM, Aldwinckle HS (1993) Pathogenesis of Venturia inaequalis on shoot-tip cultures and on greenhouse-grown apple cultivars. Phytopathology 83:1155–1162CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Aide Wang
    • 1
  • Herb Aldwinckle
    • 2
  • Philip Forsline
    • 3
  • Dorrie Main
    • 4
  • Gennaro Fazio
    • 3
  • Susan Brown
    • 1
  • Kenong Xu
    • 1
  1. 1.Department of HorticultureCornell University, New York State Agricultural Experiment StationGenevaUSA
  2. 2.Department of Plant Pathology and Plant-Microbe BiologyCornell University, New York State Agricultural Experiment StationGenevaUSA
  3. 3.USDA-ARS, PGRUCornell University, New York State Agricultural Experiment StationGenevaUSA
  4. 4.Department of Horticulture and Landscape ArchitectureWashington State UniversityPullmanUSA

Personalised recommendations