Molecular Breeding

, Volume 29, Issue 2, pp 361–370

A molecular marker linked to the mollis gene conferring soft-seediness for marker-assisted selection applicable to a wide range of crosses in lupin (Lupinus angustifolius L.) breeding

Article

Abstract

To broaden the gene pool of domesticated commercial cultivars of narrow-leafed lupin (Lupinus angustifolius L.), wild accessions are used as parents in crossing in lupin breeding. Among the progenies from wild × domesticated (W × D) crosses, the soft-seediness gene mollis is the most difficult domestication gene to be selected by conventional breeding methods, where molecular marker-assisted selection (MAS) is highly desirable. MAS in plant breeding requires markers to be cost-effective and high-throughput, and be applicable to a wide range of crosses in a breeding program. In this study, representative plants from an F8 recombinant inbred line (RIL) population derived from a W × D cross, together with four cultivars and four wild types, were used in DNA fingerprinting by microsatellite-anchored fragment length polymorphisms (MFLP). Two co-dominant MFLP polymorphisms were identified as candidate markers linked to the mollis gene, and one of the candidate markers was selected and converted into a co-dominant, sequence-specific PCR marker. This marker, designated MoLi, showed a perfect match with phenotypes of seed coat permeability on a segregating population consisting of 115 F8 RILs, confirming the close genetic linkage to the mollis gene. Validation tests showed that the banding pattern of marker MoLi is consistent with all the 25 historical and current commercial cultivars released in Australia, and is consistent with mollis genotypes in 119 of the 125 accessions in the Australian L. angustifolius core collection. Marker MoLi provides a cost-effective way to select the mollis gene in a wide range of W × D crosses in lupin breeding.

Keywords

Lupinus angustifolius Soft-seediness Molecular marker Marker-assisted selection (MAS) MFLP 

References

  1. Boersma JG, Buirchell BJ, Sivasithamparam K, Yang H (2007) Development of a PCR marker tightly linked to mollis, the gene that controls seed dormancy in Lupinus angustifolius L. Plant Breed 126:612–616CrossRefGoogle Scholar
  2. Clements JC, Buirchell BJ, Yang H, Smith PMC, Sweetingham MW, Smith CG (2005) Chapter 9, Lupin. In: Singh RJ, Jauhar PP (eds) Genetic resources, chromosome engineering, and crop improvement. Vol 1, Grain legumes. CRC Press, Florida, pp 231–323Google Scholar
  3. Forbes I, Wells HD (1968) Hard and soft seediness in blue lupine, Lupinus angustifolius L.: inheritance and phenotype classification. Crop Sci 8:195–197CrossRefGoogle Scholar
  4. Gladstones JS (1970) Lupins as crop plants. Field Crop Abstr 23:123–148Google Scholar
  5. Gupta PK, Varshney RK, Sharma PC, Ramesh B (1999) Molecular markers and their applications in wheat breeding. Plant Breed 118:369–390CrossRefGoogle Scholar
  6. Holland JB (2004) Implementation of molecular markers for quantitative traits in breeding programs—challenges and opportunities. Proceedings of the 4th international crop science congress, 26 Sep–1 Oct 2004. Brisbane, Australia (www.cropscience.org.au)
  7. Manly KF, Cudmore RH Jr, Meer JM (2001) MapManager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932PubMedCrossRefGoogle Scholar
  8. Mikolajczyk J (1966) Genetic studies in Lupinus angustifolius. 2. Inheritance of some morphological characters in blue lupine. Genet Polon 7:153–180Google Scholar
  9. Quinlivan BJ (1966) The relationship between temperature fluctuations and the softening of hard seeds of some legume species. Aust J Agric Res 12:1009–1022CrossRefGoogle Scholar
  10. Quinlivan BJ (1968) The softening of hard seeds of sand-plain lupin (Lupinus varius L.). Aust J Agric Res 19:507–515CrossRefGoogle Scholar
  11. Sharp PJ, Johnston S, Brown G, McIntosh RA, Pallotta M, Carter M, Bariana HS, Khatkar S, Lagudah ES, Singh RP, Khairallah M, Potter R, Jones MGK (2001) Validation of molecular markers for wheat breeding. Aust J Agric Res 52:1357–1366CrossRefGoogle Scholar
  12. Snape JW (2004) Challenges of integrating conventional breeding and biotechnology: a personal view! Proceedings of the 4th international crop science congress, 26 Sep–1 Oct 2004. Brisbane, Australia (www.cropscience.org.au)
  13. Staub JE, Serquen FC (1996) Genetic markers, map construction, and their application in plant breeding. Hort Sci 31:729–740Google Scholar
  14. Stefanova KT, Buirchell BJ (2010) Multiplicative mixed models for genetic gain assessment in lupin breeding. Crop Sci 50:880–891CrossRefGoogle Scholar
  15. Vos P, Hogers R, Bleeker M, Reijans M, Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414PubMedCrossRefGoogle Scholar
  16. Yang H, Sweetingham MW, Cowling WA, Smith PMC (2001) DNA fingerprinting based on microsatellite-anchored fragment length polymorphisms, and isolation of sequence-specific PCR markers in lupin (Lupinus angustifolius L.). Mol Breed 7:203–209CrossRefGoogle Scholar
  17. Yang H, Shankar M, Buirchell BJ, Sweetingham MW, Caminero C, Smith PMC (2002) Development of molecular markers using MFLP linked to a gene conferring resistance to Diaporthe toxica in narrow-leafed lupin (Lupinus angustifolius L.). Theor Appl Genet 105:265–270PubMedCrossRefGoogle Scholar
  18. Yang H, Boersma JG, You M, Buirchell BJ, Sweetingham MW (2004) Development and implementation of a sequence-specific PCR marker linked to a gene conferring resistance to anthracnose disease in narrow-leafed lupin (Lupinus angustifolius L.). Mol Breed 14:145–151CrossRefGoogle Scholar
  19. Yang H, Renshaw D, Thomas G, Buirchell B, Sweetingham M (2008) A strategy to develop molecular markers applicable to a wide range of crosses for marker-assisted selection in plant breeding: a case study on anthracnose disease resistance in lupin (Lupinus angustifolius L.). Mol Breed 21:473–483CrossRefGoogle Scholar
  20. You M, Boersma JG, Buirchell BJ, Sweetingham MW, Siddique KHM, Yang H (2005) A PCR-based molecular marker applicable for marker-assisted selection for anthracnose disease resistance in lupin breeding. Cell Mol Biol Lett 10:123–134PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Xin Li
    • 1
  • Bevan Buirchell
    • 2
  • Guijun Yan
    • 1
  • Huaan Yang
    • 2
  1. 1.School of Plant Biology and Institute of Agriculture, Faculty of Natural and Agricultural SciencesThe University of Western AustraliaCrawleyAustralia
  2. 2.Department of Agriculture and Food Western AustraliaSouth PerthAustralia

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