Molecular Breeding

, Volume 28, Issue 4, pp 585–596 | Cite as

Development and genetic mapping of microsatellite markers from whole genome shotgun sequences in Brassica oleracea

  • Haitao Li
  • Xun Chen
  • Yuan Yang
  • Jinsong Xu
  • Jianxun Gu
  • Jie Fu
  • Xiaoju Qian
  • Shunchang Zhang
  • Jiangsheng Wu
  • Kede Liu
Original Paper

Abstract

The availability of whole genome shotgun sequences (WGSs) in Brassica oleracea provides an unprecedented opportunity for development of microsatellite or simple sequence repeat (SSR) markers for genome analysis and genetic improvement in Brassica species. In this study, a total of 56,465 non-redundant SSRs were identified from the WGSs in B. oleracea, with dinucleotide repeats being the most abundant, followed by tri-, tetra- and pentanucleotide repeats. From these, 1,398 new SSR markers (designated as BoGMS) with repeat length ≥25 bp were developed and used to survey polymorphisms with a panel of six rapeseed varieties, which is the largest number of SSR markers developed for the C genome in a single study. Of these SSR markers, 752 (69.5%) showed polymorphism among the six varieties. Of these, 266 markers that showed clear scorable polymorphisms between B. napus varieties No. 2127 and ZY821 were integrated into an existing B. napus genetic linkage map. These new markers are preferentially distributed on the linkage groups in the C genome, and significantly increased the number of SSR markers in the C genome. These SSR markers will be very useful for gene mapping and marker-assisted selection of important agronomic traits in Brassica species.

Keywords

Brassica oleracea Whole genome shotgun sequences Microsatellites Simple sequence repeats Brassica napus Linkage map 

Supplementary material

11032_2010_9509_MOESM1_ESM.xls (412 kb)
Supplementary material 1 (XLS 412 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Haitao Li
    • 1
  • Xun Chen
    • 1
  • Yuan Yang
    • 1
  • Jinsong Xu
    • 1
  • Jianxun Gu
    • 1
  • Jie Fu
    • 1
  • Xiaoju Qian
    • 1
  • Shunchang Zhang
    • 1
  • Jiangsheng Wu
    • 1
  • Kede Liu
    • 1
  1. 1.National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan)Huazhong Agricultural UniversityWuhanChina

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