Molecular Breeding

, Volume 28, Issue 4, pp 453–462 | Cite as

Mapping two major effect grain dormancy QTL in rice

  • Bingyue Lu
  • Kun Xie
  • Chunyan Yang
  • Songfeng Wang
  • Xi Liu
  • Long Zhang
  • Ling JiangEmail author
  • Jianmin WanEmail author


The intrachromosomal positions of the two grain dormancy quantitative trait loci (QTL) qSdn-1 (chromosome 1) and qSdn-5 (chromosome 5) were obtained from the segregation analysis of the advanced backcross populations derived from the cross between rice (Oryza sativa L.) cultivars N22 (indica) and Nanjing35 (japonica). Marker-assisted selection (MAS) was applied to select derivatives carrying one or both of qSdn-1 and qSdn-5 in a genetic background which was nearly isogenic to Nanjing35. An analysis of dormancy in the BC4F2 population allowed qSdn-1 to be located between the simple sequence repeat (SSR) markers RM11669 and RM1216; the QTL explained 24.58% of the overall phenotypic variation and the most closely linked marker was RM11694. qSdn-5 was mapped between RM480 and RM3664, and explained 17.58% of the overall phenotypic variation. The SSR locus RM19080 mapped within 0.4 cM of qSdn-5. No epistasis was observed between qSdn-1 and qSdn-5. The mean germination rates of lines containing qSdn-1, qSdn-5 and both qSdn-1 and qSdn-5 were 7.9, 11.1 and 6.1%, respectively, whereas that of the check line lacking both QTL was 86.3%. The SSR loci linked most tightly to qSdn-1 and qSdn-5 are suitable for MAS for reduced pre-harvest sprouting in rice. The dormancy of both qSdn-1 and qSdn-5 could be readily broken by a 7-day post-harvest treatment at 50°C.


Dormancy Advanced backcross population Epistatic interaction Dry heat treatment Rice (Oryza sativa L.) 



Advanced backcross






Near-isogenic line


Polymerase chain reaction


Pre-harvest sprouting


Quantitative trait loci of seed dormancy in N22


Quantitative trait loci of seed dormancy in Nanjing35


Quantitative trait loci


Simple sequence repeat



This research was supported by grants from the National Natural Science Foundation of China (No. 30471120, 30671246), 863 Program (projects 2009AA101101) of China, the Jiangsu Cultivar Development Program (projects BE2009301-3 and BE2008352), and the 111 Project (B08025).

Supplementary material

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Bingyue Lu
    • 1
  • Kun Xie
    • 1
  • Chunyan Yang
    • 1
  • Songfeng Wang
    • 1
  • Xi Liu
    • 1
  • Long Zhang
    • 1
  • Ling Jiang
    • 1
    Email author
  • Jianmin Wan
    • 1
    • 2
    Email author
  1. 1.National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjingChina
  2. 2.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijingChina

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