Advertisement

Molecular Breeding

, Volume 28, Issue 3, pp 391–402 | Cite as

Genetic dissection of early-season cold tolerance in sorghum (Sorghum bicolor (L.) Moench)

  • Gloria Burow
  • John J. Burke
  • Zhanguo Xin
  • Cleve D. Franks
Article

Abstract

Soil temperatures at 15°C or below limit germination and seedling establishment for warm season cereal crops such as sorghum (Sorghum bicolor (L.) Moench) during early-season planting. To better understand the genetics of early-season cold tolerance in sorghum, mapping of quantitative trait loci (QTL) associated with germination, emergence and vigor using a recombinant inbred mapping population was carried out. A mapping population consisting of 171 F7–F8 recombinant inbred lines (RILs) derived from the cross between RTx430 (cold-sensitive) and PI610727 (cold-tolerant) was developed and a genetic map was constructed using 141 microsatellites or simple sequence repeat (SSR) markers. The RILs were evaluated for cold and optimal temperature germinability in the laboratory, field emergence, and seedling vigor in two locations during early-season planting. Two or more QTL were detected for all traits, except for seedling vigor, with only one QTL was detected in the population. A QTL for cold germinability (Germ 12-2.1) showed the highest LOD value and was also associated with optimal germinability. One of the QTL for field emergence, Fearlygerm-9.3, a contribution from PI610727, was found significant in both locations used for the study. This study showed alignment of QTL in SBi1 (Fearlygerm-1.2 and FGerm30-1.2) with previously reported QTL associated with late field emergence identified from a different mapping population. This indicates that PI617027 shares some common loci with other known early-season cold-tolerant sorghum germplasm but also harbors novel QTL that could be useful in introgression of enhanced laboratory germination and early-season field emergence.

Keywords

Sorghum bicolor L. Early-season cold tolerance Quantitative trait loci (QTL) mapping RIL population Microsatellite markers 

Notes

Acknowledgments

The authors would like to thank Ms. Halee Hughes and Mr. Charlie Woodfin for their technical support in this project.

Supplementary material

11032_2010_9491_MOESM1_ESM.ppt (62 kb)
Figure S1. Mean air and soil temperature during the early-season cold planting conducted from April 1 to April 30, 2009. (PPT 62 kb)
11032_2010_9491_MOESM2_ESM.ppt (72 kb)
Figure S2. Molecular map developed in the study from the population RTx430 × PI610727. (PPT 72 kb)

References

  1. Association of Official Seed Analysts (AOSA) (1999) Rules for testing seeds. AOSA, Las CrucesGoogle Scholar
  2. Bacon RK, Cantrell RP, Axtell JD (1986) Selection for seedling cold tolerance in grain sorghum. Crop Sci 26:900–903CrossRefGoogle Scholar
  3. Basten C, Weir B, Zeng Z (2003) QTL cartographer version 1.17. North Carolina State University, RaleighGoogle Scholar
  4. Burow G, Franks C, Acosta-Martinez V, Xin Z (2009) Molecular mapping and characterization of profuse wax (bloom) and enhanced cuticular features of Sorghum (Sorghum bicolor (L.) Moench). Theor Appl Genet 118:423–431PubMedCrossRefGoogle Scholar
  5. Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971PubMedGoogle Scholar
  6. Cisse N, Ejeta G (2003) Genetic variation and relationships among seedling vigor traits in sorghum. Crop Sci 43:824–828CrossRefGoogle Scholar
  7. Doggett H (1988) Sorghum, 2nd edn. Wiley, New YorkGoogle Scholar
  8. Franks C, Burow G, Burke J (2006) A comparison of U.S. and Chinese sorghum germplasm for early season cold tolerance. Crop Sci 46:1371–1376CrossRefGoogle Scholar
  9. Fujino K, Sekiguchi H, Sato T, Kiuchi H, Nonue Y, Takeuichi Y, Ando T, Lin SY, Yano M (2004) Mapping of quantitative trait loci controlling low-temperature germinability in rice (Oryza sativa L.). Theor Appl Genet 108:794–799PubMedCrossRefGoogle Scholar
  10. Fujino K, Sekiguchi H, Matsuda Y, Sugimoto K, Ono K, Yano M (2008) Molecular identification of a major quantitative trait locus, qLTG3–1, controlling low-temperature germinability in rice. PNAS 105:12623–12628PubMedCrossRefGoogle Scholar
  11. Kim JS, Klein PE, Klein RR, Price HJ, Mullet JE, Stelly DM (2005) Chromosome identification and nomenclature of Sorghum bicolor. Genetics 169:1169–1173PubMedCrossRefGoogle Scholar
  12. Klein RR, Rodriguez-Herrera R, Schlueter JA, Klein PE, Yu ZH, Rooney WL (2001) Identification of genomic regions that affect grain-mould incidence and other traits of agronomic importance in sorghum. Theor Appl Genet 102:307–319CrossRefGoogle Scholar
  13. Knoll J, Ejeta G (2008) Marker-assisted selection for early-season cold tolerance in sorghum: QTL validation across populations and environments. Theor Appl Genet 116:541–553PubMedCrossRefGoogle Scholar
  14. Knoll J, Gunaratna N, Ejeta G (2008) QTL analysis of early-season cold tolerance in sorghum. Theor Appl Genet 116:577–587PubMedCrossRefGoogle Scholar
  15. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175CrossRefGoogle Scholar
  16. Lander E, Green P, Abrahamson J, Barlow A, Daly M, Stein L (1987) Mapmaker: an interactive computer package for constructing primary genetic maps of experimental and natural populations. Genomics 1:174–181PubMedCrossRefGoogle Scholar
  17. Lu Q, Dahlberg J (2001) Chinese sorghum genetic resources. Econ Bot 55:401–425CrossRefGoogle Scholar
  18. Lu XL, Niu AL, Cai HY, Zhao Y, Liu JW, Zhu YG, Zhang ZH (2007) Genetic dissection of seedling and early vigor in a recombinant inbred line population of rice. Plant Sci 172:212–220CrossRefGoogle Scholar
  19. Maiti R, Raju P, Bidinger F (1981) Evaluation of visual scoring for seedling vigor in sorghum. Seed Sci Technol 9:613–622Google Scholar
  20. Menz MA, Klein RR, Mullet JE, Obert JA, Unruh NC, Klein PE (2002) A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP, RFLP and SSR markers. Plant Mol Biol 48:483–499PubMedCrossRefGoogle Scholar
  21. Miller FR (1984) Registration of RTx430 sorghum parental line. Crop Sci 24:1224CrossRefGoogle Scholar
  22. Rami J-F, Dufour P, Trouche G, Fliedel G, Mestres C, Davrieux F, Blanchard P, Hamon P (1998) Quantitative trait loci for grain quality, productivity, morphological and agronomical traits in sorghum (Sorghum bicolor L. Moench). Theor Appl Genet 97:605–616CrossRefGoogle Scholar
  23. Salas Fernandez M, Hamblin MT, Li L, Rooney WT, Tuinstra MR, Kresovich S (2008) Quantitative trait loci analysis of endosperm color and carotenoid content in sorghum grain. Crop Sci 48:1732–1743CrossRefGoogle Scholar
  24. Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTL. J Hered 93:77–78PubMedCrossRefGoogle Scholar
  25. Wu YQ, Huang Y (2007) An SSR genetic map of Sorghum bicolor (L.) Moench and its comparison to a published genetic map. Genome 50:84–89PubMedCrossRefGoogle Scholar
  26. Yu J, Tuinstra M (2001) Genetic analysis of seedling growth under cold temperature stress in grain sorghum. Crop Sci 41:1438–1443CrossRefGoogle Scholar

Copyright information

© US Government 2010

Authors and Affiliations

  • Gloria Burow
    • 1
    • 2
  • John J. Burke
    • 1
    • 2
  • Zhanguo Xin
    • 1
  • Cleve D. Franks
    • 1
    • 3
  1. 1.Plant Stress and Germplasm Development UnitCropping Systems Research Laboratory, USDA-ARSLubbockUSA
  2. 2.Department of Plant and Soil ScienceTexas Tech UniversityLubbockUSA
  3. 3.Pioneer Hi-Bred Int’l. Inc.TXUSA

Personalised recommendations