Identification and fine mapping of a resistance gene to Magnaporthe oryzae in a space-induced rice mutant
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Finding novel sources of resistance (R) to rice blast disease should facilitate breeding for improved resistance. The objectives of the present study were to evaluate reactions to blast and identify in a space-induced mutant an R gene to a representative isolate of rice blast pathogen. The mutant H4, its parent and twelve monogenic lines were evaluated for their responses to 35 isolates collected from Guangdong Province, China. H4 was found to be resistant to more isolates than its parent and the twelve monogenic lines, suggesting newly acquired resistance may be a function of one or more R genes. A representative isolate GD0193 was used to identify and map the R gene from H4. Genetic analysis revealed that resistance to the isolate GD0193 was controlled by a single dominant gene, designated Pi46(t). Linkage analysis using susceptible F2 individuals showed that Pi46(t) was mapped between the markers RM224 and RM27360 within 1.04 and 1.2 cM on the long arm of chromosome 11. Subsequently, Pi46(t) was delimited to an interval of approximately 183.7 kb flanked by the markers K67 and T94. These results provide essential information for the cloning of the Pi46(t) gene and will facilitate marker-assisted selection in rice breeding.
KeywordsFine mapping Disease evaluation Rice blast Resistance (R) gene
We thank the Rice Research Institute, Guangdong Academy of Agricultural Sciences, for providing the seeds of Zhonger Ruanzhan. We are grateful to the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, for providing the monogenic lines in this study. This research was supported by the National 863 Program of China (2007AA100101), the National Key Technology Research & Development Program of China (2008BAD97B02), the Natural Science Foundation of Guangdong Province, China (815106400100002), and the earmarked fund for Modern Agro-industry Technology Research System.
- Lee SK, Song MY, Seo YS, Kim HK, Ko S, Cao PJ, Suh JP, Yi G, Roh JH, Lee S, An G, Hahn TR, Wang GL, Ronald P, Jeon JS (2009b) Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes. Genetics 181:1627–1638PubMedCrossRefGoogle Scholar
- Pan QH, Hu ZD, Tanisaka T, Wang L (2003) Fine mapping of the blast resistance gene Pi15, linked to Pii, on rice chromosome 9. Acta Bot Sin 45:871–877Google Scholar
- Shang JJ, Tao Y, Chen XW, Zou Y, Lei CL, Wang J, Li XB, Zhao XF, Zhang MJ, Lu ZK, Xu JC, Cheng ZK, Wan JM, Zhu LM (2009) Identification of a new rice blast resistance gene, Pid3, by genome-wide comparison of paired NBS-LRR genes and their pseudogene alleles between the two sequenced rice genomes. Genetics 182:1303–1311PubMedCrossRefGoogle Scholar
- The Rice Chromosomes 11, 12 Sequencing Consortia (2005) The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications. BMC Biol 3(20):1–18Google Scholar
- Tsunematsu H, Yanoria MJT, Ebron LA, Hayashi N, Ando I, Kato H, Imbe T, Khush GS (2000) Development of monogenic lines of rice for blast resistance. Breed Sci 50:229–234Google Scholar
- Wei LJ, Xu JL, Wang JM, Yang Q, Luo RT, Zhang MX, Bao GL, Sun YQ (2006) A comparative study on mutagenic effects of space flight and irradiation of γ-rays on rice. Agric Sci Chin 5(11):812–819Google Scholar
- Wu JL, Wu CJ, Lei CL, Baraoidan M, Bordeos A, Madamba MRS, Ramous-Pamplona M, Mauleon R, Portugal A, Ulat VJ, Bruskiewich R, Wang GL, Leach J, Khush G, Leung H (2005) Chemical- and irradiation-induced mutants of indica rice IR64 for forward and reverse genetics. Plant Mol Biol 59:85–97PubMedCrossRefGoogle Scholar
- Xiao WM, Yang QY, Chen ZQ, Wang H, Guo T, Liu YZ, Zhu XY (2009) Blast-resistance inheritance of space-induced rice lines and their genomic polymorphism by microsatellite markers. Agric Sci Chin 8(4):101–105Google Scholar
- Zhou B, Qu SH, Liu GF, Dolan M, Sakai H, Lu GD, Bellizzi M, Wang GL (2006) The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea. Mol Plant Microbe Interact 19:1216–1228PubMedCrossRefGoogle Scholar