Molecular markers for tracking variation in lipoxygenase activity in wheat breeding
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Lipoxygenase (LOX) activity is an important factor determining the color of flour and end-use products of wheat. In the present study, quantitative trait loci (QTL) for LOX activity in common wheat were mapped using 71 doubled haploid (DH) lines derived from a Zhongyou 9507 × CA9632 cross, and SSR markers. Two QTL, QLpx.caas.1AL and QLpx.caas-4B, were identified on chromosomes 1AL and 4B, closely associated with LOX activity. The SSR loci Xwmc312 and Xgwm251 proved to be diagnostic and explained 13.4–25.2% of the phenotypic variance for the 1AL locus and 14.3–27.0% for the 4B locus across four environments. The SSR markers Xgwm251 and Xwmc312 were validated across 198 Chinese wheat cultivars and advanced lines and showed highly significant (P < 0.01) association with LOX activity. We further established a multiplexed PCR with SSR marker combination Xwmc312/Xgwm251 to test these wheat cultivars and advanced lines. The results suggested that the marker combination Xwmc312/Xgwm251 is efficient and reliable for evaluating LOX activity and can be used in marker-assisted selection (MAS) for targeting flour color attributes to noodle and other wheat-based products.
KeywordsTriticum aestivum L. Lipoxygenase activity Molecular marker QTL Multiplex PCR
The authors are very grateful to Prof. Robert McIntosh, University of Sydney, for reviewing this manuscript. This study was supported by the National Science Foundation of China (30871516 and 30830072), National Basic Research Program (2009CB118300), National 863 Program (2006AA10Z1A7 and 2006AA100102), international collaboration project from Ministry of Agriculture (2006-G2), and earmarked fund for the Modern Agro-industry Technology Research System.
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