Molecular Breeding

, Volume 28, Issue 1, pp 117–126 | Cite as

Molecular markers for tracking variation in lipoxygenase activity in wheat breeding



Lipoxygenase (LOX) activity is an important factor determining the color of flour and end-use products of wheat. In the present study, quantitative trait loci (QTL) for LOX activity in common wheat were mapped using 71 doubled haploid (DH) lines derived from a Zhongyou 9507 × CA9632 cross, and SSR markers. Two QTL, QLpx.caas.1AL and QLpx.caas-4B, were identified on chromosomes 1AL and 4B, closely associated with LOX activity. The SSR loci Xwmc312 and Xgwm251 proved to be diagnostic and explained 13.4–25.2% of the phenotypic variance for the 1AL locus and 14.3–27.0% for the 4B locus across four environments. The SSR markers Xgwm251 and Xwmc312 were validated across 198 Chinese wheat cultivars and advanced lines and showed highly significant (P < 0.01) association with LOX activity. We further established a multiplexed PCR with SSR marker combination Xwmc312/Xgwm251 to test these wheat cultivars and advanced lines. The results suggested that the marker combination Xwmc312/Xgwm251 is efficient and reliable for evaluating LOX activity and can be used in marker-assisted selection (MAS) for targeting flour color attributes to noodle and other wheat-based products.


Triticum aestivum L. Lipoxygenase activity Molecular marker QTL Multiplex PCR 



The authors are very grateful to Prof. Robert McIntosh, University of Sydney, for reviewing this manuscript. This study was supported by the National Science Foundation of China (30871516 and 30830072), National Basic Research Program (2009CB118300), National 863 Program (2006AA10Z1A7 and 2006AA100102), international collaboration project from Ministry of Agriculture (2006-G2), and earmarked fund for the Modern Agro-industry Technology Research System.

Supplementary material

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(DOC 262 kb)


  1. Allen CL, Lancaster JE, Robinson DS (1999) Lipoxygenase activity in seeds from New Zealand native plants. NZ J Bot 37:737–745CrossRefGoogle Scholar
  2. Bassam BJ, Caetano-Anolles G, Gresshoff PM (1991) Fast, sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83PubMedCrossRefGoogle Scholar
  3. Borrelli GM, Troccoli A, Fonzo ND, Fares C (1999) Durum wheat lipoxygenase activity and other quality parameters that affect pasta color. Cereal Chem 76:335–340CrossRefGoogle Scholar
  4. Carrera A, Echenique V, Zhang W, Helguera M, Manthey F, Schrager A, Picca A, Cervigni G, Dubcovsky J (2007) A deletion at the Lpx-B1 locus is associated with low lipoxygenase activity and improved pasta color in durum wheat. J Cereal Sci 45:67–77CrossRefGoogle Scholar
  5. Fu BX (2008) Asian noodles: history, classification, raw materials, and processing. Food Res Int 41:888–902CrossRefGoogle Scholar
  6. Gokmen V, Serpen A, Atli A, Koksel H (2007) A practical spectrophotometric approach for the determination of lipoxygenase activity of durum wheat. Cereal Chem 84:290–293CrossRefGoogle Scholar
  7. Hart GE, Langston PJ (1977) Chromosomal location and evolution of isozyme structural genes in hexaploid wheat. Heredity 39:263–277CrossRefGoogle Scholar
  8. He ZH, Yang J, Zhang Y, Quail KJ, Peña RJ (2004) Pan bread and dry white Chinese noodle quality in Chinese winter wheats. Euphytica 139:257–267CrossRefGoogle Scholar
  9. Hessler TG, Thomson MJ, Benscher D, Nachit MM, Sorrells ME (2002) Association of a lipoxygenase locus, Lpx-B1, with variation in lipoxygenase activity in durum seeds. Crop Sci 42:1695–1700CrossRefGoogle Scholar
  10. Huang S, Morrison WR (1988) Aspects of protein in Chinese and British common (hexaploid) wheats related to quality of white and yellow Chinese noodles. J Cereal Sci 8:177–187CrossRefGoogle Scholar
  11. Irvine GN, Anderson JA (1953) Variation in principal quality factors of durum wheat with a quality prediction test for wheat or semolina. Cereal Chem 30:334–342Google Scholar
  12. Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175CrossRefGoogle Scholar
  13. Leenhardt F, Lyan B, Rock E, Boussard A, Potus J, Chanliaud E, Remesy C (2006) Genetic variability of carotenoid concentration, and lipoxygenase and peroxidase activities among cultivated wheat species and bread wheat varieties. Eur J Agron 25:170–176CrossRefGoogle Scholar
  14. Li J, Klindworth DL, Shireen F, Cai X, Xu SS (2006) Molecular characterization and chromosome-specific TRAP-marker development for Langdon durum D-genome disomic substitution lines. Genome 49:1545–1554PubMedCrossRefGoogle Scholar
  15. Li HH, Ye GY, Wang JK (2007) A modified algorithm for the improvement of composite interval mapping. Genetics 175:361–374PubMedCrossRefGoogle Scholar
  16. Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with Mapmaker/EXP3.0. Whitehead institute technical reports, 3rd edn. Whitehead Institute, CambridgeGoogle Scholar
  17. Liu JJ, He ZH, Zhao ZD, Peña RJ, Rajaram S (2003) Wheat quality traits and quality parameters of cooked dry white Chinese noodles. Euphytica 131:147–154CrossRefGoogle Scholar
  18. Mackill DJ, Ni JJ (2001) Molecular mapping and marker-assisted selection for major-gene traits in rice. Rice Genet 4:137–151CrossRefGoogle Scholar
  19. McDonald CE (1979) Lipoxygenase and lutenin bleaching activity of durum wheat semolina. Cereal Chem 56:84–89Google Scholar
  20. Mickelson-Young L, Endo TR, Gill BS (1995) A cytogenetic ladder-map of wheat homoeologous group-4 chromosomes. Theor Appl Genet 90:1007–1011CrossRefGoogle Scholar
  21. Miskelly DM (1984) Flour components affecting paste and noodle color. J Sci Food Agric 35:463–471CrossRefGoogle Scholar
  22. Nelson JC, Sorrells ME, Van Deynze AE, Lu Y-H, Atkinson M, Bernard M, Leroy P, Faris JD, Anderson JA (1995) Molecular mapping of wheat: major genes and rearrangements in homoeologous groups 4, 5, and 7. Genetics 141:721–731PubMedGoogle Scholar
  23. Paillard S, Schnurbusch T, Winzeler M, Messmer M, Sourdille P, Abderhalden O, Keller B, Schachermayr G (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.). Theor Appl Genet 107:1235–1242PubMedCrossRefGoogle Scholar
  24. Parker GD, Chalmers KJ, Rathjen AJ, Langridge P (1998) Mapping loci associated with flour color in wheat (Triticum aestivum L.). Theor Appl Genet 97:238–245CrossRefGoogle Scholar
  25. Pastore D, Trono D, Padalino L, Simone S, Valenti D, Fonzo ND, Passarella S (2000) Inhibition by α-tocopherol and L-ascorbate of linoleate hydroperoxidation and β-carotene bleaching activities in durum wheat semolina. J Cereal Sci 31:41–54CrossRefGoogle Scholar
  26. Paterson AH, Damon S, Hewitt JD, Zamir JD, Rabinowitch HD, Lincoln SE, Lander ES, Tanksley SD (1991) Mendelian factors underlying quantitative traits in tomato: comparison across species, generations, and environments. Genetics 127:181–197PubMedGoogle Scholar
  27. Pshenichnikova TA, Osipova SV, Permyakova MD, Mitrofanova TN, Trufanov VA, Lohwasser U, Röder M, Börner A (2008) Mapping of quantitative trait loci (QTL) associated with activity of disulfide reductase and lipoxygenase in grain of bread wheat. Russ J Genet 44:567–574CrossRefGoogle Scholar
  28. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  29. Shiiba K, Nengishi Y, Okada K, Nagao S (1990) Chemical changes during sponge-dough fermentation. Cereal Chem 67:350–355Google Scholar
  30. Shiiba K, Nengishi Y, Okada K, Nagao S (1991) Purification and characterization of lipoxygenase isozymes from wheat germ. Cereal Chem 68:115–122Google Scholar
  31. Siedow JN (1991) Plant lipoxygenase: structure and function. Annu Rev Plant Physiol Plant Mol Biol 42:145–188CrossRefGoogle Scholar
  32. Somers DJ, Fedak G, Savard M (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat. Genome 46:555–564PubMedCrossRefGoogle Scholar
  33. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  34. Trufanov VA, Permyakova MD, Pshenichnikova TA, Ermakova MF, Davydov VA, Prmyakov AV, Berezovskaya EV (2007) The effect of intercultivar substitution of wheat Triticum aestivum L. chromosomes on lipoxygenase activity and its correlation with the technological properties of flour. Appl Biochem Microbiol 43:91–97CrossRefGoogle Scholar
  35. Veldboom LR, Lee M (1996) Genetic mapping of quantitative trait loci in maize in stress and non-stress environments. I. Grain yield and yield components. Crop Sci 36:1310–1319CrossRefGoogle Scholar
  36. Zhang LP, Ge XX, He ZH, Wang DS, Yan J, Xia XC, Sutherland MW (2005) Mapping QTLs for polyphenol oxidase activity in a DH population from common wheat. Acta Agron Sin 31:7–10Google Scholar
  37. Zhao JL, Chen MS, Ma YM, Li RJ, Ren YP, Sun QQ, Li SS (2009) QTL mapping for quality traits of Chinese dry noodle. Agric Sci China 8:394–400CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.College of AgronomyXingjiang Agricultural UniversityUrumqiChina
  2. 2.Institute of Crop Science, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural Sciences (CAAS)BeijingChina
  3. 3.International Maize and Wheat Improvement Center (CIMMYT) China OfficeBeijingChina
  4. 4.Beijing Engineering and Technique Research Center of Hybrid WheatBeijing Academy of Agricultural and Forestry SciencesBeijingChina
  5. 5.Centre for Comparative GenomicsMurdoch UniversityPerthAustralia

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