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Inheritance of growth habit detected by genetic linkage analysis using microsatellites in the common bean (Phaseolus vulgaris L.)

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Abstract

The genetic linkage map for the common bean (Phaseolus vulgaris L.) is a valuable tool for breeding programs. Breeders provide new cultivars that meet the requirements of farmers and consumers, such as seed color, seed size, maturity, and growth habit. A genetic study was conducted to examine the genetics behind certain qualitative traits. Growth habit is usually described as a recessive trait inherited by a single gene, and there is no consensus about the position of the locus. The aim of this study was to develop a new genetic linkage map using genic and genomic microsatellite markers and three morphological traits: growth habit, flower color, and pod tip shape. A mapping population consisting of 380 recombinant F10 lines was generated from IAC-UNA × CAL143. A total of 871 microsatellites were screened for polymorphisms among the parents, and a linkage map was obtained with 198 mapped microsatellites. The total map length was 1865.9 cM, and the average distance between markers was 9.4 cM. Flower color and pod tip shape were mapped and segregated at Mendelian ratios, as expected. The segregation ratio and linkage data analyses indicated that the determinacy growth habit was inherited as two independent and dominant genes, and a genetic model is proposed for this trait.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

CTAB:

Cetyltrimethylammonium bromide

CNL:

Coiled-coil nucleotide-binding site leucine-rich repeat

EST:

Expressed sequence tag

IAC:

Agronomic Institute of Campinas

PCR:

Polymerase chain reaction

RAPD:

Random amplified polymorphic DNA

RIL:

Recombinant inbred line

RFLP:

Restriction fragment length polymorphism

SSR:

Single sequence repeat

UC:

IAC-UNA × CAL143

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Acknowledgments

This work was supported by grants from State of São Paulo Research Foundation (FAPESP). The authors are also grateful Brazilian National Council for Scientific and Technological Development (CNPq) for a graduate fellowship to C.T. and grants to A.P.S.; FAPESP for graduate fellowship to P.R.O., J.M.K.C., R.M.B., A.C.B.S. and an undergraduate fellowship to D.A.S.

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Authors and Affiliations

  1. Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, CP 6010, Campinas, SP, CEP 13083-970, Brazil

    Tatiana de Campos, Danilo Augusto Sforça, Adna Cristina Barbosa de Sousa & Anete Pereira de Souza

  2. Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais, Fazenda Santa Elisa, IAC, Campinas, SP, Brazil

    Paula Rodrigues Oblessuc, Juliana Morini Kupper Cardoso, Renata Moro Baroni, Sérgio Augusto Morais Carbonell, Alisson Fernando Chioratto & Luciana Benchimol Rubiano

  3. Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz (ESALQ), Universidade de São Paulo (USP), CP 83, Piracicaba, SP, CEP 13400-970, Brazil

    Antonio Augusto Franco Garcia

  4. Departamento de Biologia Vegetal—Instituto de Biologia (IB), UNICAMP, Cidade Universitária Zeferino Vaz, CP 6010, Campinas, SP, CEP 13083-970, Brazil

    Anete Pereira de Souza

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  1. Tatiana de Campos
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  2. Paula Rodrigues Oblessuc
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  3. Danilo Augusto Sforça
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  4. Juliana Morini Kupper Cardoso
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  5. Renata Moro Baroni
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  6. Adna Cristina Barbosa de Sousa
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  7. Sérgio Augusto Morais Carbonell
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  8. Alisson Fernando Chioratto
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  9. Antonio Augusto Franco Garcia
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  10. Luciana Benchimol Rubiano
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  11. Anete Pereira de Souza
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Correspondence to Anete Pereira de Souza.

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de Campos, T., Oblessuc, P.R., Sforça, D.A. et al. Inheritance of growth habit detected by genetic linkage analysis using microsatellites in the common bean (Phaseolus vulgaris L.). Mol Breeding 27, 549–560 (2011). https://doi.org/10.1007/s11032-010-9453-x

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