Molecular Breeding

, Volume 27, Issue 3, pp 301–314 | Cite as

Mapping of quantitative trait loci associated with protein expression variation in barley grains

  • Katja Witzel
  • Christof Pietsch
  • Marc Strickert
  • Andrea Matros
  • Marion S. Röder
  • Winfriede Weschke
  • Ulrich Wobus
  • Hans-Peter Mock
Article

Abstract

Barley (Hordeum vulgare) is an important cereal crop grown for both the feed and malting industries. Hence, there is great interest to gain deeper insight into the determinants of grain nutritional quality in order to improve the assessment of new traits. Two-dimensional gel electrophoresis was employed for the characterization of the grain proteome of doubled-haploid introgression lines (IL) representing a wild barley genome (Hordeum spontaneum Hs213) within a modern cultivar background (H. vulgare cv. Brenda). Proteome maps were subjected to differential cluster analysis and revealed ILs with similar or different protein expression patterns compared to the Brenda parent. A total of 51 quantitative trait loci for protein expression (pQTL) were detected, and proteins underlying these pQTL were further examined by mass spectrometry. Identification was successful for 49 of the segregating spots and functional annotation of proteins revealed that most proteins are involved in metabolism and disease/defence-related processes. Among those, multigene families of glyceraldehyde-3-phosphate dehydrogenases, heat shock proteins, peroxidases, and serpins were identified. Overall, eight pQTL signals were discovered in two independently grown sets of plants. The mapped spots included protein disulfide isomerase, α-amylase inhibitor BDAI, NADP malic enzyme, adenosine kinase and peroxidase BP1. Specific marker information of proteins involved in developmental events and protein storage as well as in disease- and defence-related processes now allows for targeted breeding approaches to improve the grain quality in barley.

Keywords

2-D gel electrophoresis QTL analysis Proteome analysis Mass spectrometry Barley grain 

Abbreviations

2D GE

2-Dimensional gel electrophoresis

AB

Advanced backcross

BDAI

α-Amylase inhibitor

BP1

Barley peroxidase 1

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

GlcRibDH

Glucose and ribitol dehydrogenase

IL

Introgression line

IPG

Immobilized pH gradient

MS

Mass spectrometry

QTL

Quantitative trait loci

Notes

Acknowledgments

We thank Annegret Wolf for excellent technical assistance. This work was supported by the German Federal Ministry of Education and Research (GABISEED II; FKZ 0313115). We greatly appreciate the valuable comments of two anonymous reviewers.

Supplementary material

11032_2010_9432_MOESM1_ESM.pdf (530 kb)
PDF 531 kb

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Katja Witzel
    • 1
    • 2
  • Christof Pietsch
    • 1
    • 3
  • Marc Strickert
    • 1
    • 4
  • Andrea Matros
    • 1
  • Marion S. Röder
    • 1
  • Winfriede Weschke
    • 1
  • Ulrich Wobus
    • 1
  • Hans-Peter Mock
    • 1
  1. 1.Leibniz Institute of Plant Genetics and Crop Plant ResearchGaterslebenGermany
  2. 2.Institute of Plant Nutrition and Soil ScienceChristian-Albrechts-UniversityKielGermany
  3. 3.KWS LOCHOW GMBHEinbeckGermany
  4. 4.University of SiegenSiegenGermany

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