Molecular Breeding

, Volume 26, Issue 4, pp 667–680 | Cite as

Haplotype diversity of stem rust resistance loci in uncharacterized wheat lines

  • Long-Xi Yu
  • Sixin Liu
  • James A. Anderson
  • Ravi P. Singh
  • Yue Jin
  • Jorge Dubcovsky
  • Gina Brown-Guidera
  • Sridhar Bhavani
  • Alexey Morgounov
  • Zhonghu He
  • Julio Huerta-Espino
  • Mark E. Sorrells
Article

Abstract

Stem rust is one of the most destructive diseases of wheat worldwide. The recent emergence of wheat stem rust race Ug99 (TTKS based on the North American stem rust race nomenclature system) and related strains threaten global wheat production because they overcome widely used genes that had been effective for many years. Host resistance is likely to be more durable when several stem rust resistance genes are pyramided in a single wheat variety; however, little is known about the resistance genotypes of widely used wheat germplasm. In this study, a diverse collection of wheat germplasm was haplotyped for stem rust resistance genes Sr2, Sr22, Sr24, Sr25, Sr26, Sr36, Sr40, and 1A.1R using linked microsatellite or simple sequence repeat (SSR) and sequence tagged site (STS) markers. Haplotype analysis indicated that 83 out of 115 current wheat breeding lines from the International Maize and Wheat Improvement Center (CIMMYT) likely carry Sr2. Among those, five out of 94 CIMMYT spring lines tested had both Sr2 and Sr25 haplotypes. Five out of 22 Agriculture Research Service (ARS) lines likely have Sr2 and a few have Sr24, Sr36, and 1A.1R. Two out of 43 Chinese accessions have Sr2. No line was found to have the Sr26 and Sr40 haplotypes in this panel of accessions. DArT genotyping was used to identify new markers associated with the major stem resistance genes. Four DArT markers were significantly associated with Sr2 and one with Sr25. Principal component analysis grouped wheat lines from similar origins. Almost all CIMMYT spring wheats were clustered together as a large group and separated from the winter wheats. The results provide useful information for stem rust resistance breeding and pyramiding.

Keywords

Stem rust Sr gene Haplotype Pyramiding Genetic relationship Marker-assisted selection 

Supplementary material

11032_2010_9403_MOESM1_ESM.doc (232 kb)
Supplementary material 1 (DOC 232 kb)

References

  1. Akbari M, Wenzl P, Caig V et al (2006) Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theor Appl Genet 113:1409–1420PubMedCrossRefGoogle Scholar
  2. Ayala-Navarrete L, Bariana HS, Singh RP, Gibson JM, Mechanicos AA, Larkin PJ (2007) Trigenomic chromosomes by recombination of Thinopyrum intermedium and Th. ponticum translocations in wheat. Theor Appl Genet 116:63–75PubMedCrossRefGoogle Scholar
  3. Bariana HS, Brown GN, Bansal UK, Miah H, Standen GE, Lu M (2007) Breeding triple rust resistant wheat cultivars for Australia using conventional and marker-assisted selection technologies. Aust J Agric Res 58:576–587CrossRefGoogle Scholar
  4. Dundas IS, Anugrahwati DR, Verlin DC, Park RF, Bariana HS, Mago R, Islam AKMR (2007) New sources of rust resistance from alien species: meliorating linked defects and discovery. Aust J Agric Res 58:545–549CrossRefGoogle Scholar
  5. Dyck PL (1992) Transfer of a gene for stem rust resistance from Triticum araraticum to hexaploid wheat. Genome 35:788–792CrossRefGoogle Scholar
  6. Friebe B, Jiang J, Knott DR, Gill BS (1994) Compensation indexes of radiation-induced wheat Agropyron-elongatum translocations conferring resistance to leaf rust and stem rust. Crop Sci 34:400–404CrossRefGoogle Scholar
  7. Gerechter-Amitai ZK, Wahl I, Vardi A, Zohary D (1971) Transfer of stem rust seedling resistance from wild diploid einkorn to tetraploid durum wheat by means of a triploid hybrid bridge. Euphytica 2:281–285CrossRefGoogle Scholar
  8. Gyarfas J (1978) Transference of disease resistance from Triticum timopheevii to Triticum aestivum. Master’s thesis. University of Sydney, AustraliaGoogle Scholar
  9. Hare RA, McIntosh RA (1979) Genetic and cytogenetic studies of durable, adult-plant resistances in Hope and related cultivars to rusts. Z Planzen 83:350–367Google Scholar
  10. Heffner EL, Sorrells ME, Jannink J-L (2009) Genomic selection for crop improvement. Crop Sci 49:1–12CrossRefGoogle Scholar
  11. Heun M, Kennedy AE, Anderson JA, Lapitan NLV, Sorrells ME, Tanksley SD (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 34:437–447CrossRefGoogle Scholar
  12. Jiang J, Friebe B, Gill BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73:199–212CrossRefGoogle Scholar
  13. Jin Y, Singh RP, Ward RW, Wangyera R, Kinyua M, Njau P, Fetch T, Pretorius ZA, Yahyaoui A (2007) Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 91:1096–1099CrossRefGoogle Scholar
  14. Jin Y, Szabo LJ, Pretorius ZA, Singh RP, Ward RW, Fetch TJ (2008) Detection of virulence to resistance gene Sr24 within race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 92:923–926CrossRefGoogle Scholar
  15. Jin Y, Szabo LJ, Rouse MN, Fetch T Jr, Pretorius ZA, Wanyera R, Njau P (2009) Detection of virulence to resistance gene Sr36 within the TTKS race lineage of Puccinia graminis f. sp tritici. Plant Dis 93:367–370CrossRefGoogle Scholar
  16. Joshi LM, Palmer LT (1973) Epidemiology of stem, leaf and stripe rusts of wheat in Northern India. Plant Dis Rep 57:8–12Google Scholar
  17. Kerber ER, Dyck PL (1973) Inheritance of stem rust resistance transferred from diploid wheat (Triticum monococcum) to tetraploid and hexaploid wheat and chromosome location of the gene involved. Can J Genet Cytol 15:397–409Google Scholar
  18. Khan R, Bariana H, Dholakia B, Naik S, Lagu M, Rathjen A, Bhavani S, Gupta V (2005) Molecular mapping of stem and leaf rust resistance in wheat. Theor Appl Genet 111:846–850PubMedCrossRefGoogle Scholar
  19. Knott DR (1980) Mutation of a gene for yellow pigment linked to Lr19 in wheat. Can J Genet Cytol 22:651–654Google Scholar
  20. Leonard KJ (2001a) Black stem rust biology and threat to wheat growers. The Central Plant Board Meeting, LexingtonGoogle Scholar
  21. Leonard KJ (2001b) Stem rust-future enemy? In: Peterson PD (ed) Stem rust of wheat: from ancient enemy to modern Foe. pp 119–146Google Scholar
  22. Liu S, Yu L-X, Singh RP, Jin Y, Sorrells ME, Anderson JA (2010) Diagnostic and co-dominant PCR markers for wheat stem rust resistance genes Sr25 and Sr26. Theor Appl Genet 120:691–697Google Scholar
  23. Mago R, Bariana HS, Dundas IS, Spielmeyer W, Lawrence GJ, Pryor AJ, Ellis JG (2005) Development of PCR markers for the selection of wheat stem rust resistance genes Sr24 and Sr26 in diverse wheat germplasm. Theor Appl Genet 111:496–504PubMedCrossRefGoogle Scholar
  24. Martin RH (1971) Eagle-a new wheat variety. Agric Gaz NSW 82:206–207Google Scholar
  25. McFadden ES (1930) A successful transfer of emmer characters to vulgare wheat. Agron J 22:1020–1034CrossRefGoogle Scholar
  26. McIntosh RA, Gyarfas J (1971) Triticum timopheevii as a source of resistance to wheat stem rust. Z Pflanzenzüchtg 66:240–248Google Scholar
  27. McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts, an atlas of resistance genes. CSIRO, MelbourneGoogle Scholar
  28. McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers WJ, Morris CF, Somers D, Appels R, Devos KM (2008) Catalogue of gene symbols for wheat. In: McIntosh RA (ed), Gene symbols. http://wheat.pw.usda.gov/GG2/Triticum/wgc/2008/GeneSymbol.pdf
  29. Miranda LM, Perugini L, Srnić G, Brown-Guedira G, Marshall D, Leath S, Murphy JP (2007) Genetic mapping of a Triticum monococcum derived powdery mildew resistance gene in common wheat. Crop Sci 47:2323–2329CrossRefGoogle Scholar
  30. Olson EL, Brown-Guedira G, Marshall DS, Jin Y, Mergoum M, Lowe I, Dubcovsky J (2010) Genotyping of U.S. wheat germplasm for presence of stem rust resistance genes Sr24, Sr36 and Sr1RSAmigo. Crop Sci 50:1–8CrossRefGoogle Scholar
  31. Pretorius ZA, Singh RP, Wagoire WW, Payne TS (2000) Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f. sp. Plant Dis 84:203CrossRefGoogle Scholar
  32. Prins R, Groenewald JZ, Marais GF, Snape JW, Koebner RMD (2001) AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor App Genet 103:618–624CrossRefGoogle Scholar
  33. Rees RG (1972) Uredospore movement and observations on the epidemiology of wheat rusts in north-eastern Australia. Agric Res 23:215–223CrossRefGoogle Scholar
  34. Roelfs AP, McVey DV (1979) Low infection types produced by Puccinia graminis f. sp. tritici and wheat lines with designated genes for resistance. Phytopathology 69:722–730CrossRefGoogle Scholar
  35. Saal B, Wricke G (1999) Development of simple sequence repeat markers in rye (Seale cereale L.). Genome 42:964–972PubMedGoogle Scholar
  36. Singh RP, Hodson DP, Jin Y, Huerta-Espino J et al (2006) Current status, likely migration and strategies to mitigate the threat to wheat production from race Ug99 (TTKS) of stem rust pathogen. CAP Rev: Perspect Agri, Vet Sci, Nutr Nat Resour 1:1–13Google Scholar
  37. Singh RP, Hodson DP, Huerta-Espino J, Jin Y et al (2008) Will stem rust destroy the world’s wheat crop? Adv Agron 98:271–309CrossRefGoogle Scholar
  38. Singh RP, Huerta-Espino J, Bhavani S, Singh D, Singh PK, Herrera-Foessel SA, Njau P, Wanyera R, Jin Y (2009) Breeding for minor gene-based resistance to stem rust of wheat. Proceedings of Borlaug Global Rust Initiative, C.D. ObregonGoogle Scholar
  39. Smith EL, Schlehubber AM, Young HCJ, Edwards LH (1968) Registration of ‘Agent’ wheat. Crop Sci 8:511–512CrossRefGoogle Scholar
  40. Sneath PHA, Sokal RR (1973) Numerical taxonomy—the principles and practice of numerical classification. W. H. Freeman, San FranciscoGoogle Scholar
  41. Spielmeyer W, Sharp PJ, Lagudah ES (2003) Identification and validation of markers linked to broad-spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.). Crop Sci 43:333–336CrossRefGoogle Scholar
  42. The TT, Latter BDH, McIntosh RA, Ellison FW, Brennan PS, Fisher J, Hollamby GJ, Rathjen AJ, Wilson RE (1988) Grain yields of near isogenic lines with added genes for stem rust resistance. In: Miller TE, Koebner RMD (eds) Proceedings of 7th international wheat genetics symposium. Bath Pres, Bath, pp. 901–909Google Scholar
  43. The TT, Gupta RB, Dyck PL, Applels R, Hohmann U, McIntosh RA (1992) Characterization of stem rust resistance derivatives of wheat variety Amigo. Euphytica 58:245–252CrossRefGoogle Scholar
  44. Tsilo TJ, Jin Y, Anderson JA (2008) Diagnostic microsatellite markers for the detection of stem rust resistance gene Sr36 in diverse genetic backgrounds of wheat. Crop Sci 48:253–261CrossRefGoogle Scholar
  45. Wu S, Pumphrey M, Bai G (2009) Molecular mapping of stem-rust-resistance gene Sr40 in wheat. Crop Sci 49:1682–1686CrossRefGoogle Scholar
  46. Yu L-X, Z. Abate Z, Anderson JA, Bansal UK, Bariana HS, Bhavani S, Dubcovsky J, Lagudah ES, Liu S, Sambasivam PK, Singh RP, Sorrells ME (2009) Developing and optimizing markers for stem rust resistance in wheat. Proceedings of Borlaug Global Rust Initiative. CD. Obregon, P39–P56Google Scholar
  47. Zadoks JC (1963) Epidemiology of wheat rust in Europe. FAO Plant Prot Bull 13:97–108Google Scholar
  48. Zhang W, Dubcovsky J (2008) Association between allelic variation at the Phytoene synthase 1 gene and yellow pigment content in the wheat grain. Theor Appl Genet 116:635–645PubMedCrossRefGoogle Scholar
  49. Zhang W, Lukaszewski AJ, Kolmer J, Soria MA, Goyal S, Dubcovsky J (2005) Molecular characterization of durum and common wheat recombinant lines carrying leaf rust resistance (Lr19) and yellow pigment (Y) genes from Lophopyrumponticum. Theor Appl Genet 111:573–582PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Long-Xi Yu
    • 1
  • Sixin Liu
    • 2
  • James A. Anderson
    • 2
  • Ravi P. Singh
    • 3
  • Yue Jin
    • 4
  • Jorge Dubcovsky
    • 5
  • Gina Brown-Guidera
    • 6
  • Sridhar Bhavani
    • 3
  • Alexey Morgounov
    • 3
  • Zhonghu He
    • 3
    • 7
  • Julio Huerta-Espino
    • 8
  • Mark E. Sorrells
    • 1
  1. 1.Department of Plant Breeding and GeneticsCornell UniversityIthacaUSA
  2. 2.Department of Agronomy and Plant GeneticsUniversity of MinnesotaSt. PaulUSA
  3. 3.International Maize and Wheat Improvement Center (CIMMYT)Edo MexMexico
  4. 4.USDA-ARSCereal Disease LaboratorySt. PaulUSA
  5. 5.Department of Plant SciencesUniversity of CaliforniaDavisUSA
  6. 6.USDA-ARS Plant Science ResearchRaleighUSA
  7. 7.Chinese Academy of Agriculture ScienceBeijingChina
  8. 8.Campo Experimental Valle de México INIFAPChapingoMexico

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