Association mapping of late maturity α-amylase (LMA) activity in a collection of synthetic hexaploid wheat
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Late maturity α-amylase (LMA) is a genetic defect of wheat which results in the production of α-amylase, shown as substandard falling numbers, in the absence of preharvest rain and under cool temperatures during ripening. The present study is an attempt to use a whole-genome scan with DArT markers to identify chromosomal regions influencing LMA in synthetic hexaploid wheat (SHW). A high heritability estimate of 86.6% was calculated for LMA phenotype measured as optical density in a collection of 91 SHWs. Linkage disequilibrium extended up to 10 cM, and with controls for false positives, significant markers were detected at the chromosome 7B region previously linked to LMA in bread wheat, but not at the chromosome 3B region. Of potentially great interest is a region on chromosome 6B, which was identified as having a significant association with LMA phenotypes in the SHW accessions. Previous investigations suggested existence of an LMA gene on the long arm of 6B, but this is the first time it has been mapped to lie within the centromeric region of chromosome 6B, a region that harbours the Amy-1 genes and whose expression governs activity of the high pI α-amylase isoenzymes.
KeywordsSynthetic hexaploid wheat Late maturity α-amylase DArT markers Association mapping
This work was supported by grants from the Grains Research and Development Corporation (GRDC) and NSW Department of Primary Industries. The authors acknowledge contributions made by Francis Ogbonnaya to the CIMMYT-Australian Germplasm Evaluation (CAGE) suite of projects.
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