Molecular Breeding

, Volume 25, Issue 4, pp 675–684

Development and polymorphism of Vigna unguiculata ssp. unguiculata microsatellite markers used for phylogenetic analysis in asparagus bean (Vigna unguiculata ssp. sesquipedialis (L.) Verdc.)

  • Pei Xu
  • Xiaohua Wu
  • Baogen Wang
  • Yonghua Liu
  • Dehui Qin
  • Jeffery D. Ehlers
  • Timothy J. Close
  • Tingting Hu
  • Zhongfu Lu
  • Guojing Li
Article

DOI: 10.1007/s11032-009-9364-x

Cite this article as:
Xu, P., Wu, X., Wang, B. et al. Mol Breeding (2010) 25: 675. doi:10.1007/s11032-009-9364-x

Abstract

Asparagus bean (V. unguiculata ssp. sesquipedialis), a specific form of cowpea (V. unguiculata L. Walp.), is cultivated as a vegetable crop throughout eastern and southern Asia for its tender long pods. Little is known about the genetic relationship between asparagus bean and the broader species, particularly the dominant ssp. unguiculata. We report here the development and transferability of simple sequence repeat (SSR) markers, over 40% of which are EST-derived, from ssp. unguiculata to asparagus bean and the use of a subset of the polymorphic markers to assess the genetic diversity of asparagus bean cultivars from diverse geographic origins across China. A total of 410 EST derived SSR (eSSR) markers and 600 SSR markers derived from cowpea genespace sequences (GSS) were developed, with a cross-subspecies transferability of 100% and 98.5%, respectively. In a recombinant inbred line population of asparagus bean, a 1:1 segregation was observed for most loci. Principal coordinate analysis (PCA) and phylogenetic clustering based on 62 alleles detected by 14 polymorphic SSR markers distinguished ssp. unguiculata and sesquipedialis into separate groups. Improved asparagus bean cultivars in China generally have a narrow genetic basis compared with landraces varieties. This suggests that asparagus bean breeding programs need to consider utilizing landrace germplasm to enhance genetic variability and ensure long-term gains from selection and reduce genetic vulnerability to pathogen/pest epidemics. Because of their transferability across subspecies, the SSR markers described in this study could be effectively employed in cross-subspecies trait introgression breeding from ssp. unguiculata to sesquipedialis.

Keywords

Asparagus bean Cowpea Genetic diversity RIL SSR Vigna 

Abbreviations

AFLP

Amplified fragment length polymorphism

EST

Expressed sequence tag

GSS

Gene space sequence

PCA

Principal coordinate analysis

PIC

Polymorphism information content

RAPD

Rapid amplification polymorphism of DNA

RFLP

Restriction fragment length polymorphism

RIL

Recombinant inbred line

SFP

Single feature polymorphisms

SSR

Simple sequence repeat

Supplementary material

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Pei Xu
    • 1
  • Xiaohua Wu
    • 1
  • Baogen Wang
    • 1
  • Yonghua Liu
    • 1
  • Dehui Qin
    • 2
  • Jeffery D. Ehlers
    • 3
  • Timothy J. Close
    • 3
  • Tingting Hu
    • 1
  • Zhongfu Lu
    • 1
  • Guojing Li
    • 1
  1. 1.Institute of VegetablesZhejiang Academy of Agricultural SciencesHangzhouPeople’s Republic of China
  2. 2.Institute of HorticultureZhejiang Academy of Agricultural SciencesHangzhouPeople’s Republic of China
  3. 3.Department of Botany and Plant SciencesUniversity of CaliforniaRiversideUSA

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