Application of high-resolution DNA melting for genotyping and variant scanning of diploid and autotetraploid potato
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The ideal marker system for tetraploid potato would be dosage-sensitive and have the ability to distinguish heterozygous genotypes with multiple haplotypes within the genomic region targeted by the marker. The objective of this study was to evaluate the utility of high-resolution DNA melting (HRM) for genotyping and polymorphism detection in diploid and tetraploid potato. Amplicon scanning, unlabelled probe, and short amplicon assays were developed for four candidate genes affecting tuber skin and flesh colour, and starch, and a marker linked to nematode resistance. Genotyping a set of 95 potato clones revealed several examples of clones with three distinct haplotypes. Combined probe and amplicon analysis identified between 29 and 44 unique genotypes for the same assays. Assays developed for four of the five target genes are suitable for marker-assisted selection in potato breeding programs. This study illustrates the use of HRM in potato genetics. Further advances in the technology and associated data analysis should make HRM a useful tool for basic and applied studies of potato.
KeywordsPotato High-resolution DNA melting Haplotype dosage Autotetraploid genetics Marker-assisted selection Variant scanning
We thank Valerie Burns and Stephen Allaby for maintaining the plants and for providing trait descriptions. This work was supported, in part, with funds from the New Brunswick Department of Agriculture and Aquaculture Embracing Innovation in Agriculture program.
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