Molecular Breeding

, Volume 23, Issue 2, pp 209–217 | Cite as

A novel codominant marker for selection of the null Wx-B1 allele in wheat breeding programs

  • Mika Saito
  • Patricia Vrinten
  • Goro Ishikawa
  • Robert Graybosch
  • Toshiki Nakamura
Article
  • 291 Downloads

Abstract

Waxy protein (granule-bound starch synthase I) is a key enzyme in the synthesis of amylose in endosperm tissue. The amylose content of wheat flour plays a significant role in determining Japanese udon noodle quality. Most wheat cultivars suitable for producing udon noodles have a low amylose level due to a lack of Wx-B1 protein conditioned by null Wx-B1 alleles. It was previously determined that the entire coding region of the wheat Wx-B1 gene is deleted in the most common null allele. However, the extent and breakpoints of the deletion have not been established. In this study, the position of the 3′ deletion breakpoint was refined by mapping with PCR-based markers. Using information from this analysis, a chromosome walk was initiated and the DNA sequence flanking the deletion breakpoints was obtained. The deletion included a 3,872 bp region downstream from the termination codon of Wx-B1 gene. Based on similarity with T. monococcum sequences, it was estimated that approximately 60 kb upstream of the Wx-B1 gene was also deleted. Using this sequence information, a codominant marker for the identification of the Wx-B1 null allele was developed. This marker can unambiguously identify heterozygous plants, which will accelerate the selection of partial waxy mutants carrying the Wx-B1 null allele.

Keywords

Wx-B1 Wheat Codominant marker Breeding 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Mika Saito
    • 1
    • 2
  • Patricia Vrinten
    • 3
  • Goro Ishikawa
    • 2
  • Robert Graybosch
    • 4
  • Toshiki Nakamura
    • 2
  1. 1.Central LaboratoryNippon Flour Mills Co., Ltd.AtsugiJapan
  2. 2.Tohoku National Agriculture Research CenterMoriokaJapan
  3. 3.Bioriginal Food & Science Corp.SaskatoonCanada
  4. 4.USDA-ARS, 362C Plant Science HallUniversity of NebraskaLincolnUSA

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