A new PCR-based marker on chromosome 4AL for resistance to pre-harvest sprouting in wheat (Triticum aestivum L.)
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Pre-harvest sprouting (PHS) is a complex trait controlled by multiple genes with strong interaction between environment and genotype that makes it difficult to select breeding materials by phenotypic assessment. One of the most important genes for pre-harvest sprouting resistance is consistently identified on the long arm of chromosome 4A. The 4AL PHS tolerance gene has therefore been targeted by Australian white-grained wheat breeders. A new robust PCR marker for the PHS QTL on wheat chromosome 4AL based on candidate genes search was developed in this study. The new marker was mapped on 4AL deletion bin 13-0.59-0.66 using 4AL deletion lines derived from Chinese Spring. This marker is located on 4AL between molecular markers Xbarc170 and Xwg622 in the doubled-haploid wheat population Cranbrook × Halberd. It was mapped between molecular markers Xbarc170 and Xgwm269 that have been previously shown to be closely linked to grain dormancy in the doubled haploid wheat population SW95-50213 × Cunningham and was co-located with Xgwm269 in population Janz × AUS1408. This marker offers an additional efficient tool for marker-assisted selection of dormancy for white-grained wheat breeding. Comparative analysis indicated that the wheat chromosome 4AL QTL for seed dormancy and PHS resistance is homologous with the barley QTL on chromosome 5HL controlling seed dormancy and PHS resistance. This marker will facilitate identification of the gene associated with the 4A QTL that controls a major component of grain dormancy and PHS resistance.
KeywordsMapping Marker-assisted selection Pre-harvest sprouting Quantitative trait loci (QTL) Seed dormancy White-grained wheat (Triticum aestivum L. em Thell.)
We thank Ms Fiona Drake-Brockman and Ms. Esther Walker of Department of Agriculture & Food Western Australia for providing us with the DNA samples of DH population Cranbrook × Halberd, and DNA samples of Chinese Spring derived wheat nullisomic-tetrasomic (NT) lines and deletion bin lines. We thank Dr. Biddulph of Department of Agriculture & Food Western Australia and Dr. Lu of Sydney University for providing us phenotype information for some wheat breeding lines. This work is within the project “Improvement of malting barley pre-harvest sprouting tolerance through molecular techniques” supported by Molecular Plant Breeding Cooperative Research Centre, Australia.
- Chalmers KJ, Campbell AW, Kretschmer J, Karakousis A, Henschke PH, Pierens S, Harker N, Pallotta M, Cornish GB, Shariflou MR, Rampling LR, McLauchlan A, Daggard G, Sharp PJ, Holton TA, Sutherland MW, Appels R, Langridge P (2001) Construction of three linkage maps in bread wheat, Triticum aestivum. Aust J Agri Res 52:1089–1119CrossRefGoogle Scholar
- Flintham J, Adlam RE, Gale MD (1999) Seedcoat and embryo dormancy in wheat. In: Weipert D (ed) Eighth international symposium on pre-harvest sprouting in cereals 1998’. Association of Cereal Research Federal Centre for Cereal Potato and Lipid Research. Detmold, Germany, pp 67–76Google Scholar
- Gupta PK, Balyan HS, Edwards KJ, Isaac P, Korzun V, Röder M, Gautier MF, Joudrier P, Schlatter AR, Dubcovsky J, DelaPena RC, Khairallah M, Penner G, Hayden MJ, Sharp P, Keller B, Wang RCC, Hardouin JP, Jack P, Leroy P (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422PubMedCrossRefGoogle Scholar
- Mares DJ (1987) Pre-harvest sprouting tolerance in white-grained wheat. In: Mares SJ (ed) Fourth international symposium on preharvest sprouting in cereals, Westview Press, Boulder pp 64–74Google Scholar
- Mares DJ (1996) Dormancy in white wheat: mechanism and location of genes. In: Noda K, Mares DJ (eds) Preharvest sprouting in cereals 1995’. Centre for Academic Societies, Osaka, Japan, pp 179–184Google Scholar
- Mares DJ (1999) The seed coat and dormancy in wheat grains. In: Weipert D (ed) Eighth international symposium on pre-harvest sprouting in cereals 1998’. Association of Cereal Research Federal Centre for Cereal Potato and Lipid Research. Detmold, Germany, pp 77–81Google Scholar
- Mares DJ, Ellison FW (1990) Dormancy and preharvest sprouting tolerance in white-grained and red-grained wheats. In: Ringlund K, Mosleth E, Mares DJ (eds) Fifth international symposium on preharvest sprouting in cereals. Westview Press Inc., Boulder, Co., USA, pp 75-84Google Scholar
- Yang J, Hu CC, Ye XZ, Zhu J (2005) QTL Network 2.0. Institute of Bioinformatics, Zhejiang University, Hangzhou, China. (http://ibi.zju.edu.en/software/qtlnetwork)Google Scholar
- Zhang XQ, Li C, Tay A, Westcott S, Lance R, Appels R (2007) Dissecting a region of barley chromosome 5HL using rice genomic sequences as references. The 13th Australian Barley Technical Symposium, Fremantle, Western Australia, Aug 26–29, 2007Google Scholar